Effects of Different Light Qualities on Growth， Physiological Characteristics and Flavonoids Accumulation of Epimedium koreanum

doi:10.13304/j.nykjdb.2021.0676

Abstract

Abstract:

In order to study the effects of different light quality treatments on the growth， physiological characteristics and flavonoid accumulation of Epimedium koreanum， and determine the suitable light quality conditions for the cultivation and production of E. koreanum， 4 kinds of LED light source （white， red， yellow， blue） were set， and the white light treatment was as control （CK）. The growth indicators， physiological indicators and the content of flavonoids of E. koreanum under different light treatments， such as plant height， stem diameter， leaf area， leaf fresh weight， leaf dry weight， chlorophyll content， photosynthetic parameters， antioxidant enzyme activity， etc. were determined. The results showed that the leaf area， leaf fresh weight， leaf dry weight and chlorophyll content of E. koreanum significantly increased under blue light treatment. And the net photosynthetic under blue light treatment rate was higher than those under other light treatments. The blue light treatment promoted the accumulation of flavonoids in the leaves of E. koreanum. Compared with the white light treatment， the contents of total flavonoids， icariin and total flavonol glycosides under blue light treatment increased by 12.9%， 14.3% and 8.6%， respectively.Red and yellow light treatment were not conducive to the accumulation of flavonoids in the leaves of E. koreanum. Therefore， the leaf yield and flavonoid content of E. koreanum could be increased by covering the blue film or supplementing the LED blue light source at night during the cultivation of E. koreanum.

Key words: Epimedium koreanum, light quality, icariin, epimedonin, photosynthesis, LED light

摘要：

为了研究不同光质对朝鲜淫羊藿生长、生理和黄酮类成分积累的影响，确定适宜朝鲜淫羊藿栽培生产的光质条件，使用4种LED光源（白光、红光、黄光、蓝光）对盆栽朝鲜淫羊藿进行处理，以白光处理作为对照（CK），测定了各光质处理下朝鲜淫羊藿的株高、茎粗、叶面积、叶鲜重、叶干重等生长指标，叶绿素含量、光合作用参数、抗氧化酶活性等生理指标以及黄酮类成分含量。结果表明，蓝光处理下朝鲜淫羊藿的叶面积、叶鲜重、叶干重、叶绿素含量等显著增加，净光合速率高于其他光质处理；且促进了叶片黄酮类成分的积累，其中，总黄酮、淫羊藿苷和总黄酮醇苷含量较对照白光处理分别提高了12.9%、14.3%、8.6%。红光、黄光处理不利于朝鲜淫羊藿叶中黄酮类成分的积累。综上所述，在朝鲜淫羊藿栽培过程中可通过覆盖蓝色透光膜或在夜晚补充LED蓝色光源来提高叶产量和黄酮类成分含量。

关键词: 朝鲜淫羊藿, 光质, 淫羊藿苷, 朝藿定, 光合作用, LED光源

CLC Number:

S567.23+9

Ailin DAI, Yonggang ZHANG, Qiang AI, Xichao TENG, Limin YANG. Effects of Different Light Qualities on Growth， Physiological Characteristics and Flavonoids Accumulation of Epimedium koreanum[J]. Journal of Agricultural Science and Technology, 2022, 24(4): 85-92.

代艾林, 张永刚, 艾强, 滕熙超, 杨利民. 不同光质对朝鲜淫羊藿生长、生理和黄酮类成分积累的影响[J]. 中国农业科技导报, 2022, 24(4): 85-92.

