Most Read articles

    Published in last 1 year |  In last 2 years |  In last 3 years |  All

    All
    Please wait a minute...
    For Selected: Toggle Thumbnails
    Development Status of Pre-prepared Poultry Meat Dishes Industry, Influencing Factors and Promotion Strategies of Its Quality and Safety
    Shenghai HUANG, Xiujun TANG, Xiaoxu JIA, Lina MA, Yinyin LIU, Yushi GAO
    Journal of Agricultural Science and Technology    2024, 26 (6): 133-140.   DOI: 10.13304/j.nykjdb.2023.0543
    Abstract1467)   HTML1)    PDF (519KB)(239)       Save

    Pre-prepared poultry meat dishes plays an important role in improving people’s living standards, rural revitalization, standardization of the catering industry and high-quality development of the poultry industry. However, there are many unfavorable factors affecting the quality and safety during the production and consumption processes of pre-prepared poultry meat dishes. The factors that affect quality and safety in various aspects such as poultry breeding and slaughtering, processing and packaging, storage and transportation, sales and consumption of pre-prepared poultry meat dishes were analyzed. According to the establishment of poultry ecological breeding environment and input management, the construction of poultry slaughtering site and production management, the optimization of pre-prepared poultry meat dishes processing technology and the implementation of green label system, the selection of packaging materials and the application of packaging technology, the establishment of scientific management system and the application of information technology in storage and transportation, the improvement of sales management system and scientific guidance to consumers and so on, corresponding optimization strategies were proposed aiming to promote the improvements of quality and safety and the development of the pre-prepared poultry meat dishes industry, which provided a theoretical basis and model reference for rural revitalization, the solution of the problem of ‘agriculture, rural areas and farmers’, the development of poultry industry and catering industry, the needs of the people for a better life and the construction of ecological civilization.

    Reference | Related Articles | Metrics
    Research Progress on Biocontrol Mechanism and Synergistic Disease Prevention of Trichoderma
    Xiao WEI, Chunxia CAO, Daye HUANG, Jingwu YAO, Qinfeng YUAN
    Journal of Agricultural Science and Technology    2024, 26 (11): 126-135.   DOI: 10.13304/j.nykjdb.2023.0524
    Abstract1289)   HTML4)    PDF (551KB)(44)       Save

    Trichoderma is a widely distributed filamentous fungus and widely used as a biological fungicide to control a variety of plant diseases in agriculture due to its excellent biocontrol potential. Trichoderma can reduce plant diseases caused by pathogens through different direct mechanisms (mycoparasitism and the production of cell wall degrading enzymes, antibiosis, competition for space or nutrients) or indirect mechanisms (induction of plant defense ). Compound biocontrol agents have received increasing attention in recent years. Trichoderma can be used in combination with other biocontrol products to produce better biocontrol effects because of its resistance to chemical pesticides and compatibility with rhizosphere microorganisms. The related mechanisms of Trichoderma, including parasitism, antibiosis, nutrient competition and induction of plant resistance were summarized, and the current research progress were discussed. In addition, the research progress of synergistic disease prevention between Trichoderma and other microorganisms or non-biological (chemical fungicides, other substances) with disease prevention effect was reviewed, and the current research status and the subsequent development prospects of Trichoderma as a biological fungicide were explored.

    Table and Figures | Reference | Related Articles | Metrics
    Identification and Biological Characteristics of a Colletotrichum fructicola Causing Chestnut Internal Rot Disease
    Shifeng MU, Xiaolei WEN, Lina FENG, Dexuan ZHAO, Suhong GAO, Peng GAO, Huixia QI
    Journal of Agricultural Science and Technology    2024, 26 (9): 122-128.   DOI: 10.13304/j.nykjdb.2023.0871
    Abstract1281)   HTML4)    PDF (1614KB)(33)       Save

    Chestnut internal rot is the main disease of chestnut during postharvest storage, which causes rot and mildew of chestnut kernel and brings huge economic losses to production. In order to clarify the pathogenic bacteria and its biological characteristics, the strain ZHZF21 was obtained by tissue isolation method. The strain was identified by morphological characteristics and ITSTUB2 and CAL multi-gene sequence analysis. The pathogenicity of strain ZHZF21 was determined by mycelial inoculation method, and its biological characteristics were studied. The results showed that the colony of strain ZHZF21 was dark green ring concentric circles, with sexual and asexual reproduction stages. This strain was clustered with Colletotrichum fructicola numbered NDSTY31 in GenBank based on multi-gene sequences. Combined with colony morphological characteristics and molecular tree, strain ZHZF21 was preliminarily identified as Colletotrichum fructicola. The brown lesions after inoculation of strain ZHZF21 were consistent with the natural symptoms. The strain ZHZF21 could grow at 5~35 ℃, the optimum temperature was 25 ℃, the lethal temperature was 45~50 ℃, and the optimum pH was 6. Under the condition of full darkness, strain ZHZF21 grew fastest, the utilization rates of arabinose and yeast were the highest, the utilization rate of lactose was the lowest, and urea could hardly be used.

    Table and Figures | Reference | Related Articles | Metrics
    Progress of Research on Alleviating Obstacles of Continuous Cropping by Soil Sterilization and Arbuscular Mycorrhizal Fungi
    Yaping FAN, Baiquan SONG, Changxian WANG
    Journal of Agricultural Science and Technology    2024, 26 (10): 158-167.   DOI: 10.13304/j.nykjdb.2023.0386
    Abstract1253)   HTML4)    PDF (562KB)(39)       Save

    The continuous cropping obstacles have been widespread in horticultural production, and the reasons were complicated. It has done serious harm to the ecosystem consisting of soil, plants and rhizosphere microorganisms. The economical and convenient soil sterilization technologies effectively alleviate the obstacles, among the sterilization technologies, high temperature sterilization and reductive soil disinfestation improve soil environment. Meanwhile, symbiotic associations between arbuscular mycorrhizal (AM) fungi and plant root systems is helpful to reconstruction of rhizosphere ecosystems. Focused on the continuous cropping obstacles, this paper reviewed the effect of high temperature sterilization, reductive soil disinfestation and arbuscular mycorrhizal fungi on the obstacles alleviation and their mechanisms. Furthermore, meaningful research works in this field were prospected. The review provided a theoretical and technical basis for the alleviation of continuous cropping obstacles in horticultural production.

    Table and Figures | Reference | Related Articles | Metrics
    Effect of Different Storage Temperatures on Quality of Jingcai 1 Watermelon
    Lanting XIANG, Shuhui SONG, Lijuan LIU, Xiaoling SHE, Jiahua ZHOU, Baogang WANG, Hong CHANG, Chao ZHANG, Daqi FU, Yunxiang WANG
    Journal of Agricultural Science and Technology    2024, 26 (9): 137-145.   DOI: 10.13304/j.nykjdb.2023.0685
    Abstract1242)   HTML0)    PDF (1639KB)(28)       Save

    To explore the effect of storage temperature on Jingcai 1 watermelon, the fruits of Jingcai 1 watermelon were storaged under 5, 15 and 25 ℃. The firmness, rate of weight loss, chilling injury, soluble solids, pectin, cellulose, soluble sugar, organic acid, lycopene, β?-carotene and vitamin C contents were determined under different temperature treatments. The results showed that, with the storage extending, the weight loss rate of Jingcai 1 watermelon fruit increased, the fruit firmness decreased, the content of water-soluble pectin (WSP) increased, the sodium carbonate-soluble pectin (SSP) decreased, the vitamin C, lycopene and β?-carotene showed decreasing trend, the variation of soluble solids fluctuated within a small rage, the cellulose decreased at first and then increased, while the content of soluble sugar and organic acid showed different changing trends at different storage temperatures. When stored at 5 ℃, the vitamin C and soluble sugar of fruit could be high level, but a long-term storage would raise the chilling injury rate and cause gradual wastage of nutrients, resulting undoubtedly in loss of the edible and commercial values. When stored at 15 ℃, the contents of water, organic acids and cytochromes could be better maintained, giving watermelons better quality and flavor. When stored at 25 ℃, the quick loss of soluble sugar, organic acid and pectin of the fruit leaded to soft rot, depriving original flavors of watermelons and making it difficult to maintain its quality. Above results provided a theoretical basis for the commercialization of its storage, transportation and preservation.

