Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (1): 119-127.DOI: 10.13304/j.nykjdb.2021.0888
• ANIMAL AND PLANT HEALTH • Previous Articles
Yudan ZHANG1(), Weimin WANG2, Bo NI3, Xiaohan MA1, Junling LI1, Zicheng XU1, Wei JIA1(
), Jiuchang SHI2(
)
Received:
2021-10-18
Accepted:
2022-04-12
Online:
2023-01-15
Published:
2023-04-17
Contact:
Wei JIA,Jiuchang SHI
张豫丹1(), 王卫民2, 倪博3, 马晓寒1, 李俊领1, 许自成1, 贾玮1(
), 史久长2(
)
通讯作者:
贾玮,史久长
作者简介:
张豫丹E-mail:519086134@qq.com
基金资助:
CLC Number:
Yudan ZHANG, Weimin WANG, Bo NI, Xiaohan MA, Junling LI, Zicheng XU, Wei JIA, Jiuchang SHI. Extraction Optimization of Chlorogenic Acid from Tobacco Stalk and Its Antifungal Effect[J]. Journal of Agricultural Science and Technology, 2023, 25(1): 119-127.
张豫丹, 王卫民, 倪博, 马晓寒, 李俊领, 许自成, 贾玮, 史久长. 烟草秸秆绿原酸提取工艺优化及其抑菌效果研究[J]. 中国农业科技导报, 2023, 25(1): 119-127.
水平 Level | 因素 Factor | ||
---|---|---|---|
A:料液比 Solid-liquid ratio/(g·mL-1) | B:乙醇体积分数 Ethanol volume fraction/% | C:提取温度 Extraction teperature/℃ | |
-1 | 1∶15 | 50 | 30 |
0 | 1∶20 | 60 | 40 |
1 | 1∶25 | 70 | 50 |
Table 1 Factors and levels of response surface design
水平 Level | 因素 Factor | ||
---|---|---|---|
A:料液比 Solid-liquid ratio/(g·mL-1) | B:乙醇体积分数 Ethanol volume fraction/% | C:提取温度 Extraction teperature/℃ | |
-1 | 1∶15 | 50 | 30 |
0 | 1∶20 | 60 | 40 |
1 | 1∶25 | 70 | 50 |
编号 No. | 因素 Factor | 绿原酸含量 CGA content/(mg·g-1) | ||
---|---|---|---|---|
A | B | C | ||
1 | -1 | -1 | 0 | 1.51 |
2 | 1 | -1 | 0 | 2.54 |
3 | -1 | 1 | 0 | 1.46 |
4 | 1 | 1 | 0 | 2.49 |
5 | -1 | 0 | -1 | 1.45 |
6 | 1 | 0 | -1 | 2.58 |
7 | -1 | 0 | 1 | 1.58 |
8 | 1 | 0 | 1 | 2.65 |
9 | 0 | -1 | -1 | 2.21 |
10 | 0 | 1 | -1 | 2.18 |
11 | 0 | -1 | 1 | 2.33 |
12 | 0 | 1 | 1 | 2.27 |
13 | 0 | 0 | 0 | 2.08 |
14 | 0 | 0 | 0 | 2.11 |
15 | 0 | 0 | 0 | 2.14 |
16 | 0 | 0 | 0 | 2.06 |
17 | 0 | 0 | 0 | 2.16 |
Table 2 Experimental results of response surface design
编号 No. | 因素 Factor | 绿原酸含量 CGA content/(mg·g-1) | ||
---|---|---|---|---|
A | B | C | ||
1 | -1 | -1 | 0 | 1.51 |
2 | 1 | -1 | 0 | 2.54 |
3 | -1 | 1 | 0 | 1.46 |
4 | 1 | 1 | 0 | 2.49 |
5 | -1 | 0 | -1 | 1.45 |
6 | 1 | 0 | -1 | 2.58 |
7 | -1 | 0 | 1 | 1.58 |
8 | 1 | 0 | 1 | 2.65 |
9 | 0 | -1 | -1 | 2.21 |
10 | 0 | 1 | -1 | 2.18 |
11 | 0 | -1 | 1 | 2.33 |
12 | 0 | 1 | 1 | 2.27 |
13 | 0 | 0 | 0 | 2.08 |
14 | 0 | 0 | 0 | 2.11 |
15 | 0 | 0 | 0 | 2.14 |
16 | 0 | 0 | 0 | 2.06 |
17 | 0 | 0 | 0 | 2.16 |
来源 Source | 平方和 Sun of squares | 自由度 DF | 均方 Mean freedom | F值 F value | P值 P value | 显著性 Statistical significance |
