关键词: 作物;叶酸衍生物;微生物法;高效液相色谱法

Abstract:

Folic acid is a water-soluble vitamin and an extremely important co-factor that can mediate one-carbon unit transfer. Human cannot synthesize folate de novo, and the folate content in crops is usually quite low, especially in cereal grains, thus causing a global problem of folate deficiency in human. At present, people are improving the folate content in crops through biofortification. How to obtain reliable data about the content of folate derivatives in crops is very important. This paper provides a brief overview of folate extraction and various methodologies for folate analysis, with focus on microbiological method and high performance liquid chromatography (HPLC) concerning detection method establishment, technical principles and applications.

Key words: crop, folate derivatives, microbiological method, high performance liquid chromatography