    Table and Figures | Reference | Related Articles | Metrics
    Study on Insecticidal Activity Constituents and Mechanism of Artemisia annua Against Mythimna separate
    Chunyan LI, Qian LIANG, Chengxiang GAO, Zongsuo LIANG, Fang WANGA
    Journal of Agricultural Science and Technology    2024, 26 (12): 129-137.   DOI: 10.13304/j.nykjdb.2023.0589
    Abstract1198)   HTML2)    PDF (1636KB)(51)       Save

    In order to study the insecticidal activity components and preliminary mechanism of the petroleum ether extract from Artemisia annua against Mythimna separata, the insecticidal activity of different fractions and main components were measured by insect immersion method. The petroleum ether extract was separated by column chromatography and analyzed by gas chromatography-mass spectrometry to determine its chemical components, and the target enzyme and metabolic enzyme activities in the body of Mythimna separata were measured for the active components. The results showed that the petroleum ether extract of Artemisia annua was separated by silica gel column chromatography, and 27 fractions were obtained, of which fraction 8 showed the best contact toxicity. According to gas chromatography-mass spectrometry, the main compounds were tridecane, 4-quinolinecarboxaldehyde, caryophyllene oxide, linoleic acid ethyl ester, linolenic acid ethyl ester with contents of 7.9%, 72.8%, 3.6%, 2.4%, 3.1%,respectively. The lethal concentration of 50% (LC50) of above 5 compounds on the 7th day were 7.9, 2.5, 3.6, 5.4 and 1.5 mg·mL-1, respectively, which indicated that ethyl linolenic acid had significant contact toxicity against M. separata. On the 7th day of treatment with 1.5 mg·mL-1 linolenic acid ethyl ester, the scanning electron microscopy results showed that the wax layer structure in the surface of M. separata were loosely, the wax layer particles disappeared significantly, and the wax flower gap increased. And the acetylcholinesterase (AChE) and carboxylesterase (CarE) of M. separata treated with 1.5 mg·mL-1 linolenic acid ethyl ester showed first activation and then inhibition, Na+-K+-ATPase showed inhibition over time, and catalase (CAT) showed first inhibition and then activation. To sum up, the linolenic acid ethyl ester was the main active compound of A. annuawhich provided reference for the development of new plant-based insecticides and biological control of M. separata and the development and utilization of A.annua resources.

    Table and Figures | Reference | Related Articles | Metrics
    Evaluation of the Growth-promoting Effect of Trichoderma harzianum on Tobacco and Its Induced Resistance to Black Shank Disease
    Junjia CHANG, Jiaxin GAI, Gang TAO, Zhuanlonghai MO
    Journal of Agricultural Science and Technology    2024, 26 (10): 168-176.   DOI: 10.13304/j.nykjdb.2024.0225
    Abstract1131)   HTML3)    PDF (2248KB)(40)       Save

    To investigate the growth-promoting effects of Trichoderma harzianum on tobacco and its induced resistance mechanisms against tobacco black shank disease caused by Phytophthora nicotianae, 5 Trichoderma strains were screened. The results showed that the strain T. harzianum ACCC 32808 (TH32808) was demonstrated the strongest inhibition (62.00%) among 5 strains of Trichoderma. The growth-promoting effects of TH32808 on different tobacco varieties and its induced resistance against tobacco black shank disease were assessed. The results showed that, under pot condition, TH32808 exhibited different ability on promoting the growth of different tobacco varieties. Under TH32808 treatment, the fresh weight of Honghuadajinyuan increased by 8.30%, the control efficacy against black shank disease in Honghuadajinyuan was 51.74%. In Honghuadajinyuan and K326, the catalase activity peaked on the 4th day, reaching 1.42 and 2.15 times that of CK, the peroxidase activity peaked on the 2nd day, being 2.49 and 2.29 times that of CK, the superoxide dismutase activity peaked on the 4th day, reaching 1.17 and 1.15 times that of CK, respectively. Moreover, TH32808 could induce resistance against black shank disease, key genes involved in the metabolic pathways of salicylic acid, jasmonic acid and ethylene, such as NPR1PDF1.2EFE and NtSGT1, all exhibited an upregulation trend. Above results provided fungal resources and theoretical basis for the biological control and induced resistance research against black shank disease in tobacco.

    Table and Figures | Reference | Related Articles | Metrics
    Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides
    Ying CHEN, Xiaofeng LIU, Yiping CHEN, Liping LIAO, Hongtao FU
    Journal of Agricultural Science and Technology    2024, 26 (12): 122-128.   DOI: 10.13304/j.nykjdb.2023.0247
    Abstract1128)   HTML1)    PDF (1444KB)(19)       Save

    To establish the ultraviolet-visible (UV-VIS) spectral library to quickly and accurately retrieve the hidden components added in the pesticide fungicide, with acetonitrile and water as the mobile phase, the UV-VIS spectrograms of 98 conventional bactericide standards were separated and extracted by gradient elution using a high-performance liquid chromatograph, and the UV-VIS spectrograms of the standard substances were used to establish the UV-VIS spectrogram library. The target compound was matched according to the spectral similarity and the retention time of the standard sample, so as to retrieve the hidden components added in the pesticide preparation. 2 bactericides were used to verify the accuracy of the spectral library retrieval, and the results showed that the accuracy of the spectral library retrieval reached 100%. This method had the advantages of high accuracy, easy operation and low cost.

    Table and Figures | Reference | Related Articles | Metrics
    Study on Control Effect of Streptomyces phaeoluteichromatogenes HEBRC45958 Strain on Corynespora Leaf Spot of Tomato
    Daye HUANG, Zhibin YU, Zhongyi WAN, Dan YANG, Jinping LI, Chunxia CAO
    Journal of Agricultural Science and Technology    2024, 26 (11): 136-142.   DOI: 10.13304/j.nykjdb.2023.0416
    Abstract1123)   HTML1)    PDF (3075KB)(22)       Save

    In order to clarify the control effect of Streptomyces phaeoluteichromatogenes strain HEBRC45958 against Corynespora leaf spot of tomato, the antifungal activity was determined in vitro by using dual culture assay combined with microscope and scanning electron microscope observation. The siderophore production and the activities of hydrolases were also determined. The control effect of the fermentation broth of HEBRC45958 strain on Corynespora leaf spot of tomato was determined in pots. The results showed that the inhibition rate HEBRC45958 on Corynespora cassiicola was 52.65% in the dual culture. Compared with control, the mycelium treated with HEBRC45958 strain was distorted and cracked by scanning microscopy, and the space between the cell wall and cell membrane was widened by transmission electron microscopy. The HEBRC45958 strain could produce amylase, cellulase, chitinase and siderophore. The control effect of fermentation broth against Corynespora leaf spot of tomato was 100% in pot experiment, which showed that HEBRC45958 strain had a good prospect of biocontrol application for Corynespora leaf spot of tomato.

    Table and Figures | Reference | Related Articles | Metrics
    Effect of Exogenous NO on Expression of Tomato Antioxidant Enzyme Gene Under Tomato Yellowing Leaf Curl Virus Stress
    Jincheng LUO, Xiaolin ZHU, Xiaohong WEI, Xian WANG, Baoqiang WANG, Xuefen DU
    Journal of Agricultural Science and Technology    2025, 27 (2): 125-135.   DOI: 10.13304/j.nykjdb.2023.0647
    Abstract1113)   HTML2)    PDF (2999KB)(19)       Save

    In order to investigate the effect of exogenous nitric oxide (NO) on the expression of tomato antioxidant gene under the stress of tomato yellow leaf curl virus (TYLCV), the susceptible tomato Jinpeng 1 was used as the experimental material. Transcriptome sequencing, fluorescent qRT-PCR and bioinformatics analysis were performed under control (CK), TYLCV (TY) and NO+TYLCV (NO+TY) treatments. The results showed that a total of 55 antioxidase-coding genes were selected in the tomato genome, among which the antioxidase-containing genes with the highest number of exons in different subcellular regions were significantly responsive to TYLCV stress. The number of NO-mediated antioxidant enzyme coding genes in different subcellular compartms showed chloroplast>cell membrane>cytoplasm>peroxisome>vacuole, in which the expression levels of Chl Cu-Zn SODChl MR2Chl GRPer MRPla CAT1 and Pla CAT7 were significantly up-regulated, and the expression levels of Chl Fe SOD1Chl Fe SOD2Cyt GPXCyt APX1Cyt APX2 L-5Pla CAT3Pla CAT8 and Vac CAT were significantly down-regulated. Fluorescence qRT-PCR verified that Chl GRMin Mn SOD and Per CAT2 responded to TYLCV, and Chl Cu-Zn SODPla CAT7Pla CAT8 and Cyt APX2 L-5 responded to TYLCV and NO. Above results provided theoretical basis for studying the mechanism of NO in improving tomato disease resistance.

