Abstract:

A 2.4 kb DNA fragment encoding maltooligosyl trehalose synthase was cloned from

Corynebacterium glutamicum by PCR. It was inserted into prokaryotic expression vector

pET-30a and then was introduced into the host Escherichia coli BL21 (DE3) pLysS. A high

yield of the recombinant enzyme at a molecular weight of 90 kDa was obtained by IPTG

induction. The soluble recombinant enzyme accounted for 45.3% of the total cell proteins

in supernatant. This recombinant enzyme was capable of decreasing the DE value of dextrin

and producing a maltooligosyl trehalose mixture.

Key words: maltoologosyl trehalose synthase, malt dextrin, Corynebacterium glutamicum, recombinant expression

摘要：

采用PCR方法从谷氨酸棒杆菌（Corynebacterium glutamicum）基因组中分离得到2.4 kb的麦芽寡糖基

海藻糖合成酶基因（TreY）,将其插入原核表达载体pET-30a中,转化大肠杆菌BL21（DE3）pLysS获得重

组基因工程菌。经IPTG诱导表达得到约90 kDa的目的蛋白,可溶性蛋白占细胞总蛋白的45.3%。重组酶

作用于麦芽糊精,可使其DE值降低,得到麦芽寡糖基海藻糖的混合物。

关键词: 麦芽寡糖基海藻糖合成酶；麦芽糊精；谷氨酸棒杆菌；重组表达