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    15 February 2014, Volume 16 Issue 1
    Challenges and Countermeasures for the Development of Biomass Energy Industry in China
    JIA Jing\|dun1§, SUN Kang\|tai1§, JIANG Da\|hua1,2, WEI Xun1, ZHANG Hui1, QI Wei
    2014, 16(1):  1-6.  DOI: 10.13304/j.nykjdb.2013.641
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    Energy shortage and environment crisis are faced all around the world as a result of over exploitation and use of fossil fuels. Countries in the world have paid attentions to exploit clean and renewable energy, which is strategically important for China’s economic structure transition. With the development of biomass energy industry, energy shortage is hoped to be relieved, meanwhile, environmental protection and greenhouse gas reduction could also be improved due to the bidirectional cleaning effect of biomass energy. This paper deeply analyzed the strategic significance and opportunities/challenges of biomass energy industry in China, countermeasures and policy suggestions for the development of biomass energy industry were also proposed in this paper.

    Research Progress on Plant Secondary Metabolite Bioreactor
    TANG Ke\|xuan, SHEN Qian, FU Xue\|qing, YAN Ting\|xiang
    2014, 16(1):  7-15.  DOI: 10.13304/j.nykjdb.2014.028
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    Plant secondary metabolite bioreactor is one kind of plant bioreactors. Its aim is to improve the quantity of useful metabolites of plants by genetic modification of plant secondary metabolic pathway through metabolic regulation and metabolic engineering on the molecular level. Along with the more plant secondary metabolic pathways being elucidated, the wider research and application prospects plant secondary metabolite bioreactor has. In this article, the research progresses and prospects in plant secondary metabolite bioreactor were reviewed and summarized using terpenes and alkaloids as examples.

    Status and Prospect of Agrobiotechnology\|based Breeding in China
    KANG Le1, WANG Hai\|yang2*
    2014, 16(1):  16-23.  DOI: 10.13304/j.nykjdb.2014.029
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    Agrobiotechnology\|based breeding technologies have revolutionized the modern agriculture and significantly impacted the society, economics and ecology on a globe scale. Nevertheless, traditional phenotype\|based breeding is still the dominant method used in crop breeding in china nowadays, which can not meet the demand of our rapid social and economic development. Agrobiotechnology\|based breeding can break down the barriers and bottlenecks of traditional breeding and facilitate the transition from “empirical breeding” to “accurate breeding”, with increased efficiency and being more controllable and predictable. This article reviewed the recent progress made with four agrobiotechnology based breeding approaches, including transgenic\|based breeding, molecular marker\|assisted selection breeding, breeding by molecular design and the third\|generation hybrid technology in rice and maize, two major crops in China.

    Receat Progress and Trend of Molecular Breeding in Beef Cattle
    GUO Hong1, LV Yang2, CHEN Jian\|xing2,3, LI Guang\|peng2*
    2014, 16(1):  24-31.  DOI: 10.13304/j.nykjdb.2014.027
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    Molecular breeding is a new subject for livestock improvement, and it has played a role in provoting the studies on cattle meat quality traits and growth and development. This paper overviewed the latest research achievements of molecular marker and molecular breeding in beef cattle; and introduced the research progress made in beef cattle growth and production traits, including beef cattle candidate molecular markers, genome\|wide selection, micro\|satellite markers related with production performance, noncoding RNA as new markers for molecular breeding and adopting transgenic technology to improve important production traits of beef cattle.

    Advance in Genome Editing Technologies for Livestock Improvement
    WEI Jing\|liang, WU Tian\|wen, RUAN Jin\|xue, MU Yu\|lian*
    2014, 16(1):  32-38.  DOI: 10.13304/j.nykjdb.2014.003
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    Genome editing is a novel technology which enables targeted biological genome modification. It can boost the transgenic efficiency, realize knockout livestock gene and fix targeted integration. Recently, zinc finger nucleases(ZFN), transcription activator\|like effector nucleases(TALEN)and bacterial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immunity system, have been used to cleave the double\|strand DNA at specific site, followed by endogenous DNA repair, which can lead to homologous recombination repair and non\|homologous end joining. These techniques, therefore, make precise changes to the DNA of living cell by knocking out a gene, fixing targeted integration or replacing DNA fragment at the specific site. Many breakthroughs have been achieved recently using this technology, especially in the improvement of certain important production traits, such as meat quality, growth rate and disease resistance, etc. on pigs, cattle, sheep and other livestock. Genome editing technology makes it possible to efficiently improve livestock on fixed point of genes, which will definitely exert a profound impact on the development of industrialization of transgenic animal.

    Progress and Prospect of Plant Immunity Inducer
    QIU De\|wen
    2014, 16(1):  39-45.  DOI: 10.13304/j.nykjdb.2014.043
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    Plant immune inducers to regulate the metabolism of plants and plant disease can enhance the resilience of the immune system by activating plant. Development of pesticide plant immune inducers in controling crop diseases is new idea and new way for plant protection. This article described the development process of the plant immune inducers at home and abroad. Plant immunity inducers registration status of production. By analyzing the development and application of inducing agent, this practice prospected and analyzed the application prospect and development trend of inducers.

    Comparison of Protective Efficacy among Different Types of H5 Subtype Avian Influenza Vaccines
    ZHAO Shuang\|cheng1,2, LIU Jin\|xiong1,2, CHEN Pu\|cheng1,2, ZENG Xian\|ying1,2,
    2014, 16(1):  46-51.  DOI: 10.13304/j.nykjdb.2014.025
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    To evaluate the protective efficacies of four different types of avian influenza vaccines, including the reassortant avian influenza virus vaccine, inactived (H5N1 subtype, strain Re\|1), recombinant fowlpox virus vector\|based live vaccine(H5 subtype), avian influenza and newcastle disease recombinant vaccine, live(Strain rLH5\|1)and avian influenza DNA vaccine(H5 Subtype, pH5\|GD), which were all constructed on the bases of the first H5N1 isolate A/Goose/Guangdong/1/1996(H5N1) \[GS/GD/96\]. Groups of 3\|week\|old specific pathogen free (SPF) chickens were intramuscularly injected (2.8 μg HA/0.3 mL), via wing\|web puncture (103PFU/100 μL), oculonasal administration (106EID50/100 μL) and intramuscularly injected (15 μg/200 μL) with four different types of H5 avian influenza vaccines, respectively. Boosts were conducted with the same dosage after three weeks. All chickens were nasally challenged with 106EID50 of highly pathogenic avian influenza virus (HPAIV) GS/GD/96 at two weeks after the boost. Oropharyngeal and cloacal swabs were collected from all groups at 3 d, 5 d and 7 d post the challenge for titration of virus in embryo eggs, and the chickens were observed daily for disease signs and deaths for 2 weeks. Meanwhile, sera were collected weekly after vaccination and challenge to detect the dynamics of the hemagglutinin inhibition (HI) antibodies. Complete protections, indicated by no disease signs, no death, and no virus shedding, were monitored in all vaccinated groups. The average HI titers induced by the four vaccines were 875log2, 6.5log2, 5.13log2 and 7.88log2 before challenge, respectively. Our results indicated that all the vaccines were able to induce highly protective efficacies in SPF chickens and have potentials of H5N1 vaccine candidates in pratice.

    Advance in Mechanism of Brassinosteroids in |Plant Development
    ZHENG Jie, WANG Lei*
    2014, 16(1):  52-58.  DOI: 10.13304/j.nykjdb.2013.254
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    Brassinosteroids(BRs), an important steroidal hormone, are involved in multiple development progresses in plants. Combining the genetics, genomics, proteomics and cell biology, important achievements on BRs regulation mechanism have been made in plants. This paper summarized the current research progresses of BRs on plant cell division, cell elongation, reproductive development, photomorphosis, stress response and tropism development, which were expected to provide reference for researchers.

    Effects of Nitrogen Fertilizer on Activities of Key Enzymes of Carbon and Nitrogen Metabolism of Different Flue\|cured Tobacco Varieties
    GE Guo\|feng1,2, WANG Shu\|hui2, LIU Wei\|qun1*
    2014, 16(1):  59-64.  DOI: 10.13304/j.nykjdb.2013.172
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    To study the effects of nitrogen fertilizer on activities of key enzymes of carbon and nitrogen metabolism of different varieties of flue\|cured tobacco, this paper carried out field experiment and determined the changes in activities of nitrate reductase (NR), glutamine synthetase (GS), glutamate dehydrogenase (GDH), sucrose phosphate synthase (SPS), sucrose synthase (SS) of 2 varieties of flue\|cured tobacco, Honghuadajinyuan (H) and K326 (K), under circumstances of N supplied and  without N supplied. The results showed that the applying nitrogen fertilizer for the same variety can increase activities of NR, GS at the early growth stage of flue\|cured tobacco; and the activity of GDH at the middle growth stage. At the same time, N supplying can increase the activity of SPS activity at early stage and activity of SS at the late stage. Under the same N supplying cases, activities of NR in K326 and SPS are higher than that in Honghuadajinyuan at early stage, so as the GDH activity in K326 during carbon metabolism period. Activity of GS in Honghuadajinyuan is higher than that in K326 at late growth stage. Nitrogen fertilizer can stimulate the nitrogen metabolism of different tobacco plant varieties. K326 utilizes nitrogen more than Honghuadajinyuan at the early stage, while at the late stage K326s reuse of nitrogen is less than Honghuadajinyuan. Therefore, in the process of flue\|cured tobacco growth and development, adequate supply of nitrogen fertilizer should be ensured according to nitrogen demands of different flue\|cured tobacco varieties.

    Isolation and Characterization of a New Pseudomonas aeruginosa Strain from Bacterial Stalk Rotted Maize
    YANG Li\|ping1,2, YANG Gen\|hua1, LI Zhi\|lin1, XIE Hua2*, MA Rong\|cai2*
    2014, 16(1):  65-70.  DOI: 10.13304/j.nykjdb.2013.152
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    To identify the pathogen of maize bacterial stalk rot disease occurred in Zhengzhou, Henan Province, the strain YM02 was isolated from maize stalk rot samples and then identified and characterized by morphology observation, pathogenicity test, biochemical and physiological characteristics analyses, and molecular phylogeny genetic relationship analysis. The results showed that the isolated strain (YM02) was a Gram\|negative pathogenic bacterium; it could ferment several kinds of sugar or alcohol to produce acid, and transfer arabinogalactan into alkali. In addition, the 16S rDNA sequence of YM02 strain had a similarity of above 99% with those of internal and external isolated Pseudomonas aeruginosa strains. Thus, this strain was named as Pseudomonas aeruginosa YM02, which had the closest genetic relationship with Pseudomonas aeruginosa strains ANSC、S164S、S167S(2) and strains CS\|2. The isolation and characterization of this pathogen had provided basic data for the prevention and treatment of maize bacterial stalk rot disease.

    Effects of Maltose Transport Related Gene Expression on Avermectins Production in Streptomyces avermitilis
    LI Meng1, ZHANG Xuan2, WEN Ying2*, SONG Yuan2
    2014, 16(1):  71-75.  DOI: 10.13304/j.nykjdb.2013.181
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    Avermectin is a macrolide compound produced through fermentation by S.avermitilis, and its a kind of widely used biological pesticide. This paper investigated the function of maltose transporter system and the relation between the expression of maltose transporter system and the yield of avermectins in S.avermitilis. The malEFG gene was amplified by PCR using genomic DNA of S. avermitilis as template, and then was inserted into multi\|copy vector and integration vector, respectively. Each expression vector was transformed into S. avermitilis ATCC31267 and GB\|165, respectively. The resulting transformants were cultured in fermentation medium to study the effect of malEFG overexpression on avermectin biosynthesis. The yield of avermectin was increased by 3~3.2 fold in ATCC31267 and by 40%~60% in GB\|165 after the malEFG gene being overexpressed in them compared to that of parent strain.

    Functional Analysis of ktrA Gene Encoding Potassium Uptake Protein from Deinococcus radiodurans
    XIAN Xian\|yi1,2, JIANG Shi\|jie1,2, CUI Guang\|yan1,2, LIU Ying\|ying2, DAI Qi\
    2014, 16(1):  76-81.  DOI: 10.13304/j.nykjdb.2013.464
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    The Deinococcus radiodurans R1 potassium uptake protein encoded by ktrA gene(DR\|166), played an important role under salt and osmotic stress. The quantitative real\|time PCR assay showed that the expression of ktrA gene was up\|regulated remarkably under salt and D\|sorbitol in wild strain. Construction of D. radiodurans R1 mutants deleted ktrA gene (ΔktrA) by means of fusion PCR and homologous genetic technology. Survival rates of the ΔktrA obtained and wild strain were investigated under different concentrations of KCl and D\|Sorbitol. The results indicated that the ΔktrA were much more sensitive to KCl and D\|Sorbitol treatment comparing to that of wild type, which indicated KtrA protein played an important role when influenced by salt and osmotic stress.This research will be helpful to study the functions of ktrA gene.

    Research Progress on Rapeseed Crop Model
    ZHANG Wei\|xin1,2, CAO Hong\|xin2*, ZHU Yan1*, LIU Yan2,
    2014, 16(1):  82-90.  DOI: 10.13304/j.nykjdb.2012.441
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    Crop model is an important content in agricultural model, which is mainly divided into crop growth model and crop morphological structure model. This paper reviewed the internal and external research progress on these two rapeseed models, including rapeseed development, growth (photosynthesis, respiration, and distribution), water balance, nitrogen balance, organ formation, and management, etc. The paper analyzed the research status and existing problems and also prospected of the future development of rapeseed crop models.

    Construction and Analysis of Cotton Seed Quality and Safety Traceability System Based on RFID
    LI Yong\|bin1,2, ZHANG Li\|xin1*, HU Rong1
    2014, 16(1):  91-97.  DOI: 10.13304/j.nykjdb.2013.209
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    According to the problems existing in products quality traceability system from cotton seed processing to market circulation in China, combined with cotton processing technology, and based on RFID radio frequency identification technology, this paper considered about content of cotton seed quality and safety information in every link. analyzed the reading and writing part of hardware structure and software program to further explain how to implement the cotton seed RFDI label data transfer, and designed an architecture about the cotton quality safety traceability system based on RFID. The paper also analyzed the feasibility, reliability and profitability of this system. Consumers can consult and monitor cotton processing production and sale information through network on real\|time, get cotton quality and safety rank information,and effectively put an end to shoddy cotton into the market, eventually realizes the aim of cotton quality safety traceability management.

    Yield Formation of Huaimai 25 with High Yield and Wide Adaptability
    SUN Su\|yang, WANG Yong\|jun, LI Hai\|jun, LI Li\|li
    2014, 16(1):  98-103.  DOI: 10.13304/j.nykjdb.2013.584
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    Huaimai 25 is a new wheat cultivar with high yield and wide adaptability released by Huaiyin District Institute of Agricultural Sciences of Jiangsu Province. This paper analyzed its yield formation and stability by the data from many years′ national and regional tests in order to provide reference for the current high yield wheat breeding. The results showed that Huaimai 25 achieved extreme significant yield increase for 2 successive years in southern Huang\|Huai Winter Wheat Regional Test. During the 3 years′ test, the average productive ear numbers were 648×104/hm2, kernels per spike were 37, and per 1 000\|grain weight was about 38 g. The 3 yield factors of this cultivar are well coordinated. Huaimai 25 has wide and strong adaptability. Both its dynamic and static stability are good.

    Effect of Plant Growth Regulator and Foliar Fertilizer on Preventing Premature Aging of Foxtail Millet Hybrid
    XIA Xue\|yan1, CHENG Ru\|hong1*, CHEN Yuan1, SHI Zhi\|gang1, ZHANG Ting1,
    2014, 16(1):  104-110.  DOI: 10.13304/j.nykjdb.2013.387
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    To solve the problem of premature aging of hybrid millet blades in filling stage, this paper started with plant growth regulator and foliar fertilizer, took self\|bred hybrid 66359 as test material, and studied the influence of preventing premature aging of different plant growth regulators and fertilizers on agronomic traits, dry matter weight and some physiological indexes through determination of main agronomic traits and chlorophyll SPAD value and its net photosynthetic rate in filling stage after spraying 12 kinds of growth regulator and 3 kinds of foliar fertilization and water control. This study also selected suitable plant growth regulator and foliar fertilizer with effect of preventing premature aging. The results showed that genwang, hailvsu, shifeng, tiancibao had effect of preventing premature aging for hybrid millet. Its physiological effects were shown as increasing blade chlorophyll content, improving photosynthetic rate, adjusting assimilate transportation and distribution, enhancing dry matter accumulation of each vegetative organs, promoting the root system development, strengthening the stem, prolonging the function period of blade, and preventing the leaf premature aging.

    Distribution Characteristics of Phosphorus Content in Flue\|cured Tobacco and its Relationship with Conventional Chemical Components
    LIU Chao1, GENG Zong\|ze2, YANG Jie2, HUANG Wu\|xing1, SHAO Hui\|fang1, XU Zi\|c
    2014, 16(1):  111-116.  DOI: 10.13304/j.nykjdb.2013.258
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    This paper studied the distribution characteristics of phosphorus contents of 107 flue\|cured tobacco leaf samples from domestic main tobacco areas and Zimbabwe, and analyzed the relationships between phosphorus contents and routine chemical components. The results indicated that: ① the phosphorus content of domestic main tobacco areas ranged from 0.25% to 0.66% with the mean of (0.43±0.08)%, the phosphorus content of Zimbabwe ranged from 0.45% to 0.67% with the mean of (0.53±0.05)%; ② the order of the phosphorus contents in flue\|cured tobacco leaves among different areas was Zimbabwe (0.53%) > Southeast tobacco area (0.49%) > the upper and middle Yangtze River tobacco area (0.45%) > Southwest tobacco area (0.42%) > the Yellow and Huai River tobacco area (0.41%) > North tobacco area (0.39%), and the differences among these areas reached extremely significant level; ③ the phosphorus contents increased with the rise of stalk position, and there was no significant difference among different positions; ④ the phosphorus content in flue\|cured tobacco leaf had significant or extremely significant positive correlation with nicotine, potassium and chlorine contents, and had extremely negative correlation with total sugar, reducing sugar content and nitrogen/nicotine, sugar/nicotine, but had no significant correlation with total nitrogen and potassium/chlorine; ⑤ the clustering results based on the phosphorus contents and harmonic character evaluation of the chemical components at different phosphorus content levels showed that the flue\|cured tobacco leaves with medium and high phosphorus content clusters had proper chemical components, better harmony among indexes and high available value.

    Research Progress on Plant Vegetative Growth and Protection under Ozone Pollution Stress
    LI Shuo1, ZHENG You\|fei1,2*, WU Rong\|jun1, YIN Ji\|fu2, XU Jing\|xin2
    2014, 16(1):  117-124.  DOI: 10.13304/j.nykjdb.2013.201
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    Recently, the areas with high concentration of ozone are ever\|increasing, and the studies on ozone effects on plants are growing in depth. Studies are developed from the most basic ozone damage phenomenon to ozone damage mechanism, and to ozone damage effect on plant in vivo elements. Thus, these studies have provided the basis for developing scientific agricultural management measures and ensuring high and stable crop yield. This paper summarized the research progress on the effect of ozone concentration rising on crop in vivo nutrient elements at home and abroad, and also on plant ozone protection. It has provided theoretical reliance to scientific fertilizing, rational cultivation, and to ensure grain safety. The paper also pointed out several aspects for further study in ozone pollution research field in China, so as to promote studies on plant nutrition under atmospheric environment change condition.

    Damage of Reclamation on Grassland Slope in Farming pastoral Interleaving Areas, North Yinshan Mountains
    GAO Tianming1, ZHANG Ruiqiang1*, HUANG Jianguo2
    2014, 16(1):  125-130.  DOI: 10.13304/j.nykjdb.2012.416
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    In the majority of North Yinshan Mountains, the lowlying land in farmingpastoral interleaving areas has been reclaimed, due to the increase of population, grassland slope was reclaimed exhaustedly. Recently, many grassland slopes became arable lands. The ecological environment deterioration became more and more serious. Taking Saiwusu of Damao County and Baotou City of Inner Mongolia as example, this paper studied the effect of reclamation on erosion, vegetation and soil characteristics. The results indicated that severe wind erosion in spring and severe water erosion in summer eroded the soil of slopping fields; reclamation has brought remarkable changes in vegetation, annual herb such as Salsols collina, Corispermum declinatum, Chenopodum aristatum, Artimisia annua et al. grew vigorously, while perennial herb such as Stipa krylovii, Leymus chinensis and Agropyron cristatum were inhibited; soil fertility declined sharply, due to the erosion and harvest. In the first year after the grassland converted to slopping field soil erosion increased over 5 times, soil fertility droped 30%, and vegetation changed completely. After 4 years reclamation, soil fertility droped 50%, vegetation became sparse, crop yield was low, the slopping fields were no longer fit to continue farming, and large areas were abandoned. Due to sparse vegetation, low soil fertility and intense erosion, the abandoned slopping fields caused not only serious disturbances on the local ecological environment, but also became a dust storm source, thus brought serious threat to the ecological safety of Northern China. Therefore, protecting basic grassland, strictly controlling grassland reclamation is extremely imperative.

    Dynamic Changes in Cultivated Land Resource Security and Driving Forces in Heibei Province during the Past 20 Years
    LI Chao1, ZHANG Xiang\|yi2, ZHANG Hai\|tao3, ZHANG Li2,WANG Shu\|tao2*
    2014, 16(1):  131-138.  DOI: 10.13304/j.nykjdb.2013.292
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    The cultivated land resource safety is of great significance for ensuring regional grain security. Taking relevant cultivated land data from 1990 to 2010 in Hebei Province as basis, this paper built a dynamic change evaluation system for cultivated land resources security. The quantitative evaluation was carried out on the security status of cultivated land resource by coefficient of entropy and TOPSIS model, and the principal component analysis method was adopted to analyze its driving forces. The result showed that aggregative index of cultivated land resources security temperal changed obviously between the state of insecurity and unsafe condition during the past 20 years. The index of quantity security was first decreased and then increased, the index of quality security showed clear growth trend; the index of ecological security showed a declining trend year by year. The driving factors affecting the security of cultivated land resources were socio\|economic factors, demographic factors, and natural factors. Before ending, the paper put forward related counter\|measures to improve the safety of the cultivated land resources in Hebei Province.

    Genetic Diversity of Different Geographic Populations in Perinereis aibuhitensis by ISSR and COI Markers
    WANG Yi\|xiao, REN Hong\|wei, ZHU Ying\|jun, ZHAO Huan, YANG Da\|zuo, ZHOU Yi\|b
    2014, 16(1):  139-147.  DOI: 10.13304/j.nykjdb.2013.106
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    ISSR molecular markers and CO I gene sequence were used to identify the genetic diversity and structure of 7 geographic populations in Perinereis aibuhitensis, which were sampled from Dalian(DL), Rushan(RS), Panjin(PJ), Qingdao(QD), Yancheng(YC), Jinjiang(JJ)and west coast of North Korea(CX). The ISSR results showed that there was high genetic diversity between the 7 geographic populations of P. aibuhitensis. 116 bands were amplified with 10 ISSR primers with 109 polymorphic bands. The total percentage of polymorphic loci was 93.97%. Neis gene diversity was 0.365 2, Shannons information index was 0.518 6. The coefficient of gene differentiation (Gst) was 0246 0. These data indicated that among\|population component accounted for 24.60% of the total variation, while the within\|population component accounted for 76.40%. The within\|population genetic variation was larger than among\|population. COI gene sequence was analyzed employing 70 individuals from 7 populations. There were 68 variable sites and 47 parsimony\|information sites out of the total 469 sites. In 68 variable sites, there were 62 transition sites and 6 transversion sites, parsimony\|information sites rate Pi was 9.611%. Phylogenetic tree analysis showed that there was high genetic diversity within the tested population, which was accord with the result of ISSR. This result has provided theoretical basis for effective utilization and protection of P. aibuhitensis resources.

