Abstract:

A multiplex PCR system was designed and optimized with 35S and Nos primer and other 6 special primers which are designed according to inserted genes, plasmid constructions and inserted sites to detect 6 transgenic maize events which are permitted to import as product material by China, A primarily designed DNA chip coupled with the multiplex PCR system was also used to detect mixed GM maize samples, We attempted to construct a rapid GM maize events detection and confirmation method.

Key words: GM maize , multiplex PCR , DNA chips

摘要：

以我国批准进口用作加工原料的6种转基因玉米为材料,根据其外源基因、载体构建及插入位点,应用6对特异性引物及35S、NOS引物,设计并优化了复合PCR反应体系,对其进行转基因成分检测.同时尝试利用复合PCR与DNA芯片相结合,对不同转基因玉米混合样品进行检测,探索建立转基因事件快速检测和确认方法.

关键词: 转基因玉米 复合PCR DNA芯片