Abstract:

Microalgae has obvious effect on water purification, this paper explored phosphorus removal capacity of Chlorella pyrenoidosa in open system and purification effect of wastewater by Algae\|Daphnia system. It was indicated that after incubated in sterile wastewater for 8 h, algae can achieve max amount of phosphorus absorption, (2.99±045)×10-10 mg/cell. After incubated in sterile wastewater for 96 h, the rate of phosphorus removal reached to 75%, when inoculation quantity of algae was between 1.00×105 cells/mL and 3.72×106 cells/mL. The phosphorus removal capacity of each multiplied algae cell（saturated phosphorus content） was （1.46±0.05）×10-10 mg/cell in the sterile wastewater, and it was（1.50±0.50）×10-10 mg/cell in the open system from 72 h to 216 h, under the condition that nutrition was added to the system every day. The amount of phosphorus removal was （0.06±0.01）mg/（L·d）, when the system worked well and algaes density was between 1.11×106 and 3.79×106 cells/mL. Algae\|Daphnia system was constructed based on a controlled density of algae, at which Chlorella pyrenoidosa had great capacity of uptaking phosphorus. Algae\|Daphnia system can work well by controlling the amount ratio of Chlorella/Daphnia at 302×106∶1, when algae density was between 0.928×106 millions and 1.60×106 millions cells/mL.

Key words: eutrophication, Chlorella pyrenoidosa, Daphnia magna, bio\, manipulation, phosphorus removal

摘要：

微藻对水体有明显的净化作用，探讨了在开放条件下蛋白核小球藻及蛋白核小球藻-大型溞修复系统对污水中磷的去除效果。研究表明接种8 h蛋白核小球藻对磷达到过饱和吸收，过饱和吸收量是（3.12±039）×10-10 mg/cell；接种量1.00×105 cells/mL～3.72×106 cells/mL无菌培养96 h对总磷的去除率达到75%；正常生长过程中藻细胞除磷量（饱和含磷量）为（1.46±0.05）×10-10 mg/cell；在每日补充营养盐的开放环境下，72 h～216 h藻细胞除磷量（饱和含磷量）稳定在（1.50±0.50）×10-10 mg/cell，微藻系统稳定运转时除磷量为（006±0.01）mg/（L·d），此时藻密度为1.11×106～3.79×106 cells/mL。藻-溞修复系统表明，藻密度在928×105～1.60×106 cells/mL范围内，藻溞数量比为3.02×106∶1时藻－溞修复系统能稳定运行。

关键词: 富营养化；蛋白核小球藻；大型溞；生物操纵；除磷