Journal of Agricultural Science and Technology ›› 2021, Vol. 23 ›› Issue (10): 45-51.DOI: 10.13304/j.nykjdb.2021.0170

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Identification of Verticillium Wilt Resistance of Core Collection of Upland Cotton and Screening of Elite Resistant Germplasm

CHEN Bin, SHI Rongkang, WANG Zhicheng, LIU Song, LI Qing, LIU Zhengwen, SUN Zhengwen, WANG Guoning, WU Jinhua, MA Zhiying, ZHANG Yan*, WANG Xingfen*   

  1. State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory for Crop Germplasm Resources of 
    Hebei, College of Agronomy, Hebei Agricultural University, Hebei Baoding 071000, China
  • Received:2021-02-28 Accepted:2021-03-23 Online:2021-10-16 Published:2021-10-11

陆地棉核心种质抗黄萎病鉴定与优异种质筛选

陈斌,石荣康,王志城,刘松,李青,刘正文,孙正文,王国宁,吴金华,马峙英,张艳*,王省芬*   

  1. 河北农业大学农学院, 华北作物改良与调控国家重点实验室, 河北省作物种质资源重点实验室, 
    河北 保定 071000
  • 通讯作者: 张艳 E-mail:zhangyan7235@126.com;王省芬 E-mail:cotton@hebau.edu.cn
  • 作者简介:陈斌 E-mail:chbhebau@163.com
  • 基金资助:
    河北省科技支撑计划项目(16226307D)

Abstract: Cotton Verticillium wilt, caused by Verticillium dahliae Kleb., is a soil borne disease, which could  cause serious damage in the cotton production. The core germplasm represents the genetic diversity of the entire resource population. In this study, 419 core germplasm of upland cotton varieties (lines) were identified for Verticillium wilt resistance and selected for excellent varieties by quantitative injection of V. dahliae in hexagonal plastic bowl in an environment controlled artificial growth chamber. The results showed that there were a few resistant varieties in this 419 core collection, only 12 resistant varieties were identified, the disease index ranged from 21.67 to 25.00; 253 varieties displayed tolerant and the disease index ranged from 25.47 to 50.00, other varieties were susceptible to disease; there was no variety with immunity and high Verticillium wilt resistance; Verticillium wilt resistance of the modern varieties (the average disease index was 43.75) was better than that of middle-stage varieties (48.60) and early-stage varieties (51.55); the resistance of the varieties from the Yellow River cotton-growing areas(the average disease index was 43.21) were better than those of other cotton-growing areas or geographic origins. The screening of disease-resistant varieties in this study provided excellent germplasm resources for genetic improvement of cotton Verticillium wilt resistance breeding.

Key words: Gossypium hirsutum L., core collection, Verticillium wilt, resistance identification

摘要: 棉花黄萎病是由大丽轮枝菌引起的土传维管束病害,对棉花生产造成了严重危害。核心种质代表着整个资源群体的遗传多样性,采用六棱塑料钵定量注射菌液法,在环境可控的人工生长室对419份陆地棉品种(系)组成的核心种质进行了黄萎病抗性鉴定与优异材料筛选。结果表明,419份陆地棉种质资源总体抗病材料偏少,共筛选出12份抗病材料,病情指数范围在21.67~25.00;耐病材料253份,病情指数分布在25.47~50.00,其他材料均表现为感病;缺乏对黄萎病免疫和高抗的材料;现代品种的平均病情指数为43.75,整体抗性都要好于中期、早期品种(平均病情指数分为48.60和51.55);来自我国黄河流域棉区的材料(平均病情指数为43.21)整体抗性要好于其他植棉区和地理来源品种的整体抗性。筛选出的抗病材料为棉花抗黄萎病遗传改良提供了优异资源。

关键词: 陆地棉, 核心种质, 黄萎病, 抗性鉴定

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