陆地棉COI家族基因鉴定及在干旱和盐胁迫下的表达分析

doi:10.13304/j.nykjdb.2021.0926

中国农业科技导报 ›› 2022, Vol. 24 ›› Issue (4): 63-74.DOI: 10.13304/j.nykjdb.2021.0926

陆地棉COI家族基因鉴定及在干旱和盐胁迫下的表达分析

李美丽¹(), 宿俊吉¹, 杨永林², 秦江鸿², 李鲜鲜¹, 杨德龙¹(), 马麒³(), 王彩香¹()

^1.甘肃农业大学生命科学技术学院，省部共建干旱生境作物学国家重点实验室，兰州 730070
^2.石河子农业科学研究院棉花研究所，新疆石河子 832000
^3.新疆农垦科学院棉花研究所，新疆石河子 832000

收稿日期:2021-11-02 接受日期:2021-12-09 出版日期:2022-04-15 发布日期:2022-04-19
通讯作者: 杨德龙,马麒,王彩香
作者简介:李美丽 E-mail： 2538268277@qq.com
基金资助:
国家自然科学基金项目(31801414);甘肃省自然科学基金项目(20JR10RA531);甘肃农业大学科技创新基金项目(GAU-KYQD-2018-32);棉花生物学国家重点实验室开放课题基金(CB2021A36)

Identification of COI Family Genes and Their Expression in Gossypium hirsutum L. Under Drought and Salt Stress

Meili LI¹(), Junji SU¹, Yonglin YANG², Jianghong QIN², Xianxian LI¹, Delong YANG¹(), Qi MA³(), Caixiang WANG¹()

^1.State Key Laboratory of Aridland Crop Science，College of Life Science and Technology，Gansu Agricultural University，Lanzhou 730070，China
^2.Cotton Research Institute，Shihezi Academy of Agricultural Sciences，Xinjiang Shihezi 832000，China
^3.Cotton Research Institute，Xinjiang Academy of Agricultural Reclamation Sciences，Xinjiang Shihezi 832000，China

Received:2021-11-02 Accepted:2021-12-09 Online:2022-04-15 Published:2022-04-19
Contact: Delong YANG,Qi MA,Caixiang WANG

摘要/Abstract

摘要：

COI1（COR-insensitive 1）与Skp1-Cullin-F-box蛋白组成茉莉素信号转导的核心组分SCF^COI1复合物，在植物生长发育和响应逆境胁迫中发挥重要作用。从全基因组水平上鉴定出48个陆地棉COI家族基因，其不均匀地分布在21条染色体上，主要定位于细胞核和细胞质中。根据系统发育关系将其分为3个亚族（Ⅰ～Ⅲ），共线性分析发现COI基因以片段复制方式在陆地棉中进行扩张。陆地棉COI基因含有1～9个外显子，其启动子区域共存在6种逆境胁迫响应元件。转录组数据显示，分别有66.7%、56.25%、68.8%和27.1%的基因在NaCl、PEG、热和冷胁迫下表达量升高，且部分基因同时响应多种非生物胁迫；qRT-PCR验证结果显示，GhCOI5-A06强烈响应NaCl和PEG胁迫，是潜在的候选基因，表明GhCOI参与非生物胁迫应答。上述结果为后续深入解析该家族基因的功能奠定基础。

关键词: 陆地棉, COI家族, 基因鉴定, 非生物胁迫

Abstract:

COI1 （COR-insensitive 1） and Skp1-Cullin-F-box protein constitute the SCF^COI1 complex，a core component of jasmine signal transduction， which plays an important role in plant growth development and response to stress. A total of 48 COI genes were identified in Gossypium hirsutum L.， which were unevenly distributed in 21 chromosomes and mainly located in the nucleus and cytoplasm. According to the phylogenetic relationship， the GhCOI genes were divided into 3 subgroups （Ⅰ~Ⅲ）. Collinearity analysis showed that COI genes expanded by segmental duplication in upland cotton. The GhCOI genes contained 1 ~ 9 exons， and there were 6 stress response elements in their promoter regions. The transcriptome data showed that 66.7%， 56.25%， 68.8% and 27.1% of genes were highly expressed under NaCl， PEG， heat and cold stress， respectively， and some genes responded to multiple abiotic stresses simultaneously. The qRT-PCR results showed that GhCOI5-A06 was a candidate gene with strong response to NaCl and PEG stress， which showed that GhCOIs involved in response to abiotic stress. Above results laid foundation for further analysis of this family genes function in cotton.