Figures/Tables 5

References 24

1	应俊生.淫羊藿属(小檗科)花瓣的演化和地理分布格局的研究[J].植物分类学报,2002,40(6):481-489.
	YING J S. Petal evolution and distribution patterns of Epimedium L. (Berberidaceae) [J]. Acta Phytotaxonomica Sin., 2002, 40(6):481-489.
2	国家药典委员会.中华人民共和国药典 (一部)[M].北京:中国医药科技出版社,2020:340-342.
	Chinese Pharmacopoeia Commission.Pharmacopoeia of the People′s Republic of China (VolumeⅠ) [M]. Beijing: China Medical Science Press, 2020:340-342.
3	张永刚,韩梅,韩忠明,等.不同生境朝鲜淫羊藿生长与光合特征[J].生态学报,2012,32(5):1442-1449.
	ZHANG Y G, HAN M, HAN Z M, et al.. Growth and photosynthetic characteristics of Epimedium koreanum Nakai in different habitats [J]. Acta Ecol. Sin., 2012, 32(5):1442-1449.
4	张玉彬,刘文科,杨其长,等.LED红蓝光对西洋参植株生长、产量和皂苷含量的影响[J].中国农业科技导报,2020,22(4):171-176.
	ZHANG Y B, LIU W K, YANG Q C, et al.. Effects of LED red and blue light on growth,yield and saponin content of american ginseng (Panax quinquefolius) [J]. J. Agric. Sci. Technol., 2020, 22(4):171-176.
5	冷平生,苏淑钗,王天华,等.光强与光质对银杏作用及黄酮苷与萜类内脂含量的影响[J].植物资源与环境学报,2002,11(1):1-4.
	LENG P S, SU S C, WANG T H, et al.. Effects of light intensity and light quality on photosynthesis, flavonol glycoside and terpene lactone contents of Ginkgo biloba L.seedlings [J]. J. Plant Resour. Environ., 2002, 11(1):1-4.
6	YANG Q R, PAN J Q, SHEN G A, et al.. Yellow light promotes the growth and accumulation of bioactive flavonoids in Epimedium pseudowushanense [J]. J. Photochem. Photobiol. B, 2019, 197:111550-111556.
7	罗燕,张永刚,杨利民,等.光照强度对朝鲜淫羊藿生长、光合和叶绿素荧光参数特征的影响[J].东北林业大学学报,2018,46(7):44-48, 58.
	LUO Y, ZHANG Y G, YANG L M, et al.. Effect of light intensity on growth,photosynthetic and fluorescence characteristics of Epimedium koreanum Nakai [J]. J. Northeast For.Univ., 2018, 46(7):44-48, 58.
8	刘蓬蓬,王晶,雷天莉,等.不同生长环境朝鲜淫羊藿结实及有效成分差异分析[J].沈阳药科大学学报,2014,31(6):488-492.
	LIU P P, WANG J, LEI T L, et al.. Difference analysis on fruiting characteristics and active components in the different habitats of Epimedium koreanum Nakai [J]. J. Shenyang Pharmaceutical Univ., 2014, 31(6):488-492.
9	高俊凤. 植物生理学实验指导[M].北京: 高等教育出版社,2006:1-287.
10	梁宗锁,李倩,徐文晖.不同光质对丹参生长及有效成分积累和相关酶活性的影响[J].中国中药杂志,2012,37(12):2055-2060.
	LIANG Z S, LI Q, XU W H. Effects of different light quality on growth, active ingredients and enzymes activities of Salvia miltiorrhiza [J]. China J. Chin. Mat. Med., 2012, 37(12):2055-2060.
11	彭亮,赵停,杨冰月,等.不同光质光强对远志生长和相关酶活性及成分的影响[J].中草药,2018,49(21):5005-5009.
	PENG L, ZHAO T, YANG B Y, et al.. Effects of light quality and intensity on growth, related enzymes activity, and components of Polygala tenuifolia [J]. Chin. Trad. Herbal Drugs, 2018, 49(21):5005-5009.
12	谢德金,李静文,叶友杰,等.光质对草珊瑚幼苗生长及其生理生化基础的影响[J].草业学报,2020,29(8):104-115.
	XIE D J, LI J W, YE Y J, et al.. Effects of light quality on growth,and physiological and biochemical traits of Sarcandra glabra seedlings [J]. Acta Pratac. Sin., 2020, 29(8):104-115.
13	李小明,潘俊清,落艳娇,等.不同光强下光质对拟巫山淫羊藿生长发育和淫羊藿苷类黄酮含量的影响[J].中国中药杂志,2020,45(11):2502-2508.
	LI X M, PAN J Q, LUO Y J, et al.. Effects of light quality on growth and icariin flavonoid content of Epimedium pseudowushanense under different light intensity [J]. China J. Chin. Mat. Med., 2020, 45(11):2502-2508.
14	JOHKAN M, SHOJI K, GOTO F, et al.. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce [J]. Hort. Sci., 2010, 45:1809-1814.
15	张松勇.不同光质对紫苏光合特性的影响[J].热带作物学报,2017,38(3):444-449.
	ZHANG S Y. Effects of different led light qualities on photosynthetic characteristics of Basil [J]. Chin. J. Tropical Crops, 2017, 38(3):444-449.
16	程小燕,杨志玲,杨旭,等.不同光质对三叶崖爬藤生长及有效成分含量的影响[J].林业科学研究,2018,31(5):98-103.
	CHENG X Y, YANG Z L, YANG X, et al.. Effects of light quality on the growth and content of active ingredients of Tetrastigma hemsleyanum [J]. For. Res., 2018, 31(5):98-103.
17	卢素萍,赵铭钦,刘鹏飞,等.光质对烤烟叶片衰老过程中内源激素含量和膜脂过氧化的影响[J].中国农业科技导报,2016,18(6):44-51.
	LU S P, ZHAO M Q, LIU P F, et al.. Effect of light quality on endogenous hormone content and lipid peroxidation of membrane system during aging process of flue-cured tobacco leaf [J]. J. Agric. Sci. Technol., 2016, 18(6):44-51.
18	张鹏飞,刘亚令,梁建萍,等.不同光质处理对蒙古黄芪幼苗根系生物量及有效成分积累的影响[J].草地学报,2015,23(4):838-843.
	ZHANG P F, LIU Y L, LIANG J P, et al.. Effects of different light quality on growth and accumulation of active ingredient in Astragalus membranaceus [J]. Acta Pratac. Sin., 2015, 23(4):838-843.
19	赵德修,李茂寅,邢建民,等.光质、光强和光期对水母雪莲愈伤组织生长和黄酮生物合成的影响[J].植物生理学报,1999,25(2):127-132.
	ZHAO D X, LI M Y, XING J M, et al.. Effects of light on cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim [J]. Acta Phytophysiol. Sin., 1999, 25(2):127-132.
20	GUO B, LIU Y G, YAN Q, et al.. Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim [J]. Plant Growth Regul., 2007, 52:259-263.
21	THWE A A, KIM Y B, LI X, et al.. Effects of light-emitting diodes on expression of phenylpropanoid biosynthetic genes and accumulation of phenylpropanoids in Fagopyrum tataricum sprouts [J]. J. Agric. Food Chem., 2014, 62(21):4839-4845.
22	潘俊倩,佟曦然,郭宝林,等.光对植物黄酮类化合物的影响研究进展[J].中国中药杂志,2016,41(20):3897-3903.
	PAN J Q, TONG X R, GUO B L, et al.. Progress of effects of light on plant flavonoids [J]. China J. Chin. Mat. Med., 2016, 41(20):3897-3903.
23	肖开前,赖荣才,林仁穗,等.不同培养方式对金线莲主要化学成分的影响[J].中药材,2014,37(4):553-556.
	XIAO K Q, LAI R C, LIN R S, et al.. Effects of different culture conditions on main chemical compositions of Anoectochilus roxburghii [J]. J. Chin. Med. Mat., 2014, 37(4):553-556.
24	刘洋,秦健,周明明,等.光质和基因型对青钱柳叶黄酮类化合物积累的影响[J].南京林业大学学报(自然科学版),2018,42(3):99-104.
	LIU Y, QIN J, ZHOU M M, et al.. Influence of light quality and genetype on flavonoid accumulation in the leaves of Cyclocarya paliurus [J]. J. Nanjing For. Univ. (Nat. Sci.), 2018, 42(3):99-104.

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter/mm	叶面积 Leaf area/cm²	叶鲜重 Leaf fresh weight/g	叶干重 Leaf dry weight/g
白光White light	9.63±0.15 a	4.83±0.31 a	73.03±2.57 ab	5.45±0.29 b	2.08±0.10 c
红光Red light	10.17±0.35 a	4.43±0.40 a	70.03±2.72 b	5.94±0.11 a	2.19±0.15 bc
黄光Yellow light	10.43±0.50 a	4.30±0.40 a	75.75±3.72 a	6.26±0.16 a	2.62±0.08 a
蓝光Blue light	10.10±0.60 a	4.17±0.38 a	77.60±1.73 a	6.17±0.34 a	2.37±0.07 b

处理 Treatment	株高 Plant height/cm	茎粗 Stem diameter/mm	叶面积 Leaf area/cm²	叶鲜重 Leaf fresh weight/g	叶干重 Leaf dry weight/g
白光White light	9.63±0.15 a	4.83±0.31 a	73.03±2.57 ab	5.45±0.29 b	2.08±0.10 c
红光Red light	10.17±0.35 a	4.43±0.40 a	70.03±2.72 b	5.94±0.11 a	2.19±0.15 bc
黄光Yellow light	10.43±0.50 a	4.30±0.40 a	75.75±3.72 a	6.26±0.16 a	2.62±0.08 a
蓝光Blue light	10.10±0.60 a	4.17±0.38 a	77.60±1.73 a	6.17±0.34 a	2.37±0.07 b

处理 Treatment	总黄酮 Total flavone/%	朝藿定A Epimedin A/%	朝藿定B Epimedin B/%	朝藿定C Epimedin C/%	淫羊藿苷 Icariin/%	总黄酮醇苷 Total flavonol glycosides/%
白光White light	18.223±0.096 b	0.607±0.035 b	0.659±0.025 c	0.678±0.022 a	0.767±0.045 b	2.711±0.137 b
红光Red light	14.793±0.358 d	0.447±0.025 c	0.807±0.028 a	0.453±0.035 b	0.583±0.030 c	2.290±0.204 c
黄光Yellow light	15.330±0.386 c	0.440±0.036 c	0.787±0.035 b	0.387±0.039 c	0.643±0.025 c	2.257±0.065 c
蓝光Blue light	20.577±0.162 a	0.727±0.021 a	0.657±0.031 c	0.683±0.031 a	0.877±0.045 a	2.944±0.052 a

处理 Treatment	总黄酮 Total flavone/%	朝藿定A Epimedin A/%	朝藿定B Epimedin B/%	朝藿定C Epimedin C/%	淫羊藿苷 Icariin/%	总黄酮醇苷 Total flavonol glycosides/%
白光White light	18.223±0.096 b	0.607±0.035 b	0.659±0.025 c	0.678±0.022 a	0.767±0.045 b	2.711±0.137 b
红光Red light	14.793±0.358 d	0.447±0.025 c	0.807±0.028 a	0.453±0.035 b	0.583±0.030 c	2.290±0.204 c
黄光Yellow light	15.330±0.386 c	0.440±0.036 c	0.787±0.035 b	0.387±0.039 c	0.643±0.025 c	2.257±0.065 c
蓝光Blue light	20.577±0.162 a	0.727±0.021 a	0.657±0.031 c	0.683±0.031 a	0.877±0.045 a	2.944±0.052 a

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[5]	LIU Shidou1, ZHU Xinping1,2*, ZHAO Yi1, WANG Boyan1, HAN Yaoguang1, YANG Beibei1, LU Zhi1, JIA Hongtao1,2. Cotton Stalk Biochar Retarding Stress of Cadmium on Growth of Rice [J]. Journal of Agricultural Science and Technology, 2020, 22(4): 139-146.
[6]	LI Meng, CHEN Dong, LI Xiuni, LI Linlin, WANG Ronghao, SHI Xiangdong*. Influenes of Exogenous Melatonin on Antioxidant and Photosynthetic Characteristics of Tobacco Seedlings Under Salt Stress [J]. Journal of Agricultural Science and Technology, 2019, 21(2): 141-147.
[7]	WANG Jian, SHI Jing. Prediction Model of SVR Photosynthetic Rate Based on Chemotaxis-Improved Particle Swarm Optimization [J]. Journal of Agricultural Science and Technology, 2018, 20(12): 74-82.
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[9]	LU Su-ping1, ZHAO Ming-qin1*, LIU Peng-fei1, LI Yu-e2, Fu-bo1. Effect of Light Quality on Endogenous Hormone Content and Lipid Peroxidation of Membrane System during Aging Process of Flue-cured Tobacco Leaf [J]. Journal of Agricultural Science and Technology, 2016, 18(6): 44-51.
[10]	HE Wei, YANG Zhen-chao*, CAI Hua, WANG Da-fei, WANG Xiao-xu. Effects of Light Quality on Growth and Morphologenesis Regulation of Vegetables: A Review [J]. Journal of Agricultural Science and Technology, 2016, 18(2): 9-18.
[11]	SHEN Ya-jun1, CUI Xue-an2, ZHANG Zhi-guo2, WU Jin-xia2*. Gene Mapping of a Rolled Leaf Mutant rlm1 in Rice [J]. Journal of Agricultural Science and Technology, 2016, 18(2): 25-30.
[12]	DUAN Long\\|fei1, SHANG Ai\\|qin1*, YANG Min\\|sheng2, WANG Jin\\|mao2, ZUO Li\\|hui2. Studies on Diurnal Variations of Photosynthetic Characteristics and Chlorophyll Fluorescence Parameters of Different Clones of Ulmus pumila cv. ‘Zhonghua jinye’ [J]. , 2014, 16(6): 21-27.
[13]	CAI Hong-chang, CUI Hai-xin, CUI Jin-hui. Effects of Different Light Qualities on the Quality of \|Tomato Plug Seedlings [J]. , 2010, 12(3): 114-118.