关键词: 马铃薯Y病毒, 病毒诱导的基因沉默, 发酵条件优化, 施用方法

Abstract: Virus-induced gene silencing (VIGS) is a widely applied natural mechanism for plant defense against virus, which can effectively prevent potato virus Y disease. Taking the control effect of VIGS expression vector fermentation broth on potato virus Y disease as detection index, this study screened the culture medium needed by fermenting the best fermentation broth, fermentation conditions and application methods. The results showed that the optimal medium was YEP (yeast extract broth peptone), optimal fermentation conditions were medium volume 60 mL/200 mL, initial pH 7.0, fermentation temperature 30℃ and shaking culture time 24 h. The optimal application methods were dilution 100 times, “leaf spraying + transplanting root irrigation”, and “mixture of VIGS biologics containing PVY CP and HC-Pro gene fragments”. The above results provided a theoretical basis for fermenting VIGS biocontrol agent, and layed a foundation for the field application.

Key words: potato virus Y, virus-induced gene silence, optimization of fermentation condition, application method