Interaction between APX and ATP6 by the Yeast Two-Hybrid System in Brassica napus L.

›› 2009, Vol. 11 ›› Issue (4): 95-99.

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Interaction between APX and ATP6 by the Yeast Two-Hybrid System in Brassica napus L.

LI Zhi ^1,2, LIU Zhi-bin ¹, XIANG Jun-bei ¹, YUE Yu-fei ¹, WANG Yan¹, Wan Jian-mei¹, YANG Yi ¹, LI Xu-feng ¹

1.Key Laboratory of Bio-Resources and Eco-environment of Ministry of Education, College of Life Science,
Sichuan University, Chengdu 610064;2.Horticulture Institute of Sichuan,Academy of Agriculture Sciences, Chengdu 610066, China

Received:2009-03-18 Revised:2009-04-14 Online:2009-08-15 Published:2009-07-24

利用酵母双杂交系统研究甘蓝型油菜APX和ATP之间的相互作用

李志^1,2,刘志斌¹,向俊蓓¹,岳渝飞¹,王艳¹,王健美¹,杨毅¹,李旭峰¹

1.四川大学生命科学学院生物资源与生态环境教育部重点实验室, 成都 610064;2.四川省农业科学院园艺研究所, 成都 610066

通讯作者: 李旭峰，教授,博士生导师,主要从事植物分子遗传学研究。Tel:028-85410957;E-mail:yangyi528@vip.sina.com
作者简介:李志,硕士研究生,研究方向为植物分子遗传。
基金资助:
国家自然科学基金项目（30570986）资助。

Abstract

Abstract:

The ascorbate peroxidase (APX) was the key enzyme eliminating H₂O₂ in stress condition of ASA-GSH cycle in plant. In order to explore the mechanism of APX in cytoplasmic male sterility (CMS), we constructed different length APX cDNA sequence fusion vector by pGADT7 plasmid, analyzed the interacting area and point between ATP6 and APX by the yeast two-hybrid system in Brassica napus. The results showed that the interacting area with ATP6 amino acid sequence pointed at 250~439 aa of C-end in APX amino acid sequence.

Key words: yeast two-hybrid system, Brasscia napus L., ATP6, APX（ascorbate peroxidase）

摘要：

抗坏血酸过氧化物酶(APX)是植物中抗坏血酸-谷胱甘肽(ASA-GSH)循环中清除逆境条件下产生的H₂O₂的关键酶之一。为探索APX在细胞质雄性不育（CMS）中的作用机理，本实验通过构建含不同长度的APX cDNA序列的pGADT7融合载体，利用酵母双杂交系统分析ATP6和APX之间的相互作用及其作用位点，结果显示APX的C端250~439位氨基酸序列与ATP6的氨基酸序列具有相互作用。

关键词: 酵母双杂交；甘蓝型油菜（Brasscia napus L.）；ATP6；抗坏血酸过氧化物酶

CLC Number:

LI Zhi,2, LIU Zhi-bin, XIANG Jun-bei, et al.. Interaction between APX and ATP6 by the Yeast Two-Hybrid System in Brassica napus L.[J]. , 2009, 11(4): 95-99.

李志,刘志斌,向俊蓓,岳渝飞,王艳,王健美,杨毅,李旭峰. 利用酵母双杂交系统研究甘蓝型油菜APX和ATP之间的相互作用[J]. , 2009, 11(4): 95-99.

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Interaction between APX and ATP6 by the Yeast Two-Hybrid System in Brassica napus L.

利用酵母双杂交系统研究甘蓝型油菜APX和ATP之间的相互作用

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