关键词: VIGS, 广藿香, 广藿香醇合酶, 倍半萜类化合物

Abstract: The patchouli oil is mainly stored in the peltate trichome on the aerial part of patchouli. The total RNA was extracted from the leaf and the complete cDNA sequence of patchoulol synthase gene (PatPTS) was cloned via PCR. The virus induced gene silencing(VIGS) system was constructed in patchouli to characterize the function of PatPTS, and the tobacco rattle virus (TRV) was used to silence PatPTS expression. GC-MS and qRT-PCR were used to detect the variations of these sesquiterpenes and PatPTS transcription in leaf after VIGS infection. The contents of β-patchoulene, β-elemene, trans-β-caryophyllene and (-)-patchoulol were decreased significantly after infection for 2 weeks, and the expression level of PatPTS showed similar pattern; which meaned PatPTS was in charge of the biosynthesis of the major components of patchouli oil. The results indicated that the VIGS system in patchouli was successfully established, and silence effects on secondary metabolites production and gene expression were the most effective after infection for 2 weeks, which provided an effective method for the fast screen of gene functions.

Key words: VIGS, Pogostemon cablin, PatPTS, sesquiterpenes