Key words: Gossypium hirsutum L., COI family, gene indentification, abiotic stress

中图分类号:

李美丽, 宿俊吉, 杨永林, 秦江鸿, 李鲜鲜, 杨德龙, 马麒, 王彩香. 陆地棉COI家族基因鉴定及在干旱和盐胁迫下的表达分析[J]. 中国农业科技导报, 2022, 24(4): 63-74.

Meili LI, Junji SU, Yonglin YANG, Jianghong QIN, Xianxian LI, Delong YANG, Qi MA, Caixiang WANG. Identification of COI Family Genes and Their Expression in Gossypium hirsutum L. Under Drought and Salt Stress[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 63-74.

图/表 9

参考文献 27

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引物名称 Primer name	正向引物 Forward primer（5’-3’）	反向引物 Reverse primer （5’-3’）
GhCOI1⁃D01	GTGACTTGACCGATGCAGGA	GAAAGCGCAAGAACCACAGG
GhCOI1⁃D06	CTCGTTTCGATGCTGTCACG	CGAGTCAAATTCCGGCAACG
GhCOI3⁃D04	CAACCCGGTATGTCCGATGT	CACCGCCTGCAAACTAGAGA
GhCOI3⁃A05	CAACCCGGTATGTCCGATGT	CACCGCCTGCAAACTAGAGA
GhCOI5⁃A05	CAGCTCTGCGTTCTGGTTTG	AGCAATCTCCTGGCAACCAT
GhCOI5⁃A06	TGGCGGAGCAAATGTCTGAT	ACTCGGAATGACCCGAAACC
GhCOI7⁃A07	CCGGCGACAGTAACAAAGGA	GGACATCCAAAGGGATCGCA
GhCOI9⁃D08	AGCTCGTAAGCCAGTTTCCC	CCTCAACGGCCAACAATTCG
GhCOI10⁃A09	TCAAGTTCCTCGCTCAACGG	TCATCTCTGCGACGCCTTTT
GhCOI10⁃D09	TTTGGCAACTGAGTGTGGGA	GCCTCCAGTGATTTGTCGGT
GhActin	ATCCTCCGTCTTGACCTTG	TGTCCGTCAGGCAACTCAT

引物名称 Primer name	正向引物 Forward primer（5’-3’）	反向引物 Reverse primer （5’-3’）
GhCOI1⁃D01	GTGACTTGACCGATGCAGGA	GAAAGCGCAAGAACCACAGG
GhCOI1⁃D06	CTCGTTTCGATGCTGTCACG	CGAGTCAAATTCCGGCAACG
GhCOI3⁃D04	CAACCCGGTATGTCCGATGT	CACCGCCTGCAAACTAGAGA
GhCOI3⁃A05	CAACCCGGTATGTCCGATGT	CACCGCCTGCAAACTAGAGA
GhCOI5⁃A05	CAGCTCTGCGTTCTGGTTTG	AGCAATCTCCTGGCAACCAT
GhCOI5⁃A06	TGGCGGAGCAAATGTCTGAT	ACTCGGAATGACCCGAAACC
GhCOI7⁃A07	CCGGCGACAGTAACAAAGGA	GGACATCCAAAGGGATCGCA
GhCOI9⁃D08	AGCTCGTAAGCCAGTTTCCC	CCTCAACGGCCAACAATTCG
GhCOI10⁃A09	TCAAGTTCCTCGCTCAACGG	TCATCTCTGCGACGCCTTTT
GhCOI10⁃D09	TTTGGCAACTGAGTGTGGGA	GCCTCCAGTGATTTGTCGGT
GhActin	ATCCTCCGTCTTGACCTTG	TGTCCGTCAGGCAACTCAT

