Abstract:

Detection and evaluation of the unintended effects for genetically modified (GM) plants is one of the most important contents of safety assessment of GM plants. The definition, causes and progresses on the technologies for detecting and evaluating the unintended effects of GM plants were introduced. And the technical difficulties and existing problems about detection and evaluation of GM plants unintended effects were also pointed out. The potential application about the DNA microarray analysis technology used in detecting and evaluating the unintended effects of GM plants was specially emphasized. Besides, we had found out a non-uniform distribution pattern for differentially expressed genes of GM plants at different developmental stages and environments. This safety assessment mode may provide an experimental and theoretical basis for establishment of efficient, comprehensive, objective, impartial technologies, which can be applied to detecting and evaluating the unintended effects of GM plants.

Key words: genetically modified plants, unintended effects, DNA microarray analysis, safety assessment

摘要：

检测和评价转基因植物非预期效应是转基因植物安全评价不可或缺的重要内容之一。介绍了转基因植物非预期效应的定义、成因及其检测评价技术的发展，指出了转基因植物非预期效应检测评价技术的难点和存在的问题。阐述了基因芯片分析技术在检测和评价转基因植物非预期效应中的应用前景，特别介绍了转基因植物在不同发育阶段和生长条件下差异表达基因的分布模型，并提出了利用基因芯片分析技术，从差异基因到代谢途径，再到非预期效应的转基因植物安全评价模式。这一评价模式可为今后创建高效、全面、客观、公正的转基因植物非预期效应检测和评价技术提供可能的实验模型和理论依据。

关键词: 转基因植物；非预期效应；基因芯片；安全评价