充气在几种饵料单细胞藻二级培养中的应用

›› 2008, Vol. 10 ›› Issue (2): 94-99.

充气在几种饵料单细胞藻二级培养中的应用

李锋[1] 毛玉泽[1] 于守团[2] 杜美荣[1] 叶乃好[1] 方建光[1]

[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]青岛红岛蛤原良种发展有限公司,山东青岛266114

收稿日期:2008-01-03 修回日期:2008-01-23 出版日期:2008-04-15 发布日期:2009-07-29
通讯作者: 方建光，研究员，博士生导师，主要从事海水养殖生态学的研究。
作者简介:李锋|工程师|主要从事饵料单细胞藻培养的研究。E-mail：lifeng@ysfri．ac．cn。
基金资助:
国家973项目（2006CB400608）,国家863计划项目（2006AA102414,2006AAl00304）,中国博士后科学基金资助项目（2006039313）,山东省博士后择优基金项目（200603061）和国家自然科学基金项目（40776090,40706050,40706048,30700619）资助.

Application of Air Bubbling Method on Amplified Incubation of Microalgae

LI Feng, MA0 Yu-ze| YU Shou-tuan| DU Mei-rong| YE Nai-hao| FANG Jian-guang

1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071; |2. Hongdao Clam 0riginal and Eugenic Breed Development Ltd.| Qingdao 266114, China

Received:2008-01-03 Revised:2008-01-23 Online:2008-04-15 Published:2009-07-29

摘要/Abstract

摘要：

采用实验与生产结合的方法，研究了充气条件下几种饵料单胞藻的生长特性。结果表明，充气能大幅度提高4种饵料单胞藻的生长速度。在充气条件下，新月菱形藻、金藻3011、3012和扁藻的最大培养密度分别为2．957×10^7，1．7×10^7，2．355×10^7和3．5×10^5细胞／mL，分别是对照组最大培养密度的3．2倍，6．5倍，4．1倍和5倍；实验组（充气）与对照组（不充气）在细胞数目、特定生长率（SGR）、相对生长率（RGR）和绝对生长率（AGR）等方面均有显著差异。二级充气培养的藻液可以直接扩种到大池进行生产培养，能有效延长生长时间，显著提高生产培养效率。

关键词: 单胞藻充气培养生长率

Abstract:

Growth characteristics of 4 microalgae in bubble condition were studied using experiment combined with production method. The results showed that bubble incubation could improve the growth rate of 4 microalgae dramatically. Under bubble condition, the maximum cell density of N. closterium,L galbana Parke 3011 ,L galbana Parke 3012 and P. subcordiformis were 2.957× 10^7, 1.7 × 10^7 ,2. 355 × 10^7 and 3.5× 10^6 cell per milliliter, and which was 3..2, 6.5, 4.1 and 5 times of the control group under the same conditions respectively. The experiment group showed significant difference with the control one in cell number, special growth rate （ SGR）, relative growth rate （RGR） and absolute growth rate （AGR）. The air-charging incubation seed can be directly used for amplified culture. Air-charging incubation can effectively prolong the growth period of the algae cell, and remarkably enhance the production efficiency.

Key words: microalgae , air-charging incubation , growth rate

中图分类号:

S963.8

李锋,毛玉泽,于守团,杜美荣,叶乃好,方建光. 充气在几种饵料单细胞藻二级培养中的应用[J]. , 2008, 10(2): 94-99.

LI Feng, MA0 Yu-ze| YU Shou-tuan| DU Mei-rong| YE Nai-hao| FANG Jian-guang . Application of Air Bubbling Method on Amplified Incubation of Microalgae[J]. , 2008, 10(2): 94-99.