刺梨白粉病拮抗菌生长条件优化及其发酵液抑菌稳定性探究

doi:10.13304/j.nykjdb.2018.0718

中国农业科技导报 ›› 2019, Vol. 21 ›› Issue (11): 84-93.DOI: 10.13304/j.nykjdb.2018.0718

刺梨白粉病拮抗菌生长条件优化及其发酵液抑菌稳定性探究

冉继平1,2,闫岩2*,李岑1,毛玉涛3,肖玉4,冯婷婷5

1.贵州理工学院制药工程学院, 贵阳 550003; 2.贵州大学酿酒与食品工程学院, 贵州省发酵工程与生物制药重点实验室, 贵阳 550025; 3.贵阳市粮油质量检测中心, 贵阳 550025; 4.贵州大学机械工程学院, 贵阳 550025; 5.贵州省产品质量监督检验院, 贵阳 550004

收稿日期:2018-11-28 出版日期:2019-11-15 发布日期:2018-12-21
通讯作者: *通信作者闫岩 E-mail: yandouble@126.com
作者简介:冉继平 E-mail：1251697119@qq.com;
基金资助:
贵州省科技合作项目（黔科合LH字\[2015\]7099）；贵州省科技计划项目（黔科合支撑\[2016\]2618）。

Growth Conditions Optimization of Antagonistic Bacteria Against Powdery Mildew of Rosa roxburghii Tratt and Its Fermentation Broth Antibacterial Stability

RAN Jiping1,2, YAN Yan2*, LI Cen 1, MAO Yutao3, XIAO Yu4, FENG Tingting5

1.College of Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, China; 2.The Provincial Key Laboratory of Fermentation Engineering and Biopharmaceutics, School of Food and Wine Engineering, Guizhou University, Guiyang 550025, China; 3.Oil Quality Testing Center of Guiyang, Guiyang 550025, China; 4.School of Mechanical Engineering, Guizhou University, Guiyang 550025, China; 5.Institution of Supervision and Inspection Product Quality of Guizhou Province, Guiyang 550004, China

Received:2018-11-28 Online:2019-11-15 Published:2018-12-21

摘要/Abstract

摘要： 为找到防治刺梨白粉病的拮抗菌培养液，经过刺梨叶片离体防治试验表明拮抗菌浓度与白粉病防治效果成正相关，采用三因素三水平响应面法优化拮抗菌JK-6的生长条件。同时探究温度、pH、紫外光、蛋白酶以及不同贮藏时间对JK-6发酵液抑制白粉病稳定性的影响。结果表明：JK-6的最佳培养条件组合为葡萄糖2 g/mL、蛋白胨1 g/mL、NaCl浓度0.8%、温度33.6 ℃、pH 7.6、装液量40 mL、摇床转速160 r/min，在该条件下拮抗菌JK-6的发酵液OD600为1.418；发酵液温度高于40 ℃、pH为酸性和碱性时，JK-6发酵液对白粉病孢子萌发的抑制效果均降低，发酵液对紫外光和蛋白酶的耐受能力较高，在常温下保藏1个月仍然具有较高的抑菌活性。JK-6对刺梨白粉病具有一定的防治效果。

关键词: 拮抗菌, 生长条件, 发酵液, 抑菌, 稳定性

Abstract: In order to research the optimal culture solution of antagonistic bacteria against the Rosa roxburghii powdery mildew, this study carried out in vitro control experiment on leaves of R. roxburghii. The results showed that the concentration of antagonistic bacteria was positively correlated with the control effect of powdery mildew. The growth conditions of antagonistic bacteria JK-6 were optimized by 3-factor and 3-level response surface methodology. Meanwhile, the effects of temperature, pH, ultraviolet light, protease and different storage period on the stability of JK-6 fermentation broth for inhibiting powdery mildew were investigated. The results showed that the optimal medium formula of JK-6 was glucose 2 g/mL, peptone 1 g/mL, and NaCl concentration 0.8%; the best culture conditions was temperature 33.6 ℃, pH 7.6, liquid volume 40 mL, shaker speed 160 r/min. Under these conditions, the OD600 of the antagonistic bacteria JK-6 fermentation broth was 1.418. When the fermentation temperature was over 40 ℃, and the pH was acidity and alkalinity, the inhibitory effect of JK-6 fermentation broth on germination of powdery mildew spores decreased. The tolerance of fermentation broth to ultraviolet light and protease were higher, and under room temperature for one month, the fermentation broth still had high antibacterial activity. The present research suggested that JK-6 could significantly inhibit R. roxburghii powdery mildew and had certain guiding significance for the prevention and control of R. roxburghii powdery mildew.

