Abstract:

Gene targeting is one of the important tools in reversed genetics and a powerful tool in functional characterization of plant genes. Positive-negative selection system is used to identify correct transformants upon gene targeting. Using selection systems can reduce work load and greatly improve gene targeting efficiency. In recent years, with continuous studies and improvement, the use of positive-negative selection in plant gene targeting has become normal. This paper expounds the principle of the method and summarized its research progress, which will be of significant value f or gene targeting study in the future.

Key words: gene targeting, positive-negative selection, codA, DT-A

摘要：

基因打靶是反向遗传学研究的重要工具,在植物基因功能研究等方面具有重要意义。正负筛选系统是一种筛选发生正确基因打靶转化株的方法,能够减少筛选的工作量,使植物基因打靶效率大大提高。通过近些年的研究改进,正负筛选在植物基因打靶研究中的应用逐渐成熟,就植物基因打靶研究中正负筛选系统的原理及研究进展进行综述,对今后的基因打靶研究具有重要的参考价值。

关键词: 基因打靶;正负筛选;codA;DT-A