Abstract:

Trichoderma reesei represents a model system to study the fungal cellulose degradation and produces profuse quantities of cellulase. The precise nature of true “inducer” and the regulation of cellulase formation by T. reesei is still insufficiently understood. The results of preliminary studies have shown the important role of β-glucosidase in the rapid induction of cellulase complex. The measurement results of Trichoderma reesei genome sequence indicates that 7 extracellular and intracellular β\|glucosidases exist. This paper reviews and analyzes the classification, structure characteristic, transcription discrepancy of β-glucosidase and its function in rapid induction of cellulase genes.

Key words: Trichoderma reesei, Cellulases, β-glucosidases, transcriptional regulation

摘要：

瑞氏木霉（Trichoderma reesei）是研究纤维素降解的主要模式生物，具有很强的分泌纤维素酶的能力，但诱导纤维素酶基因转录表达的信号分子是什么，整个纤维素酶系的表达是如何被调控的等问题至今仍没有得到明确和合理的答案。初步的研究结果显示β\|葡萄糖苷酶在纤维素酶诱导过程中具有重要作用。瑞氏木霉基因组序列测定结果表明，其胞内外存在7种β\|葡萄糖苷酶。针对瑞氏木霉中β\|葡萄糖苷酶的分类、结构特点、转录差异及其在纤维素酶快速诱导过程中的功能进行了综述和分析。

关键词: 瑞氏木霉；纤维素酶；β-葡萄糖苷酶；转录调控