Effects of Excess Dietary Magnesium on Translucent Egg and Eggshell Microorganism of Xinyang Black Feather

doi:10.13304/j.nykjdb.2021.0546

Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (1): 197-205.DOI: 10.13304/j.nykjdb.2021.0546

• BIO-MANUFACTURING & RESOURCE AND ECOLOGY • Previous Articles

Effects of Excess Dietary Magnesium on Translucent Egg and Eggshell Microorganism of Xinyang Black Feather

Kui LU¹^,²^,³(), Fuwei LI⁴, Lihui ZHU³, Xiaoxiao HE¹^,²^,³, Lichun WEI¹^,²^,³, Yonghao ZHU¹^,²^,³, Huaxiang YAN³()

^1.National Demonstration Center for Experimental Teaching of Aquatic Science, Shanghai Ocean University, Shanghai 201120, China
^2.Research Center of Fish Nutrition and Environmental Ecology, Ministry of Agriculture and Ruzal Aflairs, Shanghai Ocean University, Shanghai 201120, China
^3.National Poultry Engineering Research Center, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
^4.Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China

Received:2021-07-04 Accepted:2021-11-22 Online:2023-01-15 Published:2023-04-17
Contact: Huaxiang YAN

饲粮中过量镁对新杨黑羽蛋鸡鸡蛋暗斑和蛋壳微生物组成的影响

卢奎¹^,²^,³(), 李福伟⁴, 朱丽慧³, 和笑笑¹^,²^,³, 卫利春¹^,²^,³, 朱永豪¹^,²^,³, 严华祥³()

^1.上海海洋大学，水产科学国家级实验教学示范中心，上海 201120
^2.上海海洋大学，农业农村部鱼类营养与环境生态研究中心，上海 201120
^3.上海市农业科学院畜牧兽医研究所，国家家禽工程技术研究中心，上海 201106
^4.山东省农业科学院家禽研究所，济南 250023

通讯作者: 严华祥
作者简介:卢奎 E-mail：1966361488@qq.com；
基金资助:
山东省重大科技创新工程项目(2019JZZY020611);国家现代农业产业技术体系建设项目(CARS-40);上海市农业科学院卓越团队项目(2018B)

Abstract

Abstract:

To investigate the effects of excessive dietary magnesium on the formation of translucent egg and eggshell microorganism composition of Xinyang black feather laying hens， 48 Xinyang black feather laying hens of 35-week-old whose eggs were prone to egg spotting， were randomly divided into three groups with 16 hens per group （one hen in one cage）. Hens were fed with experimental diets of 843.55 （A）， 2 009.80 （B） and 3 206.05 mg·kg^-1 （C）， respectively. The experiment lasted for 5 weeks. Translucent egg， egg quality and eggshell microorganism were detected. The results showed， compared with B and C treatments， the translucent egg grade of eggs in A treatment significantly decreased （P<0.01）， and the albumen height and Haugh unit significantly increased （P<0.05）. A total of 969 OTUs （operational taxonomic units） were detected by 16s rDNA sequencing， which were 670， 787 and 848 OTUs in A， B and C treatments， respectively. The OTUs increased with the increase of storage time. The OTU number of translucent egg grade 1 was significantly lower than those of other eggs with higher translucent egg grade （P<0.01）. The diets of lower magnesium concentration would reduce translucent egg rate and increase albumen height and Haugh unit.

Key words: Xinyang black feather, magnesium, translucent egg, microbes, eggshell

摘要：

为研究饲粮中镁对新杨黑羽蛋鸡鸡蛋暗斑形成和蛋壳微生物组成的影响，选取35周龄、所产鸡蛋易发暗斑的新杨黑羽蛋鸡48只，随机分成3组，每组16只（单笼饲养），分别饲喂镁浓度为843.55（A）、2 009.80（B）和3 206.05 （C） mg·kg^-1的试验饲粮，为期5周，检测鸡蛋暗斑、鸡蛋品质、蛋壳微生物的差异。结果表明，与B和C处理相比， A处理鸡蛋的暗斑等级极显著降低（P<0.01），蛋白高度和哈氏单位均显著提高（P<0.05）。通过16S rDNA测序，在蛋壳表面共检测到969个OTUs，其中，A、B、C处理分别检测到670、787、848个OTUs；随着储存时间的延长，微生物OTU类别数增加；1级暗斑等级的OTU类别数极显著低于暗斑等级较高的鸡蛋（P<0.01）。综上述所，饲粮中较低的镁浓度会减少鸡蛋暗斑，提高蛋白高度和哈氏单位。