基因名称 Gene name	基因编号 Gene ID	氨基酸数目 Number of amino acids/aa	分子量 Molecular weight/kD	等电点pI	亚细胞定位 Subcellular localization
GhCOI1⁃A01	GH_A01G1190	534	57.36	8.7	叶绿体、细胞核Chloroplast， nucleus
GhCOI1⁃D01	GH_D01G1258	534	57.54	8.82	细胞核Nucleus
GhCOI1⁃A05	GH_A05G2065	534	57.38	8.86	细胞核Nucleus
GhCOI1⁃D05	GH_D05G2095	535	57.44	8.83	细胞核Nucleus
GhCOI1⁃A06	GH_A06G0210	528	56.82	8.74	叶绿体、线粒体Chloroplast， mitochondria
GhCOI1⁃D06	GH_D06G0195	528	56.7	8.86	叶绿体、线粒体Chloroplast， mitochondria
GhCOI2⁃A02	GH_A02G1803	544	58.97	8.49	细胞核Nucleus
GhCOI2⁃D03	GH_D03G0256	514	55.87	7.87	细胞质Cytoplasm
GhCOI2⁃A10	GH_A10G0913	545	59.31	8.6	细胞质Cytoplasm
GhCOI2⁃D10	GH_D10G1048	545	59.21	8.59	细胞质Cytoplasm
GhCOI3⁃D04	GH_D04G0696	591	66.99	5.98	细胞质Cytoplasm
GhCOI3⁃A05	GH_A05G3439	589	66.74	5.81	细胞质Cytoplasm
GhCOI4⁃A05	GH_A05G0481	362	39.99	6.32	细胞质Cytoplasm
GhCOI4⁃D05	GH_D05G0480	362	40.08	6.96	细胞质Cytoplasm
GhCOI5⁃A05	GH_A05G2217	611	68.38	5.97	细胞核Nucleus
GhCOI5⁃D05	GH_D05G2242	611	68.32	5.88	叶绿体Chloroplast
GhCOI5⁃A06	GH_A06G0343	615	69.03	5.67	细胞核Nucleus
GhCOI5⁃D06	GH_D06G0328	615	69.11	5.54	细胞核Nucleus
GhCOI6⁃A06	GH_A06G1509	701	78.94	6.57	叶绿体 Chloroplast
GhCOI6⁃D06	GH_D06G1549	428	47.46	8.17	细胞质Cytoplasm
GhCOI6⁃A10	GH_A10G0382	650	72.69	6.75	叶绿体Chloroplast
GhCOI6⁃D10	GH_D10G0398	650	72.59	6.75	细胞核Nucleus
GhCOI6⁃A12	GH_A12G1052	702	79.13	5.83	细胞核Nucleus
GhCOI6⁃D12	GH_D12G1027	702	79.06	5.95	细胞核Nucleus
GhCOI7⁃A07	GH_A07G2611	579	65.23	7.93	细胞质、细胞核Cytoplasm， nucleus
GhCOI7⁃D07	GH_D07G2552	572	64.35	8.23	细胞核Nucleus

