糙皮侧耳菌丝体液体发酵制备维生素D2条件的优化

doi:10.13304/j.nykjdb.2024.0014

中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (5): 231-238.DOI: 10.13304/j.nykjdb.2024.0014

糙皮侧耳菌丝体液体发酵制备维生素D₂条件的优化

何孟欣(), 彭晴, 徐小轻, 田丹丹, 弓雨欣, 熊小艳, 石波, 乔宇()

中国农业科学院饲料研究所，北京 100081

收稿日期:2024-01-08 接受日期:2024-03-04 出版日期:2025-05-15 发布日期:2025-05-20
通讯作者: 乔宇
作者简介:何孟欣 E-mail：hemengxin1998@163.com；
基金资助:
国家自然科学基金项目(32072773)

Optimization of Preparation for Vitamin D₂ by Liquid Fermentation of Pleutotus ostreatus Mycelium

Mengxin HE(), Qing PENG, Xiaoqing XU, Dandan TIAN, Yuxin GONG, Xiaoyan XIONG, Bo SHI, Yu QIAO()

Feed Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2024-01-08 Accepted:2024-03-04 Online:2025-05-15 Published:2025-05-20
Contact: Yu QIAO

摘要/Abstract

摘要：

为了从糙皮侧耳（俗称平菇，Pleutotus ostreatus）菌丝体中获取更多的维生素D₂（vitamin D₂，VD₂），利用平菇菌丝体的液体发酵和光化学转化的方法生成VD₂，并对过程条件进行优化。首先，通过优化液体发酵条件从平菇菌丝体中获得麦角甾醇；其次，对液体发酵的平菇菌丝体进行紫外线-B（ultraviolet-B，UV-B，280~320 nm）照射，分析不同紫外强度、照射面积和照射时间对VD₂形成的影响。结果表明，在以葡萄糖为碳源、以牛肉浸粉为氮源、接种量5%、振荡速度120 r·min^-1、发酵温度28 ℃、发酵5 d的条件下可获得平菇菌丝体的生物量为3.23 g DW·L^-1，麦角甾醇的产量最高可达6.40 mg·L^-1。当紫外强度为131.27 W·m^-2、照射面积为314.16 cm²、照射时间为80 min时，VD₂转化率为1.74%，VD₂产量可达111.24 μg·L^-1，比优化前提高37.55%。以上研究结果为新型食用菌生产VD₂工艺的开发提供了数据支撑。

关键词: 糙皮侧耳, 菌丝体, UV-B, 麦角甾醇, VD₂

Abstract:

In order to obtain more vitamin D₂ （VD₂） from Pleutotus ostreatus mycelium， VD₂ was produced by liquid fermentation and photochemical transformation from Pleutotus ostreatus mycelium， and the process conditions were optimized. First， more ergosterol production was obtained from P. ostreatus mycelium by optimizing the liquid fermentation conditions. Then， Ultraviolet-B （UV-B， 280~320 nm） irradiation was performed on the liquid fermentation of P. ostreatus mycelium. The effects of different UV intensity， irradiation area and irradiation time on the formation of VD₂ were analyzed. The results revealed that the mycelium biomass was 3.23 g DW·L^-1 and the yield of ergosterol reached 6.40 mg·L^-1 under the fermentation temperature 28 ℃， the inoculation amount 5%， the shaker rate 120 r·min^-1， glucose as carbon source， beef powder as nitrogen source， fermentation 5 d. When the UV intensity was 131.27 W·m^-2， the irradiation area was 314.16 cm²， and the irradiation time was 80 min， the transformation rate of VD₂ was 1.74% and the yield of VD₂ was 111.24 μg·L^-1， which the yield of VD₂ increased by 37.55% compared with the before optimization. Above results provided data support for the development of VD₂ process for the production of edible fungi.

Key words: Pleutotus ostreatus, mycelium, UV-B, ergosterol, VD₂

中图分类号:

S646.1+41

何孟欣, 彭晴, 徐小轻, 田丹丹, 弓雨欣, 熊小艳, 石波, 乔宇. 糙皮侧耳菌丝体液体发酵制备维生素D₂条件的优化[J]. 中国农业科技导报, 2025, 27(5): 231-238.

Mengxin HE, Qing PENG, Xiaoqing XU, Dandan TIAN, Yuxin GONG, Xiaoyan XIONG, Bo SHI, Yu QIAO. Optimization of Preparation for Vitamin D₂ by Liquid Fermentation of Pleutotus ostreatus Mycelium[J]. Journal of Agricultural Science and Technology, 2025, 27(5): 231-238.

图/表 5

图1 不同发酵条件下平菇菌丝体的生物量注：不同小写字母表示不同处理间在P<0.05水平差异显著。

Fig. 1 Mycelium biomass of Pleurotus ostreatus under different fermentation conditionsNote： Different lowercase letters indicate significant differences between different treatments at P<0.05 level.

图2 不同发酵时间的平菇菌丝体麦角甾醇含量注：不同小写字母表示不同处理间在P<0.05水平差异显著。

Fig. 2 Ergosterol content of Pleurotus ostreatus at different fermentation timesNote：Different lowercase letters indicate significant differences between different treatments at P<0.05 level.

图3 不同发酵时间平菇菌丝体的生物量和麦角甾醇产量注：同一指标不同小写字母表示不同处理间在P<0.05水平差异显著。

Fig. 3 Mycelium biomass and ergosterol yield of Pleurotus ostreatus at different culture timesNote：Different lowercase letters of same index indicate significant differences between different treatmetns at P<0.05 level.

图4 UV-B照射前后平菇菌丝体中麦角甾醇和VD2的HPLC谱图A：麦角甾醇和VD2标准品；B：UV-B照射前；C：UV-B照射后

Fig. 4 HPLC chromatograms of ergosterol and VD2 in Pleurotus ostreatus mycelium before and after UV-B irradiationA： Ergosterol and VD2 standard； B： Before UV-B irradiation； C： After UV-B irradiation

图5 不同UV-B条件下平菇菌丝体中VD2含量注：不同小写字母表示不同处理间在P<0.05水平差异显著。

Fig. 5 VD2 content in Pleurotus ostreatus mycelium under different UV-B conditionsNote：Different lowercase letters indicate significant differences between different treatments at P<0.05 level.

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糙皮侧耳菌丝体液体发酵制备维生素D₂条件的优化

Optimization of Preparation for Vitamin D₂ by Liquid Fermentation of Pleutotus ostreatus Mycelium

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