    Table and Figures | Reference | Related Articles | Metrics
    Fruit Quality Analysis of Prunus domestica × armeniaca at Different Harvest Maturity Levels
    Ruyue WANG, Haifang HU, Shasha LUO, Ziyi ZHEN, Yeyong XU, Xiaojing HU
    Journal of Agricultural Science and Technology    2025, 27 (2): 158-169.   DOI: 10.13304/j.nykjdb.2023.0429
    Abstract1108)   HTML5)    PDF (3014KB)(35)       Save

    To study the effects of different harvest maturity levels on the quality of P. domestica × armeniaca fruits, and to screen out the suitable maturity levels for picking P. domestica × armeniaca,using P. domestica × armeniaca ‘Fengweihuanghou’ as the test materials, the changes in indicators such as single fruit weight, fruit color and fruit hardness and so on under different maturity levels(Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ) were analyzed by variance, correlation and principal component analysis. The results showed that with the increase of harvest maturity levels, the red green value (a*) of the fruit skin, the red green value (a*) of the fruit flesh, the blue yellow value (b*) of the skin, the blue yellow value (b*) of the flesh, the comprehensive ratio of the skin (a*/b*) and the comprehensive ratio of the flesh (a*/b*) all showed a significant upward trend, which increased by 15.811, 15.760, 23.009, 15.380, 0.973 and 0.558, respectively, during the entire maturity and development period, and were highly significant positive correlation with fruit maturity. Chlorophyll a+b, peel brightness value (L*)and flesh brightness value (L*) showed significant downward trend, with decrease of 17.230 μg·cm-3, 11.460 and 13.492 during maturity, respectively. The single fruit weight, soluble solids, solid to acid ratio and membrane permeability all showed a significant upward trend with the increase of harvest maturity level. At maturity Ⅴ, the various indicators were 101.500 g, 18.780%, 85.535% and 62.050%, respectively, and showed a highly significant correlation with maturity level. The skin hardness, peel hardness and titratable acid showed a significant downward trend with the delay of maturity, reaching their lowest values at maturity Ⅴ, which were 13.770 N, 6.886 N and 0.246%, respectively, which were significantly negatively correlated with harvest maturity level. The principal component analysis showed that the fruit quality of different maturity level was as follows: maturity Ⅴ>maturity Ⅳ>maturity Ⅲ>maturity Ⅱ>maturity Ⅰ. Based on production needs and comprehensive economic benefits, maturity Ⅰ and Ⅱ were not suitable for harvesting,and maturity Ⅳ and Ⅴ had good quality and taste, but the fruit softens severely. Maturity Ⅲ could be selected as the appropriate harvesting maturity. Above results could provide theoretical basis and scientific basis for the subsequent research and production of P. domestica × armeniaca .

    Table and Figures | Reference | Related Articles | Metrics
    Effects of Bacillus thuringiensi on Intestinal Bacteria in Ectropis obliqua
    Ruoheng JIN, Xiaoyu LI, Jingwu YAO, Beibei WANG, Chunxia CAO, Daye HUANG
    Journal of Agricultural Science and Technology    2025, 27 (2): 141-149.   DOI: 10.13304/j.nykjdb.2023.0794
    Abstract1053)   HTML1)    PDF (3110KB)(17)       Save

    Ectropis obliqua is one of the most important pests on tea, which has brought serious economic losses to the tea industry. Bacillus thuringiensis (Bt)?, as a key biological insecticide for the control of E. obliqua, plays an important role in the green prevention and control of diseases and pests in tea gardens. To clarify the effect of Bt treatment on intestinal bacteria of E. obliqua larvae, the Illumina platform using 16S rDNA high-throughput sequencing was applied to compare the structure and population diversity of intestinal commensal community in E. obliqua larvae treated with different dosage of Bt suspensions. The results showed that, compared with CK treatment, the intestinal bacterial composition of E. obliqua larvae treated with lower dosage of Bt did not change significantly, but those treated with higher dosage of Bt had significant effect on the composition of intestinal bacteria in E. obliqua larvae. And the treatments with higher dosage of Bt significantly increased the diversity and abundance of intestinal bacteria in E. obliqua larvae, which indicated that intestinal microorganisms were involved in the response of E. obliqua larvae to Bt infection. Above results provided basis for further exploring the insecticidal mechanism of Bt, and provided new ideas for improving the virulence of Bt.

    Table and Figures | Reference | Related Articles | Metrics
    Effect of Glycosylation on Quality and Volatile Flavor Characteristics of rainbow trout ( Oncorhynchus mykiss) Floss
    Lei JI, Tianhong LIU, Ying WANG, Xiao LI, Hongyan LI, Xiaodong JIANG, Yuanqin SUN, Shuaizhong ZHANG
    Journal of Agricultural Science and Technology    2024, 26 (9): 159-172.   DOI: 10.13304/j.nykjdb.2023.0731
    Abstract998)   HTML0)    PDF (1304KB)(17)       Save

    To promote the development of high-value products for rainbow trout (Oncorhynchus mykiss), the quality (physicochemical properties, color, and sensory properties) of rainbow trout floss with different sugars added were investigated. At the same time, the volatile components and fatty acids of the floss were analyzed by gas chromatography-mass spectrometer (GC-MS). The results showed that the addition of sugar had an important impact on the quality of rainbow trout floss, which could promote the process of Maillard reaction and improve the sensory quality of fish floss such as taste and color. As pentose, the fish floss with arabinose had a faster process of Maillard reaction and more obvious color change, while the fish floss with fructooligosaccharides had a better sweet taste. In terms of fatty acid, the contents of saturated fat acid, eicosapntemacnioc acid (EPA) and docosahexaenoic acid (DHA) of the fish floss with oligofructose were the highest, and the content of polyunsaturated fatty acid of the fish floss with arabinose was the highest. In conclustion, due to Maillard reaction, the fish floss added with sugar produced aldehydes, ketones, alcohols and other substances with lower threshold, which could impact the flavor. Above results provided reference for improving the quality of rainbow trout floss and increasing product added value.

    Table and Figures | Reference | Related Articles | Metrics
    Symptoms and Pathogen Identification of Ficus carica Bacterial Leaf Spot Disease in Fujian
    Mengting JI, Ling ZHU, Xiaohua LUO, Yuting ZHENG, Shun XIAO, Fangping HU, Xueqing CAI
    Journal of Agricultural Science and Technology    2025, 27 (1): 147-154.   DOI: 10.13304/j.nykjdb.2023.0546
    Abstract972)   HTML0)    PDF (2145KB)(206)       Save

    To clarify the taxonomy of the pathogen causing a new bacterial leaf spot disease on fig (Ficus carica) in Fuzhou in 2020, diseased fig leaves were collected as test materials. The bacterial strains were isolated from the diseased leaves by streak plate method, and then the pathogenicity of the strains was determined by tobacco allergic reaction, spray inoculated fig leaves and Koch’s rule. The pathogen was identified based on morphological characteristics, physiological and biochemical determination, and phylogenetic tree analysis using partial sequences of 16S rDNA, gyrB and rpoD. The results showed that 10 bacterial strains were isolated and purified from the diseased leaves, healthy fig leaves demonstrated similar symptoms as the specimen collected from fields, and the identical morphological bacterial strains were re-isolated from diseased fig leaves. Thus, all of the bacterial strains were identified as the causal pathogens of bacterial leaf spot disease by Koch’s rule. The colony morphology was light yellow, round, and flat on the NA medium after 2 d of culture, and the gram staining reaction was negative. The bacterial strains could produce green water-soluble fluorescent on KB medium. The sequencing analysis based on 16S rDNA, gyrB and rpoD showed that the test strains were clustered with Pseudomonas cichorii; and the results of physiological and biochemical characteristics, Biolog phenotypes instrument, and specific primers were similar to the P. cichorii strain 5707, therefore the 10 isolated bacterial strains were identified as P. cichorii. It was the first report that P. cichorii could naturally cause the leaf spot disease in F. carica.

    Table and Figures | Reference | Related Articles | Metrics
    Preliminary Function Analysis of GhERF020 Gene in Response to Verticillium Wilt in Cotton
    Huiting WENG, Haiyang LIU, Huiming GUO, Hongmei CHENG, Jun LI, Chao ZHANG, Xiaofeng SU
    Journal of Agricultural Science and Technology    2024, 26 (9): 112-121.   DOI: 10.13304/j.nykjdb.2023.0407
    Abstract951)   HTML3)    PDF (3709KB)(26)       Save

    Verticillium wilt (VW) is a disruptive symptom caused by Verticillium dahliae, which affects fiber yield and quality of cotton in China. AP2/ERF transcription factors play prominent role in plant growth, development and stress response. In order to excavate the genes related to resistance to VW in cotton and explore their biological functions, the candidate gene GhERF020 (XM_016842745.1) was obtained by homologous alignment of AT1G22810.1 related gene of Arabidopsis thaliana against V. dahliae. Bioinformatic analysis showed that the open reading frame length of GhERF020 was 441 bp, encoded 147 amino acids. GhERF020 contained a conserved AP2 domain, the relative molecular weight was 16.16 kD without transmembrane structure, belonging to the hydrophilic protein. It was predicted that the promoter region contained response elements of salicylic acid (SA), methyl jasmonate (MEJA), abscisic acid (ABA) and other response elements. The expression of GhERF020 was induced by V. dahliae infection. Subcellular localization analysis showed that GhERF020 protein was localized in the nucleus. As the expression of GhERF020 was downregulated by virus-induced gene silencing (VIGS) technique, the susceptibility of cotton plants to V. dahliae was significantly enhanced, and the disease index and pathogen biomass were distinctly increased. Above results indicated that GhERF020 was a positive regulator in resistance against V. dahliae in cotton.

    Table and Figures | Reference | Related Articles | Metrics
    Research on Processing Technology and Shelf Life of Red Matsutake Beef Sauce
    Lijun FU, Xiaoyu LIN, Jianhua LIN, Huinan SHEN, Yongzhen WU
    Journal of Agricultural Science and Technology    2024, 26 (6): 148-158.   DOI: 10.13304/j.nykjdb.2022.0975
    Abstract948)   HTML0)    PDF (4519KB)(113)       Save

    To optimize the optimal formula of red matsutake beef sauce, the response surface methodology was used to study the effects of the additions of red matsutake, soybean paste, millet pepper and potato starch on quality of red matsutake beef sauce. The shelf life was predicted by Arrhenius equation and kinetic equation based on peroxide value and acid value. The results showed that the optimal formula of red matsutake beef sauce obtained by response surface methodology was followed with 15.0% red matsutake, 37.4% soybean paste, 13.4% millet pepper, and 8.3% potato starch, the sensory score of red matsutake beef sauce was 91.23 under these conditions. Under different ambient temperatures (25, 37 and 45 ℃), the total viable count, peroxide value and acid value of red matsutake beef sauce were positively correlated with storage time, while the sensory score showed a downward trend. The shelf life of red matsutake beef sauce was predicted to be 147 d at 25 ℃ based on peroxide value, and was 103 d at 25 ℃ based on acid value, so the shelf life model constructed based on acid value was more reliable. Above results provided theoretical support for high value utilization and shelf life prediction of red matsutake.

    Table and Figures | Reference | Related Articles | Metrics
    Effects of Astragalus Polysaccharides on Production Performance, Carcass Performance and Meat Quality of Stellar-fed Tan Sheep
    Guoshun CHEN, Bin TIAN, Yancheng GAO, Yancui LI, Wenjin TIAN, Wenjing YANG, Wancheng WU, Lijun HUANG
    Journal of Agricultural Science and Technology    2025, 27 (2): 150-157.   DOI: 10.13304/j.nykjdb.2023.0541
    Abstract919)   HTML3)    PDF (497KB)(24)       Save

    To elucidate the effects of astragalus polysaccharides on the growth, slaughtering performance and meat quality of Tan sheep, 24 healthy, similar-weight (22.17±1.21 kg) 50-days-old Tan lambs were randomly divided into 4 groups for fattening trials, including basal diet group (control group), basal diet+0.05% astragalus polysaccharide group (test group Ⅰ), basal diet+0.10% astragalus polysaccharide group (test group Ⅱ) and basal diet+0.15% astragalus polysaccharide group (test group Ⅲ). The weights were determied on 0, 10 and 70 d of the trial, and the slaughter performance and meat quality were analyzed on at the end of the trial (70 d), respectively. The results showed that, compared with control group, the final body weight (FBW) and average daily gain (ADG) of test group Ⅱ were significantly increased, and the feed conversion rate was significantly decreased. The carcass weight and slaughter rate of test group Ⅱ increased significantly, the cooked meat rate of test group Ⅱ and test group Ⅲ increased significantly. The back fat thickness of test group Ⅰ and test group Ⅱ treatment was significantly reduced, and the L* value of test group Ⅱ was significantly reduced. The contents of glycine, aspartic acid and total amino acids in muscle of Tan sheep treated with test group Ⅱ and test group Ⅲ were significantly increased, and the content of umami amino acids was significantly increased in treatments with astragalus polysaccharides. The contents of monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids in mutton of Tan sheep treated with test group Ⅱ were significantly increased. And the value of MUFA/TFA (total fatty acids) of test group Ⅱ and test group Ⅲ increased significantly. In summary, the addition of 0.10% astragalus polysaccharides to the diet could improve the growth performance, carcass performance and meat quality of Tan sheep.

    Table and Figures | Reference | Related Articles | Metrics
    Physiological Response of Tobacco Infected by Phytophthora to Trichoderma harzianum Inoculation Sequence
    Zhichao REN, Yaohui MU, Xuyang YAO, Shue LI, Yongfeng ZHANG, Tianbao REN, Guoshun LIU, Quanyu YIN
    Journal of Agricultural Science and Technology    2025, 27 (1): 155-164.   DOI: 10.13304/j.nykjdb.2023.0544
    Abstract904)   HTML0)    PDF (1598KB)(15)       Save

    To explore the physiological response of tobacco plant infected by Phytophthora to Trichoderma harzianum application sequence, the pot experiments were carried out with different treatments including inoculated with Phytophthora spore suspension (T1), inoculated with T. harzianum spore suspension (T2), inoculated with Phytophthora spore suspension first and then inoculated with T. harzianum spore suspension (T3), inoculated with T.harzianum spore suspension first and then inoculated with Phytophthora spore suspension (T4), inoculated with Phytophthora spore suspension and T. harzianum spore suspension at the same time (T5), and clear water (CK) as controls. The effects of T. harzianum application sequence on biological characteristics, physiological characteristics and induced resistance of tobacco infected by Phytophthora were analyzed. The results showed that, in the growth of tobacco plants, the agronomic characters of tobacco plants with T4 treatment increased the most, and the dry and fresh weight of underground parts increased by 103.73% and 8.30% compared with CK. In the physiological characteristics of tobacco plants, T. harzianum could reduce the inhibition of Phytophthora on photosynthetic pigment accumulation and root activity of tobacco plants, and significantly increase both of them, and it could also strengthen the activities of enzymes related to carbon and nitrogen metabolism in tobacco plants. After transplanting 21 d, the activities of nitrate reductase and glutamine synthetase with T4 treatment increased by 13.14% and 6.40%, respectively. In the control effect, T4 treatment had the lowest incidence and disease index, which were 39.39% and 13.89%, and the highest antioxidant enzyme activity. In conclusion, there were significant differences in the physiological response of tobacco plants to the inoculation sequence of T. harzianum. In production, T. harzianum should be inoculated in advance to promote the growth and development of tobacco and inhibit the incidence of black shank disease. Above results laid a theoretical foundation for the regulation of tobacco growth and development and the comprehensive prevention and control of black shank.

    Table and Figures | Reference | Related Articles | Metrics
    Comparative Analysis of Honey from Different Sources Based on Multidimensional Indicators
    Ming ZHANG, Miaomiao CHE, Xinli HUO, Xue WANG, Xing ZHENG, Leilei ZHAO, Yazhou ZHAO
    Journal of Agricultural Science and Technology    2025, 27 (2): 170-179.   DOI: 10.13304/j.nykjdb.2023.0522
    Abstract870)   HTML1)    PDF (4114KB)(21)       Save

    Honey is a natural product with complex composition and characteristics, which vary depending on the botanical source, origin, bee species and so on. In order to evaluate, differentiate and identify honey samples, a comparative analysis of honey from different sources using multidimensional indicators, including conventional physicochemical analysis and high performance liquid chromatography-quadrupole-time of flight/mass spectrometer (HPLC-Q-TOF/MS) technique were conducted. The results showed significant differences in amylase activity, fructose, glucose, phenolic acid and flavonoid contents among different honey samples. Among them, rape honey exhibited the lowest amylase activity with a value of (3.42±0.70) mL·g-1·h-1, while acacia honey (Qinshui) had the highest amylase activity with a value of (12.66±2.44) mL·g-1·h-1. The total phenolic acid and total flavonoid contents of Apis cerana honey were higher than other honey samples. Pro-His-Cys and hydroxytyrosol 1-O-glucoside might be useful in distinguishing honey from nectar plant species. Palustrine played an important role in differentiating acacia honey from different regions. Above results provided methodological guidance for the characterization and authenticity assessment of honey, that was, the multidimensional index method could be used as a valuable tool for comparative analysis of honey samples from different sources.

    Table and Figures | Reference | Related Articles | Metrics
    Flavor Differences of Different Types of Fresh Corn Based on Electronic Nose and GC-IMS Technology
    Yaxing SHI, Hui DONG, Li XU, Yanli FAN, Hui LIU, Yamin SHI, Ainian YU, Ning GAO, Jiuran ZHAO, Baishan LU, Ronghuan WANG
    Journal of Agricultural Science and Technology    2024, 26 (11): 143-156.   DOI: 10.13304/j.nykjdb.2024.0708
    Abstract738)   HTML0)    PDF (2974KB)(30)       Save

    Flavor is an important indicator of the edible quality of fresh corn. In order to explore the composition and content differences of volatile flavor compounds in different types of fresh corn,in this study, 6 different types of fresh corn varieties including NKN 323 were used as materials, and electronic nose technology and gas chromatography ion mobility spectrometry (GC-IMS) technology were used to detect the volatile flavor compounds in fresh corn to evaluate the contribution of volatile flavor compounds to the overall flavor of fresh corn by relative odor activity value (ROAV). The results showed that a total of 34 volatile flavor compounds were identified, including 10 esters (34.68%~26.74%), 8 aldehydes (28.46%~21.22%), 6 alcohols (10.33%~6.04%), 5 ketones (14.74%~9.87%), 2 ethers (10.05%~6.94%) and 3 other compounds. GC-IMS fingerprints showed that the difference of volatile flavor compounds content important which caused the difference of characteristic flavor of different types of fresh corn. 5 common key aroma components and 23 different characteristic flavor compounds of fresh corn were identified by ROAV. The total release of volatile flavor compounds of different types of varieties was NKN323, followed by JKT608.Isovaleraldehyde contributed the most to the overall flavor of NKN336, JZN219, JKN768 and JKT608, while 3-octanone contributed the most to the overall flavor of NKN323 and JKN2000. Above results provided theoretical basis for the analysis and evaluation of volatile flavor compounds in fresh corn and the precise control of characteristic flavor.

    Table and Figures | Reference | Related Articles | Metrics
    Effect of Chinese Chestnut Yellow Crinkle Disease on Quality of Chestnut
    Dexuan ZHO, Peng GAO, Xiaolei WEN, Shifeng MU, Suhong GAO, Lina FENG, Weiming SUN, Huixia QI
    Journal of Agricultural Science and Technology    2025, 27 (2): 136-140.   DOI: 10.13304/j.nykjdb.2023.0844
    Abstract727)   HTML1)    PDF (559KB)(10)       Save

    In order to comprehensively evaluate the effect of Chinese chestnut yellow crinkle disease on the quality of chestnut nuts, the related indexes of nut quality of trees were determined, and the effect of the disease on nut quality was analyzed. The results showed that, compared with healthy trees, the contents of trace elements in nut of diseased trees were increased, and the contents of amino acids, total phenols and flavonoids were significantly increased by 24.93%, 20.54% and 36.33%, respectively. The chestnut bracts of diseased trees had long and sof spines, uneven color of nuts, the wrinkled surface and the rough texture. The number of grains per bract, the weight of single bract, the full nut rate and the yield per plant of diseased trees were significantly reduced, among them, the yield per plant was reduced by 96.45% compared with the healthy tree. Above results provided a theoretical basis for scientific evaluation of the effect of Chinese chestnut yellow crinkle disease on nut quality.

    Table and Figures | Reference | Related Articles | Metrics
    Impact of ExsA on the Pathogenicity of Aeromonas veronii
    Zihui CAI, Wenxin CHEN, Juanjuan LI, Hong LI, Xue CHI, Yanqiong TANG, Zhu LIU, Xiang MA
    Journal of Agricultural Science and Technology    2024, 26 (9): 129-136.   DOI: 10.13304/j.nykjdb.2024.0316
    Abstract692)   HTML0)    PDF (1704KB)(15)       Save

    Aeromonas veronii is an important pathogen in the co-occurrence of humans and fish, causing significant mortality in various aquatic organisms, posing a major threat to aquaculture and public health care. The type Ⅲ secretion system (T3SS) is one of the most critical virulence mechanisms in determining the pathogenicity of Aeromonas species. The transcription factor ExsA of AraC family serves as the main regulatory factor and activates T3SS assembly and effector protein secretion in response to changes in environmental conditions. In order to explore the effect of exsA on the pathogenicity of the A. veronii, this study constructed an exsA gene knockout strain using homologous recombination strategy and tested its biofilm formation, adhesion capabilities, as well as the cytotoxicity of the spent cultural supernatant. The results showed that the deletion of the exsA gene in A. veronii led to a significant decrease of its biofilm formation capability, its adhesion to ex vivo host tissues, and the cytotoxicity of the spent culture supernatants. Compared with the wild type, the ability of the exsA knockout strain to form biofilm was reduced by 1.5 folds. The adhesion to epithelial cells was not significantly changed in exsA knockout strain as compared with the wild type, while the adhesion to ex vivo mouse intestinal tissues decreased by 3.4 folds and the cytotoxicity of bacterial culture supernatants decreases by 1.2 folds. These results proved that exsA plays an important regulatory role in the virulence mechanism of bacterial pathogen, laying a solid foundation for further research on the function of T3SS in the pathogenic mechanism of A. veronii and provided clues for establishing strategies for the control of the aquatic infection.

    Table and Figures | Reference | Related Articles | Metrics
    Optimization of Extraction Conditions for Volatile Components in Jujube Honey Based on Plackett-Burman Design and Response Surface Method
    Xue WANG, Xing ZHENG, Jiamiao REN, Yazhou ZHAO, Wenjun PENG
    Journal of Agricultural Science and Technology    2025, 27 (1): 181-192.   DOI: 10.13304/j.nykjdb.2023.0384
    Abstract638)   HTML0)    PDF (3482KB)(86)       Save

    In order to enhance the head space-solid phase micro extraction (HS-SPME) extraction efficiency of volatile components in honey, and for more accurate qualitative and quantitative analysis using gas chromatograph-mass spectrometer (GC-MS), the total peak area of volatile components of the chromatogram in Ziziphus jujuba Mill honey was used as the index. Plaekett-Burman design and response surface methodology were used to optimize extraction conditions (extraction temperature, extraction time, sample amount, deionized water amount, NaCl additive amount, stirring speed). The results showed that extraction temperature, extraction time and sample amount significantly impacted the total peak area in the chromatograms. The optimized extraction conditions were extraction temperature of 68.5 ℃, extraction time of 51.6 min and sample amount of 5.1 g. Under the optimal extraction conditions, the total peak area of chromatogram could reach 2.20×109 mAU·min-1. Above results provided certain technical guidance for the further profile analysis of volatile components in honey.

    Table and Figures | Reference | Related Articles | Metrics
    Identification and Sensitivity to Fungicides of Pathogen Species of Hass Avocado Fruit Anthracnose
    Jinlin LIU, Yingying CHEN, Xiaomei LAN, Haiyong CHEN, Pengsheng LI, Bi QIN, Ye YANG
    Journal of Agricultural Science and Technology    2025, 27 (1): 138-146.   DOI: 10.13304/j.nykjdb.2023.0702
    Abstract636)   HTML1)    PDF (3306KB)(17)       Save

    To identify the pathogen of anthracnose in avocado and screen the suitable fungicides, fruit samples of Hass avocado suspected anthracnose disease from Danzhou city, Hainan province were sampled. And the pathogen was isolated and identified. The pathogenicity of strain was confirmed according to Koch’s postulate. And the sensitivity of pathogenic strain to 9 fungicides were evaluated in vitro using mycelium radial growth assay. The results showed that the 4 pathogenic strains were identified as Colletotrichum siamense according to the morphological and phylogenetic analyses of multi-locus sequences. All of 4 strains showed virulence and high pathogenicity to avocado fruits. Among 9 fungicides, prochloraz was the most effective with the mean effective inhibitory medium concentration (EC50) values of 0.11 mg·L-1, and azoxystrobin, difenoconazole, pyrazoxystrobin, penthiopyrad and carbendazim followed with mean EC50 values ranged from 2.09 to 4.29 mg·L-1. So these 6 above-mentioned fungicides exhibited strong inhibitory activities against the mycelial growth of C. siamense, which could be used as candidate agents for the control of avocado anthracnose. Above results should help in developing strategies for the management and control of anthracnose in avocado.

