小麦氮高效基因型的蛭石盒筛选

doi:10.13304/j.nykjdb.2015.166

›› 2015, Vol. 17 ›› Issue (4): 129-134.DOI: 10.13304/j.nykjdb.2015.166

小麦氮高效基因型的蛭石盒筛选

柴建芳,张翠绵,林景,马秀英,赵和,王海波

(河北省农林科学院遗传生理研究所, 河北省植物转基因中心, 石家庄 050051)

收稿日期:2015-03-26 修回日期:2015-06-23 出版日期:2015-08-15 发布日期:2015-08-15
作者简介:柴建芳|研究员|博士|主要从事小麦分子育种研究。E-mail: chai87652130@163.com
基金资助:
国家转基因生物新品种培育重大专项（2014ZX08002-005）资助。

Screening Nitrogen-Efficient Wheat Genotypes with Plastic Box Filled with Vermiculite

CHAI Jian-fang, ZHANG Cui-mian, LIN Jing, MA Xiu-ying, ZHAO He, WANG Hai-bo

(Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China) (Plant Genetic Engineering Center of Hebei Province, Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China)

Received:2015-03-26 Revised:2015-06-23 Online:2015-08-15 Published:2015-08-15

摘要/Abstract

摘要：

为探索一种简便易行的小麦氮高效基因型筛选方法，以塑料盒为栽培容器，以浇施营养液的蛭石为栽培介质，对黄淮麦区24个小麦基因型在3种氮素水平下进行了氮高效基因型的筛选。结果表明，这种蛭石盒筛选法，能有效筛选出不同氮素水平的氮高效基因型，利用该方法筛选出的低氮高效基因型有石家庄8号、邯6172和科农199，高氮高效基因型有矮抗58和092-38，氮不敏感基因型有092-39，其中石家庄8号为典型的低氮高效型品种，矮抗58为典型的高氮高效型品种，筛选结果与材料的田间表现比较一致。这种蛭石盒筛选法不但操作简单，而且筛选效果明显，可应用于小麦营养高效研究。

关键词: 小麦；蛭石；氮素营养效率；基因型筛选

Abstract:

To simplify the process for screening nitrogen efficiency of bulky wheat genotype, plastic boxes were used as cultural vessel with vermiculite and nutrient solution as cultivation medium. 24 wheat genotypes from the Huang-Huai Valley Region were evaluated under 3 nitrogen levels. The results indicated that this method with vermiculite plastic boxes was effective to screen nitrogen-efficient genotypes at different nitrogen levels. Shijiazhuang 8, Han 6172 and Kenong 199 were of high efficient genotypes under low nitrogen condition, Aikang 58 and 092-38 were of high efficient genotypes under high nitrogen condition, and 092-39 was of nitrogen insensitive genotype. Among them, Shijiazhuang 8 was a typical low nitrogen and high efficient variety, and Aikang 58 was a typical high nitrogen and high efficient variety. The screening results were in consistant with the field performances of the material. Therefore, the method of vermiculite culture in plastic box is not only operated simply, but also has obvious screening effect. It could be used in studying wheat nutrient efficiency.

Key words: wheat, vermiculite, nitrogen nutrient efficiency, genotype screening

中图分类号:

S512.1

柴建芳,张翠绵,林景,马秀英,赵和,王海波. 小麦氮高效基因型的蛭石盒筛选[J]. , 2015, 17(4): 129-134.

CHAI Jian-fang, ZHANG Cui-mian, LIN Jing, MA Xiu-ying, ZHAO He, WANG Hai-bo. Screening Nitrogen-Efficient Wheat Genotypes with Plastic Box Filled with Vermiculite[J]. , 2015, 17(4): 129-134.

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小麦氮高效基因型的蛭石盒筛选

Screening Nitrogen-Efficient Wheat Genotypes with Plastic Box Filled with Vermiculite

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