关键词: 野生大豆, NaCl胁迫, 生理生化性状, Na+ / K+

Abstract: High salinity stress adversely affects plant growth and development. Compared with cultivated soybean, the wild soybean has more salt-tolerant genes in genome because of its broad distribution, the large genetic diversity and the wide adaptation to different environments. The comparative analysis of physiological and biochemical characters of the salt resistant wild soybean ST-335 and salt sensitive one SS-736 were carried out in this study. The effects of some genes mediating Na+ and K+ homeostasis on soybean salt tolerance were analyzed by overexpressing genes in soybean hair roots. The results showed that the content of soluble sugar and the activities of SOD, POD,CAT were higher in ST-335, Na+/K+ ratio was lower in ST-335. The relative expressions of KOR，NHX and NSCC were lower and the relative expressions of SKOR and SOS1 were higher in ST-335 than that in SS-736. The overexpression of SOS1 and SKOR in soybean hair root enhanced the salt tolerance in soybean composite plants, while overexpression of NSCC decreased the salt tolerance in soybean. These results suggested that the oxidative stress tolerance and ion balance in ST-335 were better than that in SS-735. The results of this study provided theoretical basis for deciphering the salt tolerance mechanism and selecting salt tolerant germplasm of wild soybean.

Key words: wild soybean, NaCl stress, physi-biochemical traits, Na+/ K+