Key words: antagonistic bacteria, growth conditions, fermentation broth, antibacterial, stability

冉继平1,2,闫岩2*,李岑1,毛玉涛3,肖玉4,冯婷婷5. 刺梨白粉病拮抗菌生长条件优化及其发酵液抑菌稳定性探究[J]. 中国农业科技导报, 2019, 21(11): 84-93.

RAN Jiping1,2, YAN Yan2*, LI Cen 1, MAO Yutao3, XIAO Yu4, FENG Tingting5. Growth Conditions Optimization of Antagonistic Bacteria Against Powdery Mildew of Rosa roxburghii Tratt and Its Fermentation Broth Antibacterial Stability[J]. Journal of Agricultural Science and Technology, 2019, 21(11): 84-93.

[1]	杨华1,李江2,张维1,周正富1,燕永亮1,郭嘉3,刘相国3,郝东云3,林敏1,柯秀彬1*. 施氏假单胞菌在玉米根际的固氮效率和促生效果研究[J]. 中国农业科技导报, 2021, 23(4): 76-84.
[2]	周茂超1,2,黄艳娜2,段赛菲1,2,束仕元1,2,唐雪明2*. 微生物种衣剂的研制及其对玉米苗期生长的影响[J]. 中国农业科技导报, 2021, 23(4): 110-118.
[3]	胡旭凯,陈居田,朱利霞,李俐俐. 干湿交替对土壤团聚体特征的影响[J]. 中国农业科技导报, 2021, 23(2): 141-149.
[4]	欧爱群1,2,郭娜娜2,刘富海3,刘然3,彭文君2,李江红1*. 蜂蜜成熟过程中主要成分和抑菌特性变化研究[J]. 中国农业科技导报, 2020, 22(2): 101-106.
[5]	刘洋,冉聪,游桂香,何婧芝,吴卫,侯凯*. 川黄柏中盐酸小檗碱HPLC测定优化及其抑菌活性评价[J]. 中国农业科技导报, 2020, 22(2): 179-186.
[6]	梁艳琼,李锐,吴伟怀,习金根,谭施北,郑金龙,黄兴,陆英,贺春萍,易克贤. Bacillus subtilis Czk1挥发性化合物对橡胶树红根病菌的抑制作用[J]. 中国农业科技导报, 2020, 22(11): 116-123.
[7]	王薇,李泳,王伟,边雪,李熙英*. Y-S-Y12发酵液和生物质热解液混配对辣椒炭疽病的防治及作用机理[J]. 中国农业科技导报, 2020, 22(10): 129-138.
[8]	王楠1,李刚强1,李云龙2,李永斌2,张浩玮2,王民洋2,王莉瑛2,刘德虎1,陈三凤2*. 固氮类芽孢杆菌的分离鉴定及其促生、抑菌活性的测定[J]. 中国农业科技导报, 2019, 21(5): 95-103.
[9]	陈永对1,朱海滨2,胡占军2,杨义2,吴阔1,董家红1,张仲凯1. 五种芳香植物精油对烟草疫霉的抑菌活性分析[J]. 中国农业科技导报, 2019, 21(5): 104-109.
[10]	林杰1,张阳1,朱艳芳1,董波1,潘颖1,丁鸣鸣2. 淮北土石山区不同土地利用方式对土壤团聚体组成的影响[J]. 中国农业科技导报, 2019, 21(4): 133-142.
[11]	魏晨阳,彭晴,乔宇,徐小轻,张宇微,谢静静,王凯强,田丹丹,张鹏博,石波*. 表乳糖对长双歧杆菌抑制产气荚膜梭菌生长的影响[J]. 中国农业科技导报, 2019, 21(3): 54-61.
[12]	王东方,王庆忠,高明刚. 乳浆大戟提取物的杀虫抑菌活性研究[J]. 中国农业科技导报, 2018, 20(8): 128-133.
[13]	刘慧颖1,2,乔宇1,石波1,彭晴1*. 不同形貌氧化锌微/纳米颗粒对食源性致病菌的抑菌研究[J]. 中国农业科技导报, 2018, 20(5): 140-147.
[14]	高原,王福玲,贾琦,刘微,周艳岩,李佳丽. 蒲公英内生真菌的分离、鉴定及抑菌活性研究[J]. 中国农业科技导报, 2017, 19(6): 55-60.
[15]	李晓娇,晏爱芬,杨丽华,何健民,侯洪波*. 龙陵铁皮石斛花色苷的提取工艺及抑菌活性研究[J]. 中国农业科技导报, 2017, 19(4): 119-127.

刺梨白粉病拮抗菌生长条件优化及其发酵液抑菌稳定性探究

Growth Conditions Optimization of Antagonistic Bacteria Against Powdery Mildew of Rosa roxburghii Tratt and Its Fermentation Broth Antibacterial Stability

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics