Abstract:

p23 protein, found in Hsp90 chaperone complexes, was ubiquitous and highly conserved in eukaryotes. The ortholog in silkworm was named as p23-like protein. The results of RT-PCR showed that Bm-p23-like gene expressed in 8 tissues, including ovary, testis and so on, in the 5th-instar day-6 larvae. However there was big difference of copy number in different tissues which might be resulted from the vigor of different tissues. Using real-time PCR, we monitored the expression amount of Bm-p23-like and Bm-hsp90 under temperature stress. The results showed that the expressions of both Bm-p23-like and Bm-hsp90 increased and the ratio of hsp90/p23 was 42.23, while the non-stress control was 25.10 under high temperature. Under low temperature, the copy number of Bm-p23-like gene was 19.90 folds of the control, while that of Bm-hsp90 was only 0.60 folds of the control. Consequently, the ratio of the two genes was 0.89. The results above enabled us to deduce that Bm-p23-like protein possessed its own functions under emperature stress.

Key words: Bombyx mori, p23-like protein, expression analysis, real-time PCR

摘要：

p23蛋白发现于Hsp90分子伴侣复合体中,在真核生物中广泛存在且高度保守。家蚕中的同源蛋白命名为类p23蛋白。逆转录PCR显示类p23蛋白基因Bm-p23-like在家蚕5龄第6 d幼虫的卵巢、睾丸等8个组织中有表达,但拷贝数有较大差异,可能与组织的活跃程度有关。利用实时定量PCR技术对Bm-p23-like及hsp90在家蚕温度胁迫条件下的表达进行定量分析,结果显示：在高温条件下,该基因与hsp90表达较对照组均有增加,二者比值是42.23（非胁迫对照组是25.10）；在低温条件下,该基因的拷贝数是对照的19.90倍,而hsp90是对照的0.60倍,二者比值仅为0.89。因此,我们推测在温度胁迫条件下家蚕类p23蛋白具有独立于Hsp90的功能。

关键词: 家蚕;类p23蛋白;表达分析;实时荧光定量PCR