    Table and Figures | Reference | Related Articles | Metrics
    Whole Genome Sequencing of Multi-drug Resistant Escherichia coli and Its Drug Resistance Analysis
    Haili LI, Yindi XU, Zhifang WANG, Wenhao ZHU, Lixian ZHANG, Chunjiang MA
    Journal of Agricultural Science and Technology    2024, 26 (6): 113-121.   DOI: 10.13304/j.nykjdb.2023.0169
    Abstract496)   HTML4)    PDF (4555KB)(895)       Save

    In order to understand colistin resistance genes carried by Escherichia coli, screen sensitive plant drugs, and solve the dilemma of multiple drug resistance and no drug option in animal clinic, the resistance genes of Escherichia coli were identified by 16S rRNA sequencing and PCR. The antimicrobial susceptibility of 78 antibiotics were detected, and the bacteriostatic and bactericidal effects of 4 kinds of natural plant extracts (palmatine, berberine, baicalin and macleaya cordata) were tested. The results showed that 145 strains of E.coli were isolated and identified from intestinal tract of swine clinical diarrhea cases in 2021 (from January to December) and 2022 (from January to June), and a clinical strain carrying colistin resistance genes (mcr-4,mcr-5) and β-lactamases blaTEM and AmpC was identified, which named HN2149. The antimicrobial susceptibility of 78 antibiotics showed that the HN2149 strains was sensitive to cefepime, cefodizime, fosfomycin, cefixime, meropenem, cefoxitin, cefazolin, cefoperazone, cefotaxime, ceftazidime, cefuroxime, ceftriaxone, aztreonam, piperacillin-tazobactam, ticacillin/clavulanic acid, cefoperazone sulbactam, ceftazidime/clavulanic acid, cefotaxime/clavulanic acid, ceftizoxime, cefmetazole, cefetamet, and was resistant to 57 antibiotics. The drug sensitivity of 4 plant extracts showed that the macleaya cordata had the best bacteriostatic and bactericidal effect on HN2149 strain, while the other 3 extracts had no effects on HN2149 strain. Above results provided reference for the prevention and control of swine colibacillosis.

    Table and Figures | Reference | Related Articles | Metrics
    Differential Expression Paradigm of Chemoreceptor Genes Between Males and Females at Different Developmental Stages of Carposina sasakii Matsumura
    Qian ZHANG, Lina MEN, Yiran LI, Qiao LIU, Angie DENG, Xiaowen HU, Yuhong ZHANG, Zhiwei ZHANG, Wei ZHANG
    Journal of Agricultural Science and Technology    2024, 26 (8): 151-162.   DOI: 10.13304/j.nykjdb.2023.0338
    Abstract417)   HTML1)    PDF (2139KB)(36)       Save

    Carposina sasakii Matsumura is a notable fruit pest that damages many types of fruit trees. Behavior regulation technology has gradually gained attraction as a potential pest control method that is both environmentally conscious and effective. The insect behavioral mechanism of chemosensory perception is the basis of behavior regulation. A total of 242 olfactory genes were identified in the antenna transcriptome of the C. sasakii. Based on sequence alignment and functional background of similar genes in related species, 13 olfactory genes were selected and their potential recognition functions were analyzed using RT-qPCR. The results showed that 6 genes differentially expressioned between males and females at different developmental stages ( P<0.05), which corresponded to the behavioral phenotypes of adults. According to the difference expression levels between male and female adults during newly emerged, mature unmated and mature mated, OBP11 might play a role in detecting pheromones and mating behavior, OR45 might correspond to host-seeking and oviposition activities and IR5 was associated with mating and oviposition behavior. Above results laid theoretical molecular foundation for the identification of C. sasakii pheromone and olfactory-related genes and supported the implementation of behavioral regulators against the olfactory perception mechanism of C. sasakii as a more environmentally sustainable pest control strategy.

    Table and Figures | Reference | Related Articles | Metrics
    Inhibition Mechanism of Water Extract from Spent Mushroom Substrateagainst Phytophthora infestans in potatoes
    Wenjing MO, Hongsen CHEN, Fangze GUI, Ciqing HONG, Xinkai CAI, Xiong GUAN, Xiaohong PAN
    Journal of Agricultural Science and Technology    2024, 26 (5): 129-137.   DOI: 10.13304/j.nykjdb.2023.0159
    Abstract414)   HTML0)    PDF (3149KB)(42)       Save

    Mushroom substrate is the residue of mushroom production, which is rich in inorganic salts and organic matter. The water extract from spent mushroom substrate(WESMS) of Lentinula edodes was prepared by hot water extraction,the ultraviolet (UV) absorption, surface charge and particle size distribution were characterized using an UV spectrophotometer, Zeta potential and particle size analyzer. The inhibition rate of WESMS against Phytophthora infestans was calculated by plate penetration and cross diameter measurement. Further, both optical microscopy and scanning electron microscopy were used to observe the damage caused by WESMS to the morphology of the pathogen cells. Subsequently, the impact of WESMS on the DNA of Phytophthora infestans was analyzed by agarose gel electrophoresis. The results showed that WESMS could absorb UV rays at A (UVA, 315~400 nm), B (UVB, 280~315 nm) and C (UVC, 100~280 nm) bands. Moreover, the particle size distribution in water was 3 649.27 nm. The antibacterial experiment showed that WESMS had an inhibitory effect on the growth of pathogens. The inhibitory effect became more obvious with the increase of the volume percentage concentration of WESMS. The inhibition rate of WESMS against pathogens was nearly 100% at a high volume concentration of 6.25%. The mycelia of pathogenic cells treated with WESMS exhibited obvious distortion, flatness and wrinkles compared with the control group, which indicated that WESMS could cause severe damage to cells. In addition, the DNA band brightness of the treatment group was darker than that of the control group, and the degree of DNA damage was positively related to the volume percentage concentration of WESMS. Above results provided scientific basis and technical support for the utilization of spent mushroom substrate and its use in the effective control of potato late blight.

    Table and Figures | Reference | Related Articles | Metrics
    Bactericidal Mechanism of Antimicrobial Peptide PAJE Against Staphylococcus aureus and SCVs
    Siqi GAO, Xinjian YANG, Deqi ZHU, Mingwei GUAN, Yunting KOU, Cheng MAN, Jian JIAO
    Journal of Agricultural Science and Technology    2024, 26 (5): 156-166.   DOI: 10.13304/j.nykjdb.2023.0878
    Abstract407)   HTML1)    PDF (2850KB)(41)       Save

    Staphylococcus aureusS. aureus) and the small colony variants (SCVs) infection are considered as main issues affect livestock and poultry health. However, there are still many challenges in persistent and recurrent infections, and even drug resistance for treatment of traditional antibiotics.The antimicrobial peptide PAJE has the broad-spectrum antibacterial activity. To study the mechanisms of peptide PAJE against S. aureus and SCVs, the antibacterial activity, stability and biological characteristics were determined. The results indicated that the antibacterial peptide PAJE had a penetration ability to destruct bacterial cell wall, even permeated 54.7% and 62.9% cell membrane. Additionally, the peptide PAJE could also up-regulate the Agr regulatory system genes, down-regulate the SigB regulatory system and biofilm forming related genes, which greatly inhibited biofilm formation. In addition, higher temperature and pH stability made PAJE better processing, storage, and environmental tolerance. In conclusion, the antimicrobial peptide PAJE could be a potential candidate peptide for the treatment of S. aureus, SCVs and their biofilm infections, which provided theoretical support for the development of reduce and replace antibiotics products.

    Table and Figures | Reference | Related Articles | Metrics
    Identification of Streptomyces rochei HM85 and Its Biocontrol and Growth-promoting Effects on Sugar Beet
    Zhimin YANG, Huihao ZHANG, Yuanyuan ZHANG, Hongyan DU, Xiaodong LIU, Yaguang HOU, Yi WANG, Daolong XU, Jingui HUANG, Xiaoning CHENG, Yang SUI, Ruili WANG, Chao YU, Lingling ZHAO, Chunmei CHEN, Ru YA, Li JIA, Mingyue ZHANG, Hongwei WANG, Songyao YAO, Ying ZHAO, Ke SHAO
    Journal of Agricultural Science and Technology    2024, 26 (5): 148-155.   DOI: 10.13304/j.nykjdb.2024.0144
    Abstract386)   HTML7)    PDF (2461KB)(40)       Save

    Cercospora leaf sport of beet (CLS), caused by Cercospora beticola, is the most destructive foliar fungal disease of sugar beet worldwide which seriously restricts the development of China’s sugar industry. In order to screen and identify the antagonistic strains with biocontrol applications against Cercospora beticola, the rhizosphere soil samples of sugar beet were collected from Hulunbuir, Inner Mongolia autonomous region and the antagonistic strain HM85 with stable antimicrobial activity was screened by dilution coating flat plate method and dual culture assay. The strain HM85 was identified as Streptomyces rochei based on its morphological,physiological and biochemical characteristics along with 16S rRNA gene sequence analysis.The results of dual culture test showed that the HM85 strain could significantly inhibit the growth of Cercospora beticola HB2-2-2 with inhibitory rate of 58.68%. In addition, the fermentation broth of S. rochei HM85 had antagonistic activity against Cercospora beticola and could promote the growth of sugar beet in pot tests,which plant height, fresh weight of plant,root length,fresh weight of root and root diameter was increased by 22.73%, 20.47%, 36.10%, 32.52%, 75.03% compared to the control, respectively. In summary, S. rochei HM85 could be exploited as a potential bio-inoculant improving production and biocontrol agent for sugar beet leaf sport disease control.

