Journal of Agricultural Science and Technology ›› 2021, Vol. 23 ›› Issue (1): 66-72.DOI: 10.13304/j.nykjdb.2019.0546
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LIU Qianqiu, CHEN Qiang, LEI Anmin*
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刘芊萩,陈强,雷安民*
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Abstract: With the advantages of automation, high sensitivity and precision, microfluidic technology have been used to control complex and precise biological experiments, to ensure real-time and accurate acquisition of experimental phenomena. In this paper, the applications of microfluidic devices in IVM(in vitro maturation), IVF(in vitro fertilization) and IVC(in vitro culture) of mammalian oocytes were reviewed, and the applications of microfluidic technology in mammalian embryology research were also summarized. At the same time, this paper proposed to apply the microfluidic technology to the IVF of porcine, aiming to solve and overcome the multiple spermatozoa in the IVF of porcine, and to provide a feasible solution for improving the IVF of porcine. At the same time, it provided new ideas for the optimization and improvement of human medical assisted reproductive technology.
Key words: microfluidic chip, polyspermy, assisted reproduction, in vitro fertilization, in vitro culture
摘要: 凭借自动化、高灵敏度和高精确度的优势,微流体技术可以实现复杂精细的生物学实验流程控制,保证对试验现象与试验结果的实时准确获取。通过检索近年来微流体装置在哺乳动物卵母细胞体外成熟、体外受精以及胚胎体外培养中的研究报道,总结了微流控技术在哺乳动物胚胎学研究中的应用,提出了将微流控技术应用于猪体外受精技术,目的在于解决并克服猪体外受精中存在的多精入卵,为完善猪体外受精技术提供可行的解决方案,同时为人类医学辅助生殖技术的优化完善提供新思路。
关键词: 微流控芯片, 多精入卵, 辅助生殖, 体外受精, 体外胚胎培养
LIU Qianqiu, CHEN Qiang, LEI Anmin*. Application Progress of Microfluidic Technology to Solve the Problem of Polyspermy in Porcine IVF[J]. Journal of Agricultural Science and Technology, 2021, 23(1): 66-72.
刘芊萩,陈强,雷安民*. 微流控技术在解决猪体外受精中多精入卵问题中的应用进展[J]. 中国农业科技导报, 2021, 23(1): 66-72.
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URL: https://www.nkdb.net/EN/10.13304/j.nykjdb.2019.0546
https://www.nkdb.net/EN/Y2021/V23/I1/66