关键词: 三叶半夏, 丛生块茎途径, 组培快繁

Abstract: One of the most commonly used Chinese medical herb species, Pinellia, (Pinellia ternate) (Thunb.) Berit. has been used  over 1 000 a. Under natural conditions, its reproduction efficiency is quite low, and wild resources are decreased rapidly. Therefore, it becomes specially important to  artificially reproduce pinellia species to satisfy the social requirement. In order to solve the above mentioned problem, this study combined the tuber growth characteristics with the traditional tissue culture method, and obtained the ‘clusters tubers path’ of pinellia. The proliferation condition, subculture times, tissue culture variation of subculture seedling, induced isolation and cultivation matrix were investigated. The results showed that the proper culture medium was Ms+6-BA 1.0 mg/L+NAA 0.05 mg/L+sugar 30 g/L+agar 6.0 g/L. Multiplication rate of pinellia was 15 in the start subculture 22 generations after the optimization of the clusters tubers route. These tissues could be used as reproduction material after removing those variate tissue culture seedlings. The gained cluster tuber plants could be planted in sand or vermiculite. Water was cut off after 60 d and the sprouts were damping off. Big quantities of separated tubers were got after sceening out the substrate.

Key words: Pinellia ternata (Thunb.)Breit., clusters tubers path, tissue culture and rapid propagation