不同形貌纳米氢氧化镁对芒果叶斑病原真菌的抑制作用

doi:10.13304/j.nykjdb.2021.0977

中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (3): 140-147.DOI: 10.13304/j.nykjdb.2021.0977

• 动植物健康 • 上一篇

不同形貌纳米氢氧化镁对芒果叶斑病原真菌的抑制作用

张顶洋(), 邱赛飞, 饶文华, 郭雪萍, 曹芳, 翟天鋆, 关雄(), 潘晓鸿()

福建农林大学植物保护学院，闽台作物有害生物生态防控国家重点实验室，生物农药与化学生物学教育部重点实验室，福州 350002

收稿日期:2021-11-17 接受日期:2021-12-29 出版日期:2022-03-15 发布日期:2022-03-14
通讯作者: 关雄,潘晓鸿
作者简介:张顶洋 Email： 15071844424@163.com
基金资助:
福建省科技厅面上项目(2020J01522);福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A03);福建农林大学科技发展资金项目(CXZX2019005S);福建农林大学优秀硕士学位论文资助基金项目(1122YS01006)

Inhibitory Effect of Nano-Mg（OH）₂ with Different Morphology on Pathogen Mango Phoma Leaf Spot

Dingyang ZHANG(), Saifei QIU, Wenhua RAO, Xueping GUO, Fang CAO, Tianyun ZHAI, Xiong GUAN(), Xiaohong PAN()

Key Lab of Biopesticide and Chemical Biology，Ministry of Education； State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops，College of Plant Protection，Fujian Agriculture and Forestry University，Fuzhou 350002，China

Received:2021-11-17 Accepted:2021-12-29 Online:2022-03-15 Published:2022-03-14
Contact: Xiong GUAN,Xiaohong PAN

摘要/Abstract

摘要：

叶斑病是芒果重要的叶部病害，造成芒果产量和质量下降。纳米氢氧化镁制备简单，环境友好且抗菌谱广，被广泛地应用于抗菌领域。对芒果叶斑病病原真菌进行分离纯化，通过形态学观测和ITS-rDNA序列分析，确定分离的菌株为芒果拟茎点霉叶斑病病原菌，属于间作壳属（Diaporthe musigena）。合成制备了3种不同形貌的纳米氢氧化镁，分别命名为MHNPs-MgO600、MHNPs-MgO80和MHNPs-MgCl₂，利用X-射线粉末衍射（XRD）与谢乐公式计算3种纳米颗粒在（101）面的尺寸，分别为60.50、11.63和13.52 nm。通过扫描电镜、比表面积分析仪和Zeta电位分析仪对3种纳米氢氧化镁进行表征，它们分别为规则的片状、花瓣状和六边形，且在比表面积、表面电荷等特性上存在很大的差异。采用平板涂布法计算纳米氢氧化镁对病原菌的抑制率，发现3种纳米氢氧化镁均能有效抑制芒果叶斑病病原菌的生长，比表面积最大、Zeta电位最小的MHNPs-MgO80抑制效果最佳，且随着氢氧化镁质量浓度的增加抑制效果更为明显。比较了不同纳米氢氧化镁对芒果叶斑病病原菌的抑制作用，发现比表面积和Zeta电位是影响抑菌效果的主要因素。研究结果为选择高效环保纳米氢氧化镁抗真菌制剂提供相关的科学依据和技术支撑。

关键词: 纳米氢氧化镁, 芒果叶斑病, 抗菌

Abstract:

Mango phoma leaf spot is a severe leaf disease that caused yield and quality reduction. Nano-Mg（OH）₂ is widely used in antibacterial field because of simple preparation， environmental friendliness， and wide antibacterial spectrum. Through morphological observation and sequence analysis of ITS-rDNA as well as phylogenetic comparison， the isolated pathogen was identified as mango phoma leaf spot which belonged to Diaporthe musigena. In this study， 3 different morphologies nano-Mg（OH）₂ was synthesized and named as MHNPs-MgO600， MHNPs-MgO80， and MHNPs-MgCl₂， respectively. X-ray powder diffraction （XRD） and Scherrer equation were used to calculate the sizes of nano-Mg（OH）₂ at （101） direction， which were 60.50， 11.63， and 13.52 nm， respectively. They were characterized by scanning electron microscopy， specific surface area analyzer， and Zeta potential analyzer， and the results showed that the 3 kinds of nano-magnesium hydroxide were regular flakes， petals， and hexagons， and there were great differences in specific surface area and surface charge. The inhibition rate of nano-Mg（OH）₂ against pathogen was calculated by plate coating method， the result suggested that 3 types of nano-Mg（OH）₂ had inhibiting effect， and the MHNPs-MgO80 manifested the best inhibition effect owing to its maximum specific surface area and minimum potential. Furthermore， with an increase in concentration， the inhibiting effect was more obvious. This study compared the inhibitory effect of different nano-Mg（OH）₂ on pathgen of mango phoma leaf spot， with specific surface area and Zeta potential as the factors affecting the inhibition effect of nano-Mg（OH）₂ on pathogenic bacteria. The findings of this study provide scientific basis and technical support for the selection of efficient and environmentally friendly nano magnesium hydroxide antifungal agents.

Key words: Nano-Mg（OH）₂, mango phoma leaf spot, antifungal effect

中图分类号:

S436.6

张顶洋, 邱赛飞, 饶文华, 郭雪萍, 曹芳, 翟天鋆, 关雄, 潘晓鸿. 不同形貌纳米氢氧化镁对芒果叶斑病原真菌的抑制作用[J]. 中国农业科技导报, 2022, 24(3): 140-147.

Dingyang ZHANG, Saifei QIU, Wenhua RAO, Xueping GUO, Fang CAO, Tianyun ZHAI, Xiong GUAN, Xiaohong PAN. Inhibitory Effect of Nano-Mg（OH）₂ with Different Morphology on Pathogen Mango Phoma Leaf Spot[J]. Journal of Agricultural Science and Technology, 2022, 24(3): 140-147.

图/表 6

图1 病原菌形态A：病原菌显微结构；B：病原菌菌丝（红色箭头）

Fig.1 Morphology of pathogenA：Microscope structure of pathogen； B： Mycelia photograph （red arrow）

图2基于ITS序列的NJ?系统发育树

Fig. 2 NJ?system phylogenetic tree based on ITS sequence

图3 不同方法制备的纳米氢氧化镁的XRD和SEM图

Fig. 3 XRD and SEM images of nano?Mg（OH）2 prepared by different methods

图4 不同方法制备的纳米氢氧化镁的比表面积和Zeta电位

Fig. 4 BET surface area and Zeta potential of nano?Mg（OH）2 prepared by different methods

图5 纳米氢氧化氢处理后芒果叶斑病原菌生长情况

Fig. 5 Pathogen growth of mango phoma leaf spot treated with different nano?Mg（OH）2

表1 不同质量浓度的纳米氢氧化镁对芒果叶斑病原菌的抑菌率

Table 1 Inhibition rate of pathogen of mango phoma leaf spot at different concentrations for three nano?Mg（OH）2

样品/ Simple	抑制率 Inhibition rate/%
样品/ Simple	T1	T2	T3
MHNPs-MgO600	6.67±2.44 aα	45.19±2.57 aβ	50.37±0.50 aβ
MHNPs-MgO80	7.41±1.13 aα	48.89±1.88 aβ	71.11±1.58 cγ
MHNPs- MgCl₂	0.74±1.28 bα	41.48±3.59 bβ	53.33±2.18 bγ

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不同形貌纳米氢氧化镁对芒果叶斑病原真菌的抑制作用

Inhibitory Effect of Nano-Mg（OH）₂ with Different Morphology on Pathogen Mango Phoma Leaf Spot

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不同形貌纳米氢氧化镁对芒果叶斑病原真菌的抑制作用

Inhibitory Effect of Nano-Mg（OH）2 with Different Morphology on Pathogen Mango Phoma Leaf Spot

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