    Phosphorus Removal of Eutrophic Water by Algae\|Daphnia System
    MA Jin\|feng, DENG Chun\|sheng*, ZHANG Guang\|ke, ZHANG Yan\|rong, WANG Ni\|shan
    2014, 16(1):  148-156.  DOI: 10.13304/j.nykjdb.2013.255
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    Microalgae has obvious effect on water purification, this paper explored phosphorus removal capacity of Chlorella pyrenoidosa in open system and purification effect of wastewater by Algae\|Daphnia system. It was indicated that after incubated in sterile wastewater for 8 h, algae can achieve max amount of phosphorus absorption, (2.99±045)×10-10 mg/cell. After incubated in sterile wastewater for 96 h, the rate of phosphorus removal reached to 75%, when inoculation quantity of algae was between 1.00×105 cells/mL and 3.72×106 cells/mL. The phosphorus removal capacity of each multiplied algae cell(saturated phosphorus content) was (1.46±0.05)×10-10 mg/cell in the sterile wastewater, and it was(1.50±0.50)×10-10 mg/cell in the open system from 72 h to 216 h, under the condition that nutrition was added to the system every day. The amount of phosphorus removal was (0.06±0.01)mg/(L·d), when the system worked well and algaes density was between 1.11×106 and 3.79×106 cells/mL. Algae\|Daphnia system was constructed based on a controlled density of algae, at which Chlorella pyrenoidosa had great capacity of uptaking phosphorus. Algae\|Daphnia system can work well by controlling the amount ratio of Chlorella/Daphnia at 302×106∶1, when algae density was between 0.928×106 millions and 1.60×106 millions cells/mL.

    Studies on Technique of Producing Glucose from Sweet Potato Residue by Enzymes
    WU Yun\|shan1, GUO Jun2, YAN Gong\|hong3, WEI Dong\|wang1*, GE Fu\|xiang1,
    2014, 16(1):  157-162.  DOI: 10.13304/j.nykjdb.2013.240
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    Sweet potato residue is the by\|product of sweet potato starch production. It contains about 50% starch. In this study, the alpha\|amylase, glucoamylase, and cellulase were used to hydrolyze the sweet potato residue, and then were inactivated by the thermo treatment. The sugar products were separated and concentrated. Analyzed by the high performance liquid chromatography (HPLC), most component of the product is glucose. The condition of enzyme hydrolysis was optimized with the orthogonal experiment, and the results are as follows: the material content is 6 percent, the amount of amylase is 12 U/g dry matter, liquefaction time is 60 min, the amount of glucoamylase is 300 U/g dry matter, the amount of cellulase is 0.5 U/g dry matter, saccharification time is 24 h. Because of its simple craft and its product with good quality and high quantity, this technique can help to solve serious environmental pollution caused by sweet potato residue, may have good industrial applications prospects.

    Empirical Analysis of Factors on Farmers Income and Differences in China ——Based on the Income Decision Equation and Shapley Value Decomposition
    SONG Li\|li1, WANG Xiu\|dong1, LIU Xu2*
    2014, 16(1):  163-171.  DOI: 10.13304/j.nykjdb.2013.539
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    Since the reform and opening up, Chinas economic development has made great achievements, but it did not benefit all. The income gap among the famers expanded continuously, which not only exacerbated the problem of rural poverty and weakened the income growth of farmers, but also seriously affect the harmony and stability for the sustainable development of Chinese economy and society. This paper firstly analyzed by income decision equation cross\|section data collected from 566 households of sample survey in Jiangsu, Henan and Sichuan Provinces and determined the major factors affecting the farmer households incomes. Secondly, it analyzed the contribution of each significant factor on the income differences between households by Shapley value decomposition. The results showed that the average outdoor\|work time of household labor force during a year was the most important factor affecting households income increase and differences in between. And the average education years of labors took the second place. Based on the above mentioned results, this paper suggested that speeding up the development of non\|agricultural industries in rural  areas, increasing the non\|agricultural employment opportunities, adding financial input to rural education, and providing equal access to education and training to farmers will  sustainably increase farmers incomes, and reduce the differences between farmers incomes at the same time.

    Impact of Information Technology Application on Farmers Income
    LI Xiang, XU Wei\|ping, GUO Yong\|tian*, RAO Xiao\|yan, YAN Dong\|hao, ZHANG Hua
    2014, 16(1):  172-181.  DOI: 10.13304/j.nykjdb.2013.604
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    To study the impact of information technology application on farmer households income from agricultural business, this paper collected and surveyed 1 072 valid questionnaires from 6 provinces and cities. By establishing multi\|line regression model analysis, the paper found that ① the agricultural informationization level needed to be improved; ② at present the traditional elements have greater impact on farmer households income than informationization elements; ③ the income of farmer households who search for agriculture information through varies channels is 45.8% higher than those who never search for information; ④ in western regions more foundational information infrastructure and primary information technology promotion are needed; ⑤ the effect for households living in central and eastern China to improve conditions of agricultural production by using information technology is better. Based on the above mentioned analysis the paper put forward some policy suggestions.