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Research Progress on Biocontrol Mechanism and Synergistic Disease Prevention of Trichoderma Xiao WEI, Chunxia CAO, Daye HUANG, Jingwu YAO, Qinfeng YUAN Journal of Agricultural Science and Technology    2024, 26 (11): 126-135.   DOI: 10.13304/j.nykjdb.2023.0524 Abstract （1402）   HTML （5）    PDF （551KB）（78）       Knowledge map    Save Trichoderma is a widely distributed filamentous fungus and widely used as a biological fungicide to control a variety of plant diseases in agriculture due to its excellent biocontrol potential. Trichoderma can reduce plant diseases caused by pathogens through different direct mechanisms （mycoparasitism and the production of cell wall degrading enzymes， antibiosis， competition for space or nutrients） or indirect mechanisms （induction of plant defense ）. Compound biocontrol agents have received increasing attention in recent years. Trichoderma can be used in combination with other biocontrol products to produce better biocontrol effects because of its resistance to chemical pesticides and compatibility with rhizosphere microorganisms. The related mechanisms of Trichoderma， including parasitism， antibiosis， nutrient competition and induction of plant resistance were summarized， and the current research progress were discussed. In addition， the research progress of synergistic disease prevention between Trichoderma and other microorganisms or non-biological （chemical fungicides， other substances） with disease prevention effect was reviewed， and the current research status and the subsequent development prospects of Trichoderma as a biological fungicide were explored. Table and Figures | Reference | Related Articles | Metrics

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Identification and Biological Characteristics of a Colletotrichum fructicola Causing Chestnut Internal Rot Disease Shifeng MU, Xiaolei WEN, Lina FENG, Dexuan ZHAO, Suhong GAO, Peng GAO, Huixia QI Journal of Agricultural Science and Technology    2024, 26 (9): 122-128.   DOI: 10.13304/j.nykjdb.2023.0871 Abstract （1367）   HTML （4）    PDF （1614KB）（43）       Knowledge map    Save Chestnut internal rot is the main disease of chestnut during postharvest storage， which causes rot and mildew of chestnut kernel and brings huge economic losses to production. In order to clarify the pathogenic bacteria and its biological characteristics， the strain ZHZF21 was obtained by tissue isolation method. The strain was identified by morphological characteristics and ITS， TUB2 and CAL multi-gene sequence analysis. The pathogenicity of strain ZHZF21 was determined by mycelial inoculation method， and its biological characteristics were studied. The results showed that the colony of strain ZHZF21 was dark green ring concentric circles， with sexual and asexual reproduction stages. This strain was clustered with Colletotrichum fructicola numbered NDSTY31 in GenBank based on multi-gene sequences. Combined with colony morphological characteristics and molecular tree， strain ZHZF21 was preliminarily identified as Colletotrichum fructicola. The brown lesions after inoculation of strain ZHZF21 were consistent with the natural symptoms. The strain ZHZF21 could grow at 5~35 ℃， the optimum temperature was 25 ℃， the lethal temperature was 45~50 ℃， and the optimum pH was 6. Under the condition of full darkness， strain ZHZF21 grew fastest， the utilization rates of arabinose and yeast were the highest， the utilization rate of lactose was the lowest， and urea could hardly be used. Table and Figures | Reference | Related Articles | Metrics

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Progress of Research on Alleviating Obstacles of Continuous Cropping by Soil Sterilization and Arbuscular Mycorrhizal Fungi Yaping FAN, Baiquan SONG, Changxian WANG Journal of Agricultural Science and Technology    2024, 26 (10): 158-167.   DOI: 10.13304/j.nykjdb.2023.0386 Abstract （1362）   HTML （4）    PDF （562KB）（59）       Knowledge map    Save The continuous cropping obstacles have been widespread in horticultural production， and the reasons were complicated. It has done serious harm to the ecosystem consisting of soil， plants and rhizosphere microorganisms. The economical and convenient soil sterilization technologies effectively alleviate the obstacles， among the sterilization technologies， high temperature sterilization and reductive soil disinfestation improve soil environment. Meanwhile， symbiotic associations between arbuscular mycorrhizal （AM） fungi and plant root systems is helpful to reconstruction of rhizosphere ecosystems. Focused on the continuous cropping obstacles， this paper reviewed the effect of high temperature sterilization， reductive soil disinfestation and arbuscular mycorrhizal fungi on the obstacles alleviation and their mechanisms. Furthermore， meaningful research works in this field were prospected. The review provided a theoretical and technical basis for the alleviation of continuous cropping obstacles in horticultural production. Table and Figures | Reference | Related Articles | Metrics