    Table and Figures | Reference | Related Articles | Metrics
    Exploration on Intelligent Path of Animal Husbandry from Perspective of Metauniverse
    Fan YANG, Qingning LIN, Qi WANG, Shiping MAO
    Journal of Agricultural Science and Technology    2024, 26 (8): 1-8.   DOI: 10.13304/j.nykjdb.2023.0406
    Abstract347)   HTML9)    PDF (484KB)(83)       Save

    The metaverse, as a prominent paradigm in the future digital world, presents a viable framework for the transformation and advancement of the livestock industry. Grounded in the metaverse perspective, this paper explored the pathway for the implementation of intelligent livestock farming, and discussed the current state and existing challenges in the intelligentization of the livestock industry. It proposed an intelligent pathway supported by technologies such as blockchain, artificial intelligence (AI) and the internet of things (IoT). The realization pathway for intelligent livestock farming primarily encompassed the following aspects: firstly, implementing intelligent livestock farming through the utilization of big data and IoT technologies, involving systematic data collection and precise analysis to optmize production efficiency and monitor the health status of animals; secondly, adopting blockchain technology to record the origin, production process, and distribution chain of livestock products, thereby establishing a fully traceable product quality assurance system; thirdly, utilizing virtual education platforms for livestock industry training and education, assisting users in acquiring knowledge and skills within a virtual environment, ultimately enhancing technological innovation capabilities; and finally, leveraging the dual roles of government and market to activate elements such as funds, talent and technology, thereby propelling technological innovation and industry transformation and upgrading in the livestock sector. Based on these considerations, it was anticipated that the guidance of digital technology should lead to the high-quality development of the livestock industry.

    Reference | Related Articles | Metrics
    Active Ingredients and Action Mechanism of Agricultural Plant Jiaosu
    Bing LI, Xiumin ZHU, Dai LI, Junxia DU
    Journal of Agricultural Science and Technology    2024, 26 (7): 156-165.   DOI: 10.13304/j.nykjdb.2023.0660
    Abstract344)   HTML2)    PDF (618KB)(750)       Save

    Agricultural plant Jiaosu is a product containing specific bioactive ingredients which is derived primarily from plants through microbial fermentation. It could be applied in agriculture, animal husbandry and soil improvement. Agricultural plant Jiaosu plays significant roles in enhancing crop yields, soil quality and pest resistance, making it a key player in the establishment of green ecological agriculture. In this paper, the classification, characteristics and various effective components of agricultural plant Jiaosu were briefly introduced; the positive effects on crop yield increase, antibacterial properties, soil enhancement and environmental protection of agricultural plant Jiaosu were summarized; the mechanisms were analyzed, the existing issues were identified, and the future development of agricultural plant Jiaosu was discussed.

    Reference | Related Articles | Metrics
    Genetic Diversity Analysis of 255 Maize Germplasm Resources Based on 10K SNP Chip
    Shan GAO, Xiaocui YAN, Nan WANG, Mengjie ZHANG, Youpeng LI, Wenda DIAO, Huijun DUAN
    Journal of Agricultural Science and Technology    2024, 26 (8): 20-33.   DOI: 10.13304/j.nykjdb.2023.0801
    Abstract322)   HTML12)    PDF (2016KB)(148)       Save

    Maize (Zea mays L.) is one of the world’s top three food crops and an important feed crop. Analyzing the agronomic phenotypic traits and genotypic genetic diversity of maize germplasm resources has important guiding significance for crop breeding and production. A natural population composed of 255 germplasm resources was used as the research material, and the genotyping was obtained by whole genome scanning using 10K SNP liquid chip. Phenotypic clustering and genetic diversity analysis were performed using VCF2PCA cluster and Mega11 software. The results showed that 14 139 high quality SNP sites were detected, which were distributed on 10 chromosomes of maize. The polymorphisms ranged from 0.05 to 0.50, with an average of 0.29. Polymorphism information content (PIC) varied from 0.04 to 0.38 with an average of 0.24. 104 SNPs with allele frequency difference were more than 0.30 between tropical and temperate populations. Through population genetic structure analysis, principal component analysis and cluster analysis, 255 samples were mainly divided into temperate and tropical subgroups. These results identified the genetic diversity and relationships among 255 germplasm resources from temperate and tropical maize, laying the foundation for subsequent maize germplasm improvement and innovation.

    Table and Figures | Reference | Related Articles | Metrics
    Research and Application of AIS Data in Marine Fisheries
    Linlin YU, Wei FAN, Heng ZHANG, Yang DAI, Lijun WAN, Fei WANG, Yongchuang SHI, Shenglong YANG
    Journal of Agricultural Science and Technology    2024, 26 (5): 212-222.   DOI: 10.13304/j.nykjdb.2023.0330
    Abstract308)   HTML4)    PDF (563KB)(87)       Save

    Against the backdrop of the global decline of major economic fisheries resources in the global oceans, how to protect and sustainably develop ocean fisheries resources has been highly valued by countries, regions, and organizations worldwide, and has been a hot research topic. Due to limitations of traditional marine fisheries data, it has been difficult to fully understand the catch footprint of distant fishing vessels and make effective monitoring and management. The global distant fishing vessel trajectory data provided by the automatic identification system (AIS) can be used to quantitatively analyze the behavior of individual to global fishing vessels, and the historical catch intensity spatial information uncovered can provide a good alternative data source for monitoring and management of marine fishing activities and assessment of ecological pressure, becoming a new hotspot in marine information and marine fisheries research in recent years. In order to promote the research and application of AIS data in China’s marine fisheries, the research contents and application status of AIS in recent years were summarized and introduced, the shortcomings of AIS data research at present and potential research methods in the future pointed out, which provided a reference for the research and application of AIS in marine fisheries.

    Reference | Related Articles | Metrics
    Cloning and Expression Analysis of Wheat TaCOBL- 5 Genes
    Xinyue BAO, Hongmin CHEN, Weiwei WANG, Yimiao TANG, Zhaofeng FANG, Jinxiu MA, Dezhou WANG, Jinghong ZUO, Zhanjun YAO
    Journal of Agricultural Science and Technology    2024, 26 (6): 11-21.   DOI: 10.13304/j.nykjdb.2024.0172
    Abstract291)   HTML22)    PDF (3595KB)(628)       Save

    Wheat yield directly impacts food security in China, but various abiotic stresses such as drought, low temperature, salt and high temperature severely constrain wheat production. In previous analysis, transcriptomic data revealed differential expression of wheat TaCOBL-5D under multiple abiotic stresses. TaCOBL-5D and its homologous genes TaCOBL-5A and TaCOBL-5B were cloned and obtained, and the comprehensive bioinformatics and expression pattern were analyzed. The results showed that TaCOBL-5 genes exhibited significant conservation in gene structure, protein tertiary structure, conserved domains, and promoter regulatory elements with COBL genes from other species. TaCOBL-5 genes exhibited the highest expression level in roots and responded differently to various abiotic stresses, especially significantly down-regulated under drought stress, indicating its importance under drought stress; it also showed different responses to low temperature, high temperature, and salt stress. In addition, the expression level of the TaCOBL-5D gene varied significantly among different drought-resistant and high-temperature-resistant materials, further suggesting its important role in stress resistance. These findings laid the foundation for further understanding of the function of COBL genes in wheat and provided scientific support for wheat breeding efforts aimed at enhancing stress tolerance.

    Table and Figures | Reference | Related Articles | Metrics
    Bioinformatics and Expression Pattern Analysis of Rice RR Gene Family
    Mingdi CHEN, Guihua HU, Haiwen ZHANG, Wangtian WANG
    Journal of Agricultural Science and Technology    2024, 26 (5): 20-29.   DOI: 10.13304/j.nykjdb.2024.0190
    Abstract288)   HTML4)    PDF (2703KB)(93)       Save

    The rice response regulators (RR) family play important roles in plant growth and development, so it is important to explore the evolution and biological characteristics of the RR genes in rice. There were 22 RR genes in rice, including 15 A-type and 7 B-type genes. Analysis results showed that the rice RR proteins differed in their physicochemical properties, among which the B-type RRs were all hydrophilic proteins (GRAVY<0); homologous sequence analysis revealed that there were 5 paralogous gene pairs in the rice RR genes, namely, OsRR8 and OsRR15OsRR5 and OsRR11OsRR9 and OsRR10OsRR1 and OsRR2, and OsRR20 and OsRR18, which were predicted to have similar functions with known functional genes; the conserved Motif 1 and Motif 3 both belonged to the REC superfamily, which acted as response regulators to receive signals from the two-component phosphotransferase system, and they distributed among 22 OsRR proteins. The analysis of regulatory elements and expression pattern revealed that RR genes had potential ability in the regulating hormone signals transduction such as abscisic acid, salicylic acid, jasmonic acid, gibberellin, and growth hormone, and participated in plant defense against stress such as low temperature, drought, and light and anaerobic signals response, and also involved in the the regulation of different developmental processes of rice etc. functions. The bioinformatics analysis of rice RR gene family provided a theoretical basis for the in-depth study of their functions, and layed the foundation for further revealing the growth, development, and signal transduction mechanisms of plant.