---|---|---|---|---|---|---|
模型 Model | 2.42 | 9 | 0.27 | 215.52 | <0.000 1 | *** |
A | 2.26 | 1 | 2.26 | 1812.80 | <0.000 1 | *** |
B | 0.004 5 | 1 | 0.004 5 | 3.58 | 0.100 3 | |
C | 0.02 | 1 | 0.02 | 16.36 | 0.004 9 | ** |
AB | 0.004 6 | 1 | 0.004 6 | 0.037 | 0.853 8 | |
AC | 0.001 2 | 1 | 0.001 2 | 0.94 | 0.364 1 | |
BC | 0.001 7 | 1 | 0.001 6 | 0.14 | 0.724 0 | |
A2 | 0.092 | 1 | 0.092 | 73.53 | <0.000 1 | *** |
B2 | 0.005 | 1 | 0.005 | 4.00 | 0.085 6 | |
C2 | 0.042 | 1 | 0.042 | 33.84 | 0.000 7 | ** |
残差 Residuel | 0.008 8 | 7 | 0.001 2 | — | — | |
失拟项 Lack of fit | 0.002 2 | 3 | 0.007 2 | 0.44 | 0.736 2 | |
纯误差 Pure error | 0.006 6 | 4 | 0.001 6 | — | — | |
总差 Cor total | 2.43 | 16 | — | — | — |
Table 3 Variance analysis results of regression model
来源 Source | 平方和 Sun of squares | 自由度 DF | 均方 Mean freedom | F值 F value | P值 P value | 显著性 Statistical significance |
---|---|---|---|---|---|---|
模型 Model | 2.42 | 9 | 0.27 | 215.52 | <0.000 1 | *** |
A | 2.26 | 1 | 2.26 | 1812.80 | <0.000 1 | *** |
B | 0.004 5 | 1 | 0.004 5 | 3.58 | 0.100 3 | |
C | 0.02 | 1 | 0.02 | 16.36 | 0.004 9 | ** |
AB | 0.004 6 | 1 | 0.004 6 | 0.037 | 0.853 8 | |
AC | 0.001 2 | 1 | 0.001 2 | 0.94 | 0.364 1 | |
BC | 0.001 7 | 1 | 0.001 6 | 0.14 | 0.724 0 | |
A2 | 0.092 | 1 | 0.092 | 73.53 | <0.000 1 | *** |
B2 | 0.005 | 1 | 0.005 | 4.00 | 0.085 6 | |
C2 | 0.042 | 1 | 0.042 | 33.84 | 0.000 7 | ** |
残差 Residuel | 0.008 8 | 7 | 0.001 2 | — | — | |
失拟项 Lack of fit | 0.002 2 | 3 | 0.007 2 | 0.44 | 0.736 2 | |
纯误差 Pure error | 0.006 6 | 4 | 0.001 6 | — | — | |
总差 Cor total | 2.43 | 16 | — | — | — |
编号 No. | 料液比 Material ratio/(g·mL-1) | 提取温度 Extraction temperature/℃ | 乙醇体积分数 Ethanol volume fraction/% | 绿原酸含量 Chlorogenic acid content/(mg·g-1) |
---|---|---|---|---|
1 | 1∶15 | 50 | 50 | 3.51 |
2 | 1∶15 | 50 | 50 | 3.47 |
3 | 1∶15 | 50 | 50 | 3.50 |
Table 4 Extraction yield of chlorogenic acid in validation experiments
编号 No. | 料液比 Material ratio/(g·mL-1) | 提取温度 Extraction temperature/℃ | 乙醇体积分数 Ethanol volume fraction/% | 绿原酸含量 Chlorogenic acid content/(mg·g-1) |
---|---|---|---|---|
1 | 1∶15 | 50 | 50 | 3.51 |
2 | 1∶15 | 50 | 50 | 3.47 |
3 | 1∶15 | 50 | 50 | 3.50 |
Fig. 7 Effect of chlorogenic acid on the diameter of P. nicotianaeNote:Different small letters in the figure indicate significant difference at P<0.05 level.
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