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Effect of Different Storage Temperatures on Quality of Jingcai 1 Watermelon Lanting XIANG, Shuhui SONG, Lijuan LIU, Xiaoling SHE, Jiahua ZHOU, Baogang WANG, Hong CHANG, Chao ZHANG, Daqi FU, Yunxiang WANG Journal of Agricultural Science and Technology    2024, 26 (9): 137-145.   DOI: 10.13304/j.nykjdb.2023.0685 Abstract （1317）   HTML （0）    PDF （1639KB）（29）       Knowledge map    Save To explore the effect of storage temperature on Jingcai 1 watermelon， the fruits of Jingcai 1 watermelon were storaged under 5， 15 and 25 ℃. The firmness， rate of weight loss， chilling injury， soluble solids， pectin， cellulose， soluble sugar， organic acid， lycopene， β?-carotene and vitamin C contents were determined under different temperature treatments. The results showed that， with the storage extending， the weight loss rate of Jingcai 1 watermelon fruit increased， the fruit firmness decreased， the content of water-soluble pectin （WSP） increased， the sodium carbonate-soluble pectin （SSP） decreased， the vitamin C， lycopene and β?-carotene showed decreasing trend， the variation of soluble solids fluctuated within a small rage， the cellulose decreased at first and then increased， while the content of soluble sugar and organic acid showed different changing trends at different storage temperatures. When stored at 5 ℃， the vitamin C and soluble sugar of fruit could be high level， but a long-term storage would raise the chilling injury rate and cause gradual wastage of nutrients， resulting undoubtedly in loss of the edible and commercial values. When stored at 15 ℃， the contents of water， organic acids and cytochromes could be better maintained， giving watermelons better quality and flavor. When stored at 25 ℃， the quick loss of soluble sugar， organic acid and pectin of the fruit leaded to soft rot， depriving original flavors of watermelons and making it difficult to maintain its quality. Above results provided a theoretical basis for the commercialization of its storage， transportation and preservation. Table and Figures | Reference | Related Articles | Metrics

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Study on Insecticidal Activity Constituents and Mechanism of Artemisia annua Against Mythimna separate Chunyan LI, Qian LIANG, Chengxiang GAO, Zongsuo LIANG, Fang WANGA Journal of Agricultural Science and Technology    2024, 26 (12): 129-137.   DOI: 10.13304/j.nykjdb.2023.0589 Abstract （1268）   HTML （2）    PDF （1636KB）（214）       Knowledge map    Save In order to study the insecticidal activity components and preliminary mechanism of the petroleum ether extract from Artemisia annua against Mythimna separata， the insecticidal activity of different fractions and main components were measured by insect immersion method. The petroleum ether extract was separated by column chromatography and analyzed by gas chromatography-mass spectrometry to determine its chemical components， and the target enzyme and metabolic enzyme activities in the body of Mythimna separata were measured for the active components. The results showed that the petroleum ether extract of Artemisia annua was separated by silica gel column chromatography， and 27 fractions were obtained， of which fraction 8 showed the best contact toxicity. According to gas chromatography-mass spectrometry， the main compounds were tridecane， 4-quinolinecarboxaldehyde， caryophyllene oxide， linoleic acid ethyl ester， linolenic acid ethyl ester with contents of 7.9%， 72.8%， 3.6%， 2.4%， 3.1%，respectively. The lethal concentration of 50% （LC50） of above 5 compounds on the 7th day were 7.9， 2.5， 3.6， 5.4 and 1.5 mg·mL-1， respectively， which indicated that ethyl linolenic acid had significant contact toxicity against M. separata. On the 7th day of treatment with 1.5 mg·mL-1 linolenic acid ethyl ester， the scanning electron microscopy results showed that the wax layer structure in the surface of M. separata were loosely， the wax layer particles disappeared significantly， and the wax flower gap increased. And the acetylcholinesterase （AChE） and carboxylesterase （CarE） of M. separata treated with 1.5 mg·mL-1 linolenic acid ethyl ester showed first activation and then inhibition， Na+-K+-ATPase showed inhibition over time， and catalase （CAT） showed first inhibition and then activation. To sum up， the linolenic acid ethyl ester was the main active compound of A. annua， which provided reference for the development of new plant-based insecticides and biological control of M. separata and the development and utilization of A.annua resources. Table and Figures | Reference | Related Articles | Metrics