基因名称 Gene name	基因编号 Gene ID	氨基酸数目 Number of amino acids/aa	分子量 Molecular weight/kD	等电点pI	亚细胞定位 Subcellular localization
GhCOI1⁃A01	GH_A01G1190	534	57.36	8.7	叶绿体、细胞核Chloroplast， nucleus
GhCOI1⁃D01	GH_D01G1258	534	57.54	8.82	细胞核Nucleus
GhCOI1⁃A05	GH_A05G2065	534	57.38	8.86	细胞核Nucleus
GhCOI1⁃D05	GH_D05G2095	535	57.44	8.83	细胞核Nucleus
GhCOI1⁃A06	GH_A06G0210	528	56.82	8.74	叶绿体、线粒体Chloroplast， mitochondria
GhCOI1⁃D06	GH_D06G0195	528	56.7	8.86	叶绿体、线粒体Chloroplast， mitochondria
GhCOI2⁃A02	GH_A02G1803	544	58.97	8.49	细胞核Nucleus
GhCOI2⁃D03	GH_D03G0256	514	55.87	7.87	细胞质Cytoplasm
GhCOI2⁃A10	GH_A10G0913	545	59.31	8.6	细胞质Cytoplasm
GhCOI2⁃D10	GH_D10G1048	545	59.21	8.59	细胞质Cytoplasm
GhCOI3⁃D04	GH_D04G0696	591	66.99	5.98	细胞质Cytoplasm
GhCOI3⁃A05	GH_A05G3439	589	66.74	5.81	细胞质Cytoplasm
GhCOI4⁃A05	GH_A05G0481	362	39.99	6.32	细胞质Cytoplasm
GhCOI4⁃D05	GH_D05G0480	362	40.08	6.96	细胞质Cytoplasm
GhCOI5⁃A05	GH_A05G2217	611	68.38	5.97	细胞核Nucleus
GhCOI5⁃D05	GH_D05G2242	611	68.32	5.88	叶绿体Chloroplast
GhCOI5⁃A06	GH_A06G0343	615	69.03	5.67	细胞核Nucleus
GhCOI5⁃D06	GH_D06G0328	615	69.11	5.54	细胞核Nucleus
GhCOI6⁃A06	GH_A06G1509	701	78.94	6.57	叶绿体 Chloroplast
GhCOI6⁃D06	GH_D06G1549	428	47.46	8.17	细胞质Cytoplasm
GhCOI6⁃A10	GH_A10G0382	650	72.69	6.75	叶绿体Chloroplast
GhCOI6⁃D10	GH_D10G0398	650	72.59	6.75	细胞核Nucleus
GhCOI6⁃A12	GH_A12G1052	702	79.13	5.83	细胞核Nucleus
GhCOI6⁃D12	GH_D12G1027	702	79.06	5.95	细胞核Nucleus
GhCOI7⁃A07	GH_A07G2611	579	65.23	7.93	细胞质、细胞核Cytoplasm， nucleus
GhCOI7⁃D07	GH_D07G2552	572	64.35	8.23	细胞核Nucleus

基因名称 Gene name	基因编号 Gene ID	氨基酸数目 Number of amino acids/aa	分子量 Molecular weight/kD	等电点pI	亚细胞定位 Subcellular localization
GhCOI7⁃A11	GH_A11G0655	572	64.46	7.42	细胞核Nucleus
GhCOI7⁃D11	GH_D11G0684	572	64.28	8.2	细胞核Nucleus
GhCOI8A⁃A08	GH_A08G0483	587	65.9	5.8	细胞核Nucleus
GhCOI8B⁃A08	GH_A08G0792	586	65.92	6.13	细胞核Nucleus
GhCOI8C⁃A08	GH_A08G1389	586	65.62	5.5	叶绿体、细胞质Chloroplast， cytoplasm
GhCOI8A⁃D08	GH_D08G0497	587	65.67	5.61	细胞核Nucleus
GhCOI8B⁃D08	GH_D08G0784	586	65.91	5.97	细胞质Cytoplasm
GhCOI8C⁃D08	GH_D08G1411	586	65.57	5.51	叶绿体Chloroplast
GhCOI8⁃A11	GH_A11G1227	585	65.64	6.54	细胞质Cytoplasm
GhCOI8⁃D11	GH_D11G1257	585	65.49	5.76	细胞质、细胞核Cytoplasm， nucleus
GhCOI9⁃A08	GH_A08G2517	467	52.62	7.74	细胞核Nucleus
GhCOI9⁃D08	GH_D08G2524	467	52.6	6.91	细胞质Cytoplasm
GhCOI9A⁃A11	GH_A11G1519	395	43.46	6.63	细胞质Cytoplasm
GhCOI9B⁃A11	GH_A11G3019	654	72.04	8.25	叶绿体、细胞核、细胞质Chloroplast， nucleus， cytoplasm
GhCOI9A⁃D11	GH_D11G1550	395	43.43	6.98	细胞质Cytoplasm
GhCOI9B⁃D11	GH_D11G3049	654	72.27	8.17	细胞核Nucleus
GhCOI10⁃A09	GH_A09G2525	592	64.35	7.91	细胞质Cytoplasm
GhCOI10⁃D09	GH_D09G2461	607	65.93	8.11	细胞质Cytoplasm
GhCOI10⁃A12	GH_A12G1492	411	44.68	5.8	细胞质、细胞核Cytoplasm， nucleus
GhCOI10⁃D12	GH_D12G1505	411	44.61	5.95	细胞质、细胞核Cytoplasm， nucleus
GhCOI11⁃A10	GH_A10G0227	586	65.71	5.97	细胞核Nucleus
GhCOI11⁃D10	GH_D10G0239	586	65.87	5.92	细胞核Nucleus

