关键词: 黄柏结晶物, 盐酸小檗碱, 高效液相色谱, 抑菌活性

Abstract: To establish an optimized method for the determination of berberine hydrochloride in the crystals of Phellodendron chinensis by high performance liquid chromatography (HPLC), and to evaluate the inhibitory activity of P. chinense against drug-resistant bacteria, a new method was determined by optimizing the mobile phase and the detection conditions, and the antibacterial activity of the drug-resistant strain was evaluated by the antibacterial test in vitro. The results showed that the mobile phase was consisted of acetonitrile-0.1% phosphoric acid solution (50∶50) (0.1 g sodium dodecyl sulfate per 100 mL phosphoric acid solution), the detection wavelength was 265 nm, the flow rate was 1 mL·min-1, column temperature was 30 ℃. When the concentration of berberine hydrochloride was between 103 mg·L-1 and 515 mg·L-1, there was a good linear relationship between berberine and their corresponding peak area integral values (R2 ≤ 0.999 7). Under these conditions, the precision, stability and repeatability of the determination process were tested with RSD of 0.49%, 1.23% and 0.29%, respectively, which indicated that the test method was accurate. The average recovery rate was 97.84%～98.06%, and the RSD was 0.44%～1.00%, which indicated that the method was accurate and effective. The bacteriostatic activity test showed that the crystals of P. chinense had a good inhibitory effect on drug-resistant Staphylococcus aureus and Pseudomonas aeruginosa. The optimized method was safe, rapid, accurate and effective, which could be used for analysis and determination of berberine hydrochloride in P. chinensis, and berberine hydrochloride had a good bacteriostatic effect. The research broadened the types of natural products of resistant bacteria and provided a basis for clinical and research of berberine hydrochloride.

Key words: Phellodendron chinensis crystal, berberine hydrochloride, high performance liquid chromatography, bacteriostasis activity