    Table and Figures | Reference | Related Articles | Metrics
    Principles of Microbial Electrochemical Technology and Its Application in the Recycling of Livestock and Poultry Wastes
    Kunhong JIANG, Zhenying XU, Zhenzhen GUO, Lin BAI, Xiaoxia HAO, Dongmei JIANG, Shixiu QIU
    Journal of Agricultural Science and Technology    2024, 26 (7): 210-222.   DOI: 10.13304/j.nykjdb.2022.1053
    Abstract273)   HTML3)    PDF (1655KB)(1608)       Save

    The environment has been under great pressure as a consequence of the livestock waste discharged in the course of intensive production of livestock and poultry farming. By studying the elemental theory of respiratory metabolism, comprised of small molecule acid metabolism of Geobacter sulfurreducens, the aerobic and anaerobic metabolism of Shewanella oneidensis, as well as extracellular electron transfer directly contacted and mediated by electrically conductive pili and electron shuttle substances, the central carbon metabolism pathway, basic extracellular electron transport process and main regulatory mechanisms of model electroactive microorganisms were examined. Owing to insights into the respiration and metabolism of electroactive bacteria, a variety of microbial electrochemical systems (MES) mainly based on microbial fuel cell, microbial electrolytic cell and electric-field-assisted aerobic compost had been developed and optimized. Not only MES were employed to reduce contents of hazardous substances including chemical oxygen demand, greenhouse gases, antibiotics and resistance genes, but they were also used to improve power generation efficiency, cathode high value-added products and humus contents of compost. The research progress of microbial electrochemical theory and thoroughly demonstrates the application of microbial electrochemical technology in the recycling of livestock and poultry waste were reviewed, so as to provide reference and theoretical foundation for the study of livestock waste utilization.

    Table and Figures | Reference | Related Articles | Metrics
    Discovering of QTLs and Candidate Genes Related to Rice Heading Period Traits Based on BSA-seq
    Ronghua WEI, Ming YIN, Wensheng WANG, Yanru CUI
    Journal of Agricultural Science and Technology    2024, 26 (9): 12-24.   DOI: 10.13304/j.nykjdb.2024.0216
    Abstract269)   HTML20)    PDF (6935KB)(98)       Save

    As one of the important traits of rice, rice heading date is closely related not only to the rice growth period but also to rice yield, quality and stress resistance, which determines the planting area and seasonal adaptability of rice varieties. Therefore, the location of rice heading date related genes plays an important role in rice production. In this study, HQ20, Jin 23B and their F4 population constructed were used as the materials. According to the heading stage of the rice in the population, it was divided into 3 levels including early, middle and green plant (not heading), and 3 mixed pools of early, middle and green were constructed. The bulked-segregant analysis sequencing (BSA-seq) method was used to discover rice heading period genes. The results showed that the comparison rate between BSA-seq results and the reference genome was over 94%, and the 4× genome coverage rate was over 80%. Based on this, when the confidence level was 0.95, by SNP-index algorithm 17 SNPs located on 4 chromosomes (6, 7, 8 and 9) were obtained which had consistent association intervals with indel. Further analysis of candidate genes in the region found that 61 genes had non-synonymous mutations. GO and KEGG enrichment analysis showed that pathways closely related to rice heading stage included UDP-glycosyltransferase activity, positive regulation of cell cycle G2/M phase transition, and phytohormone signal transduction. Finally, combining haplotype analysis in the 3K rice database and published expression data, 3 key candidate genes for heading stage were discovered including LOC_Os07g22720LOC_Os07g23740 and LOC_Os08g07200. The above results laid the foundation for genetic improvement of rice heading period traits and analysis of genetic basis.

    Table and Figures | Reference | Related Articles | Metrics
    Research Progress on Effects of Medium and Trace Elements on Yield and Quality of Rice
    Liang SUN, Yi XU, Qin CAI, Jinghao GUO, Can ZHAO, Baowei GUO, Zhipeng XING, Zhongyang HUO, Hongcheng ZHANG, Yajie HU
    Journal of Agricultural Science and Technology    2024, 26 (8): 9-19.   DOI: 10.13304/j.nykjdb.2024.0141
    Abstract267)   HTML25)    PDF (726KB)(192)       Save

    As essential nutrients for rice growth and development, medium and trace elements are the components and activators of enzyme systems, and also participate in a series of important physiological processes, such as photosynthesis, respiration, hormone synthesis and disease defense, which play vital roles in the formation of yield and quality for rice. Therefore, the effects of the absorption, accumulation and metabolic pathways of medium and trace elements, such as magnesium, zinc, seleniumon and iron, on rice yield and quality were reviewed, the challenges faced by the medium and trace elements in rice cultivation were analyzed, and the importance of medium and trace elements and the research direction in sustainable agriculture in the future were discussed, which provided theoretical basis for achieving high yield and high quality of rice.

    Table and Figures | Reference | Related Articles | Metrics
    Research Status and Development Trend of Rapid Detection of Agglutinin in Concanavalin A
    Xiaoxiao ZHANG, Xiaoqian LI, Cheng ZHU, Chenze LYU
    Journal of Agricultural Science and Technology    2024, 26 (6): 214-225.   DOI: 10.13304/j.nykjdb.2023.0098
    Abstract260)   HTML1)    PDF (1938KB)(61)       Save

    Concanavalin A (Con A) is mainly found in jack bean, and it is a plant lectin that can specifically bind to polysaccharides, glycoproteins and other substances. Con A has anti-nutritional and anti-pest effects, which is an important part of plant self-protection, and also the main cause of food poisoning and allergic reactions caused by eating legumes. Therefore, the detection of Con A is of great significance for legume vegetable breeding and food safety assessment. The research progress of novel rapid detection technologies for Con A was introduced in detail, including nanomaterial based biosensor, immune sensing technology, sugar sensing technology and aptamer sensing technology. Biosensors based on nanomaterials had high accuracy but lack biological specificity; the immune sensing technology had improved the detection speed of the traditional immune method but still had the problem of insufficient signal repeatability; sugar sensing technology had greatly improved the specificity and detection accuracy of hemagglutination method, but it could not distinguish lectins with the same sugar specificity. Aptamers based detection technology had excellent detection accuracy, specificity and repeatability, and unnatural aptamers could be obtained through chemical modification to further enhance the specificity, which should be the development trend of rapid detection of Con A in the future.

    Table and Figures | Reference | Related Articles | Metrics
    Application of Improved YOLOv5 Model in Citrus Recognition in Natural Environment
    Jun TIE, Jie ZHAO, Lu ZHENG, Lifeng WU, Bowen HONG
    Journal of Agricultural Science and Technology    2024, 26 (7): 111-120.   DOI: 10.13304/j.nykjdb.2022.0994
    Abstract252)   HTML13)    PDF (5892KB)(494)       Save

    Green citrus in complex natural environment had different growth forms and similar color to the background color, so a detection method based on hybrid attention mechanism and improved YOLOv5 model was proposed to effectively identify green citrus. Firstly, the method improved the network structure of YOLOv5 by adding a hybrid attention mechanism in the backbone network etc., embedding SE (squeeze and excitation) attention in layer 2 and CA (coordinate attention) attention in layer 11 of the backbone network; secondly, it improved the feature fusion structure of the network model, the lower branch was placed before the model C3 module, by combining the YOLOv5 model and concat feature fusion operation,and then the features were fused with another upper branch; finally, the classification loss function of the model was improved, and the classification loss function of the YOLOv5 model was changed to Varifocal Loss function to enhance the extraction of green citrus feature information and improve the accuracy of green citrus detection. According to the natural environment and the characteristics of the citrus itself, the self-built dataset was classified and 3 sets of comparison tests of citrus under different classification scenarios were designed to verify its effectiveness. The test results showed that the improved YOLOv5-SC model had higher precision and better robustness for the recognition of green citrus in natural environment, which accuracy was 91.74%, average accuracy was 95.09%, and F1 was 89.56%, and it provided technical support for smart picking of green fruits.

    Table and Figures | Reference | Related Articles | Metrics