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Evaluation of the Growth-promoting Effect of Trichoderma harzianum on Tobacco and Its Induced Resistance to Black Shank Disease Junjia CHANG, Jiaxin GAI, Gang TAO, Zhuanlonghai MO Journal of Agricultural Science and Technology    2024, 26 (10): 168-176.   DOI: 10.13304/j.nykjdb.2024.0225 Abstract （1237）   HTML （3）    PDF （2248KB）（49）       Knowledge map    Save To investigate the growth-promoting effects of Trichoderma harzianum on tobacco and its induced resistance mechanisms against tobacco black shank disease caused by Phytophthora nicotianae， 5 Trichoderma strains were screened. The results showed that the strain T. harzianum ACCC 32808 （TH32808） was demonstrated the strongest inhibition （62.00%） among 5 strains of Trichoderma. The growth-promoting effects of TH32808 on different tobacco varieties and its induced resistance against tobacco black shank disease were assessed. The results showed that， under pot condition， TH32808 exhibited different ability on promoting the growth of different tobacco varieties. Under TH32808 treatment， the fresh weight of Honghuadajinyuan increased by 8.30%， the control efficacy against black shank disease in Honghuadajinyuan was 51.74%. In Honghuadajinyuan and K326， the catalase activity peaked on the 4th day， reaching 1.42 and 2.15 times that of CK， the peroxidase activity peaked on the 2nd day， being 2.49 and 2.29 times that of CK， the superoxide dismutase activity peaked on the 4th day， reaching 1.17 and 1.15 times that of CK， respectively. Moreover， TH32808 could induce resistance against black shank disease， key genes involved in the metabolic pathways of salicylic acid， jasmonic acid and ethylene， such as NPR1， PDF1.2， EFE and NtSGT1， all exhibited an upregulation trend. Above results provided fungal resources and theoretical basis for the biological control and induced resistance research against black shank disease in tobacco. Table and Figures | Reference | Related Articles | Metrics

