Abstract:

COI1 （COR-insensitive 1） and Skp1-Cullin-F-box protein constitute the SCF^COI1 complex，a core component of jasmine signal transduction， which plays an important role in plant growth development and response to stress. A total of 48 COI genes were identified in Gossypium hirsutum L.， which were unevenly distributed in 21 chromosomes and mainly located in the nucleus and cytoplasm. According to the phylogenetic relationship， the GhCOI genes were divided into 3 subgroups （Ⅰ~Ⅲ）. Collinearity analysis showed that COI genes expanded by segmental duplication in upland cotton. The GhCOI genes contained 1 ~ 9 exons， and there were 6 stress response elements in their promoter regions. The transcriptome data showed that 66.7%， 56.25%， 68.8% and 27.1% of genes were highly expressed under NaCl， PEG， heat and cold stress， respectively， and some genes responded to multiple abiotic stresses simultaneously. The qRT-PCR results showed that GhCOI5-A06 was a candidate gene with strong response to NaCl and PEG stress， which showed that GhCOIs involved in response to abiotic stress. Above results laid foundation for further analysis of this family genes function in cotton.

Key words: Gossypium hirsutum L., COI family, gene indentification, abiotic stress

摘要：

COI1（COR-insensitive 1）与Skp1-Cullin-F-box蛋白组成茉莉素信号转导的核心组分SCF^COI1复合物，在植物生长发育和响应逆境胁迫中发挥重要作用。从全基因组水平上鉴定出48个陆地棉COI家族基因，其不均匀地分布在21条染色体上，主要定位于细胞核和细胞质中。根据系统发育关系将其分为3个亚族（Ⅰ～Ⅲ），共线性分析发现COI基因以片段复制方式在陆地棉中进行扩张。陆地棉COI基因含有1～9个外显子，其启动子区域共存在6种逆境胁迫响应元件。转录组数据显示，分别有66.7%、56.25%、68.8%和27.1%的基因在NaCl、PEG、热和冷胁迫下表达量升高，且部分基因同时响应多种非生物胁迫；qRT-PCR验证结果显示，GhCOI5-A06强烈响应NaCl和PEG胁迫，是潜在的候选基因，表明GhCOI参与非生物胁迫应答。上述结果为后续深入解析该家族基因的功能奠定基础。

关键词: 陆地棉, COI家族, 基因鉴定, 非生物胁迫