关键词: 适配体生物传感器, 赭曲霉毒素A, 血糖仪

Abstract:

Despite years of research, most current methods for quantitative detection of small molecular toxins still require laboratory-based or customized devices that are not widely available to the general public for quantitative analysis. A novel portable biosensor for sensitive and selective detection of ochratoxin A (OTA) was developed based on the specific recognition between aptamer and OTA using a personal glucose meter (PGM) as readout. The enhanced signal of the PGM had a linear relationship with the concentration of OTA in the range of 1.0×10-8～4.0×10-6 mol/L, the detection limit was 6.7×10-9 mol/L (2.69 μg/kg). This method could be used to quantitatively detect OTA present in infant rice cereal and Chinese wildrye samples with good recoveries in the range of 84%～122%. This aptasensor provided a new method to determine OTA and displayed good potential for practical application.

Key words: aptasensor, ochratoxin A(OTA), glucose meter