Abstract:

Two different types of L. hedysaroides with yellow-green leaf or dark-green leaf showed

obvious differences in micromorphological features of pod-coat and seed-coat by scanning

electron microscope (SEM) observation. These micromorphological features can be used for

identification of two different types of L. hedysaroides. ISSR-PCR molecular marker was

also used to identify two different types of L. hedysaroides. Among eight primers for

experiments, the primer UBC-880 was able to distinguish the examined two different types

of L. hedysaroides. L. hedysaroides with dark-green leaf has a polymorphic band in

500~600 bp. ISSR-PCR molecular marker can be used to identify two different types of L.

hedysaroides on DNA level.

Key words: Lespedeza hedysaroides；SEM；ISSR-PCR；micromorphology；molecular marker

摘要：

通过扫描电镜观察到叶片浓绿型和叶片黄绿型2个不同类型尖叶胡枝子的豆荚和种皮微形态具有明显差

异,其特征可作为2个不同类型尖叶胡枝子的鉴定依据。还采用ISSR-PCR分子标记方法对2个不同类型尖

叶胡枝子进行了鉴定。实验共考察8个引物,筛选出引物UBC-880可以独立区分开2个不同类型的尖叶胡

枝子,叶片浓绿型尖叶胡枝子可产生1条500~600 bp大小的多态性谱带,表明ISSR-PCR方法可为鉴定2个

不同类型尖叶胡枝子提供DNA分子水平上的依据。

关键词: 尖叶胡枝子；扫描电镜；ISSR-PCR；微形态；分子标记