关键词: 新杨黑羽蛋鸡, 镁, 鸡蛋暗斑, 微生物, 蛋壳

CLC Number:

S831.5

Kui LU, Fuwei LI, Lihui ZHU, Xiaoxiao HE, Lichun WEI, Yonghao ZHU, Huaxiang YAN. Effects of Excess Dietary Magnesium on Translucent Egg and Eggshell Microorganism of Xinyang Black Feather[J]. Journal of Agricultural Science and Technology, 2023, 25(1): 197-205.

卢奎, 李福伟, 朱丽慧, 和笑笑, 卫利春, 朱永豪, 严华祥. 饲粮中过量镁对新杨黑羽蛋鸡鸡蛋暗斑和蛋壳微生物组成的影响[J]. 中国农业科技导报, 2023, 25(1): 197-205.

Figures/Tables 11

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原料 Ingredient	A	B	C
玉米 Corn	60.00	60.00	60.00
豆粕 Soybean meal	24.50	24.50	24.50
麸皮 Bran	2.50	2.50	2.50
石粉 Limestone	0.00	3.75	7.50
贝壳粉 Shell powder	7.50	3.75	0.00
预混料 Premix	5.50	5.50	5.50
合计Total	100.00	100.00	100.00

原料 Ingredient	A	B	C
玉米 Corn	60.00	60.00	60.00
豆粕 Soybean meal	24.50	24.50	24.50
麸皮 Bran	2.50	2.50	2.50
石粉 Limestone	0.00	3.75	7.50
贝壳粉 Shell powder	7.50	3.75	0.00
预混料 Premix	5.50	5.50	5.50
合计Total	100.00	100.00	100.00

项目 Item	含量 Content
项目 Item	A	B	C
代谢能 Metabolic energy /（MJ·kg^-1）	10.7	10.7	10.7
粗蛋白质 Crude protein/%	17.8	17.8	17.8
粗纤维 Crude fiber/%	1.7	1.7	1.7
粗脂肪 Crude fat/%	2.3	2.3	2.3
总镁 Total Mg/g	0.08	0.20	0.32
总磷 Total P/%	0.4	0.4	0.4
赖氨酸 Lys/%	0.9	0.9	0.9
蛋氨酸 Met/%	0.3	0.3	0.3
总钙 Total Ca/%	3.7	3.7	3.7

项目 Item	含量 Content
项目 Item	A	B	C
代谢能 Metabolic energy /（MJ·kg^-1）	10.7	10.7	10.7
粗蛋白质 Crude protein/%	17.8	17.8	17.8
粗纤维 Crude fiber/%	1.7	1.7	1.7
粗脂肪 Crude fat/%	2.3	2.3	2.3
总镁 Total Mg/g	0.08	0.20	0.32
总磷 Total P/%	0.4	0.4	0.4
赖氨酸 Lys/%	0.9	0.9	0.9
蛋氨酸 Met/%	0.3	0.3	0.3
总钙 Total Ca/%	3.7	3.7	3.7

处理 Treatment	产蛋率 Laying rate/%	蛋重 Egg weight/g	蛋壳强度 Eggshell strength/（kg·cm^-2）	蛋黄颜色 Yolk color	蛋白高度 Albumen height/mm	哈氏单位 Haugh unit	蛋壳厚度 Eggshell thickness/mm
A	77.78±10.12	48.32±2.52	4.10±0.77	5.46±0.85	6.02±0.51 a	80.93±5.21 a	0.32±0.024
B	84.72±10.11	47.49±3.08	3.95±0.71	5.63±0.96	5.41±0.48 b	76.48±7.20 b	0.32±0.017
C	79.17±11.33	48.86±2.96	4.06±0.74	5.43±0.81	5.42±0.51 b	76.99±4.99 b	0.33±0.020
P值 P value	0.530	0.129	0.398	0.083	0.013	0.013	0.359

Effects of Excess Dietary Magnesium on Translucent Egg and Eggshell Microorganism of Xinyang Black Feather

饲粮中过量镁对新杨黑羽蛋鸡鸡蛋暗斑和蛋壳微生物组成的影响

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Figures/Tables 11

References 23

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