陆地棉COI家族基因鉴定及在干旱和盐胁迫下的表达分析

Identification of COI Family Genes and Their Expression in Gossypium hirsutum L. Under Drought and Salt Stress

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[1]	默韶京, 王志城, 王省芬, 刘正文, 吴立强, 张桂寅, 马峙英, 张艳, 段会军. 陆地棉GELP家族基因鉴定及其响应胁迫的表达分析[J]. 中国农业科技导报, 2022, 24(2): 93-103.
[2]	王琴琴, 陈修贵, 陆许可, 王帅, 张悦新, 范亚朋, 陈全家, 叶武威. 陆地棉GhPKE1的生物信息学分析及功能验证[J]. 中国农业科技导报, 2022, 24(1): 38-45.
[3]	李生梅, 杨涛, 黄雅婕, 任丹, 耿世伟, 李典鹏, 芮存, 高文伟. 海陆回交群体主要农艺性状与纤维品质关系的探讨[J]. 中国农业科技导报, 2021, 23(8): 16-24.
[4]	崔江慧§,杨溥原§,常金华. 高粱GRF*基因家族鉴定及在非生物胁迫下的表达分析[J]. 中国农业科技导报, 2021, 23(4): 37-46.
[5]	陈斌, 石荣康, 王志城, 刘松, 李青, 刘正文, 孙正文, 王国宁, 吴金华, 马峙英, 张艳, 王省芬. 陆地棉核心种质抗黄萎病鉴定与优异种质筛选[J]. 中国农业科技导报, 2021, 23(10): 45-51.
[6]	李子玮1,陈思蒙1,王发展1,张豪洋1,张莉2,许自成1. 硫化氢在植物中抵御非生物胁迫机制的研究进展[J]. 中国农业科技导报, 2020, 22(4): 24-32.
[7]	陈思蒙,李子玮,张璐翔,魏艳秋,朱智威,张环纬,许自成,黄五星,邵惠芳*. 硒在植物抵御胁迫中作用的研究进展[J]. 中国农业科技导报, 2020, 22(3): 6-13.
[8]	周秒依,任雯,赵冰兵,李韩帅,刘亚*. 植物MAPK级联途径应答的非生物胁迫研究进展[J]. 中国农业科技导报, 2020, 22(2): 22-29.
[9]	梁桂红,华营鹏,周婷,宋海星,张振华*. 植物液泡膜H+-ATPase和H+-PPase研究进展[J]. 中国农业科技导报, 2020, 22(1): 19-27.
[10]	韩佳慧1,刘盈盈1,江世杰2,陈云1,耿秀秀1,2,平淑珍1,王劲1,2*. 非生物胁迫下戈壁异常球菌LEA3蛋白Dgl3抗逆功能研究[J]. 中国农业科技导报, 2019, 21(9): 69-76.
[11]	王俊铎1,曾辉2,龚照龙1,梁亚军1,艾先涛1,郭江平1,莫明1,李雪源1,郑巨云1*. 陆地棉品种资源耐复合盐碱性综合评价分析[J]. 中国农业科技导报, 2019, 21(10): 1-11.
[12]	杨笑敏,芮存,张悦新,王德龙,王俊娟,陆许可,陈修贵,郭丽雪,王帅,陈超,叶武威*. 棉花DNA甲基转移酶GhDMT9抗逆性分析[J]. 中国农业科技导报, 2019, 21(10): 12-19.
[13]	王昭玉1,吕婷婷1,李爱芹2,王頔1,张子玉1,闫瑞娴1,. 陆地棉钾吸收相关基因GhHAK1的克隆与功能分析[J]. 中国农业科技导报, 2017, 19(10): 21-27.
[14]	高丽华1,刘博欣1，2,李金博3,吴燕民1,唐益雄1*. 陆地棉bHLH转录因子GhMYC4基因的克隆及功能分析[J]. 中国农业科技导报, 2016, 18(5): 33-41.
[15]	谢政文1,王连军2,陈锦洋1,王娇1,苏一钧1,杨新笋2,曹清河1*. 植物WRKY转录因子及其生物学功能研究进展[J]. 中国农业科技导报, 2016, 18(3): 46-54.