关键词: 高效液相色谱法, 鼠尾草酸, 鼠尾草酚, 迷迭香酸

Abstract: In order to optimize process of simultaneous determination of carnosic acid, carnosol and rosmarinic acid, the detection wavelength, precision, stability, repeatability and recovery rate of HPLC method were screened and optimized. The recovery rates of rosmarinic acid, carnosol and carnosic acid were 86.4%, 85.6% and 88.8%, respectively, with the correlation coefficient above 0.999 in the concentration range of 5~100 mg·L^-1. After testing it showed that the contents of carnosic acid (25 585 mg·kg^-1) and carnosol (11 823 mg·kg^-1) in the leaf of Rosmarinus officinalis "Pine"were the highest. The content of rosmarinic acid in the leaf of Rosmarinus officinalis "Rex" was the highest (7 572.67 mg·kg^-1). The highest contents of carnosic acid (1 570.00 mg·kg^-1), carnosol (1 603.33 mg·kg^-1) and rosmarinic acid (1 304.67 mg·kg^-1) were determinated in the stems of Rosmarinus officinalis L. among the four varieties. HPLC method was stable, reliable and efficient after optimization, which  provided a basis for the development and utilization of non-volatile components of rosemary.

Key words: HPLC, carnosic acid, carnosol, rosmarinic acid