Abstract:

Quantification of deoxyribonucleic acid are key applications encompassing food safety testing, medical diagnostics, detection of GMOs and pathogen identification. Its broad applications have encouraged a rapid and sustained development of the technology, especially the primary methods, resulted in reliability, comparability, and consistency. The analysis of deoxyribonucleic acid and the testing capability are crucially dependent on the measurements by primary methods. In this review, we discuss the advances in development of primary methods, including the advantages and disadvantages. The prospects for the development and applications of primary methods are also discussed.

Key words: quantification of deoxyribonucleic acid, primary methods, IDMS, ICPMS/OES, dPCR

摘要：

脱氧核糖核酸定量分析是食品安全检验、医疗诊断、转基因检测与病原微生物鉴定等相关领域的基础。常见的定量方法种类繁多,如紫外分光光度法、荧光染料法和实时荧光定量PCR法等等,各有所长,但测量结果缺乏可靠性、可比性和一致性。随着研究的深入与应用要求的提高,基准方法成为了核酸分析以及核酸测量能力评估的关键。本文综述了近年来三种成为潜在脱氧核糖核酸定量基准方法的研究发展,评述了各种方法的技术优势、不足与应用潜力,以期为其相关研究和利用提供参考。

关键词: 脱氧核糖核酸定量;基准方法;IDMS;ICPOES/MS;dPCR