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Effect of Exogenous NO on Expression of Tomato Antioxidant Enzyme Gene Under Tomato Yellowing Leaf Curl Virus Stress Jincheng LUO, Xiaolin ZHU, Xiaohong WEI, Xian WANG, Baoqiang WANG, Xuefen DU Journal of Agricultural Science and Technology    2025, 27 (2): 125-135.   DOI: 10.13304/j.nykjdb.2023.0647 Abstract （1214）   HTML （2）    PDF （2999KB）（96）       Knowledge map    Save In order to investigate the effect of exogenous nitric oxide （NO） on the expression of tomato antioxidant gene under the stress of tomato yellow leaf curl virus （TYLCV）， the susceptible tomato Jinpeng 1 was used as the experimental material. Transcriptome sequencing， fluorescent qRT-PCR and bioinformatics analysis were performed under control （CK）， TYLCV （TY） and NO+TYLCV （NO+TY） treatments. The results showed that a total of 55 antioxidase-coding genes were selected in the tomato genome， among which the antioxidase-containing genes with the highest number of exons in different subcellular regions were significantly responsive to TYLCV stress. The number of NO-mediated antioxidant enzyme coding genes in different subcellular compartms showed chloroplast>cell membrane>cytoplasm>peroxisome>vacuole， in which the expression levels of Chl Cu-Zn SOD， Chl MR2， Chl GR， Per MR， Pla CAT1 and Pla CAT7 were significantly up-regulated， and the expression levels of Chl Fe SOD1， Chl Fe SOD2， Cyt GPX， Cyt APX1， Cyt APX2 L-5， Pla CAT3， Pla CAT8 and Vac CAT were significantly down-regulated. Fluorescence qRT-PCR verified that Chl GR， Min Mn SOD and Per CAT2 responded to TYLCV， and Chl Cu-Zn SOD， Pla CAT7， Pla CAT8 and Cyt APX2 L-5 responded to TYLCV and NO. Above results provided theoretical basis for studying the mechanism of NO in improving tomato disease resistance. Table and Figures | Reference | Related Articles | Metrics

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Application of UV-visible Spectral Library in Recessive Screening of Pesticide Fungicides Ying CHEN, Xiaofeng LIU, Yiping CHEN, Liping LIAO, Hongtao FU Journal of Agricultural Science and Technology    2024, 26 (12): 122-128.   DOI: 10.13304/j.nykjdb.2023.0247 Abstract （1213）   HTML （1）    PDF （1444KB）（25）       Knowledge map    Save To establish the ultraviolet-visible （UV-VIS） spectral library to quickly and accurately retrieve the hidden components added in the pesticide fungicide， with acetonitrile and water as the mobile phase， the UV-VIS spectrograms of 98 conventional bactericide standards were separated and extracted by gradient elution using a high-performance liquid chromatograph， and the UV-VIS spectrograms of the standard substances were used to establish the UV-VIS spectrogram library. The target compound was matched according to the spectral similarity and the retention time of the standard sample， so as to retrieve the hidden components added in the pesticide preparation. 2 bactericides were used to verify the accuracy of the spectral library retrieval， and the results showed that the accuracy of the spectral library retrieval reached 100%. This method had the advantages of high accuracy， easy operation and low cost. Table and Figures | Reference | Related Articles | Metrics

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Fruit Quality Analysis of Prunus domestica × armeniaca at Different Harvest Maturity Levels Ruyue WANG, Haifang HU, Shasha LUO, Ziyi ZHEN, Yeyong XU, Xiaojing HU Journal of Agricultural Science and Technology    2025, 27 (2): 158-169.   DOI: 10.13304/j.nykjdb.2023.0429 Abstract （1207）   HTML （5）    PDF （3014KB）（46）       Knowledge map    Save To study the effects of different harvest maturity levels on the quality of P. domestica × armeniaca fruits， and to screen out the suitable maturity levels for picking P. domestica × armeniaca，using P. domestica × armeniaca ‘Fengweihuanghou’ as the test materials， the changes in indicators such as single fruit weight， fruit color and fruit hardness and so on under different maturity levels（Ⅰ， Ⅱ， Ⅲ， Ⅳ， Ⅴ） were analyzed by variance， correlation and principal component analysis. The results showed that with the increase of harvest maturity levels， the red green value （a*） of the fruit skin， the red green value （a*） of the fruit flesh， the blue yellow value （b*） of the skin， the blue yellow value （b*） of the flesh， the comprehensive ratio of the skin （a*/b*） and the comprehensive ratio of the flesh （a*/b*） all showed a significant upward trend， which increased by 15.811， 15.760， 23.009， 15.380， 0.973 and 0.558， respectively， during the entire maturity and development period， and were highly significant positive correlation with fruit maturity. Chlorophyll a+b， peel brightness value （L*）and flesh brightness value （L*） showed significant downward trend， with decrease of 17.230 μg·cm-3， 11.460 and 13.492 during maturity， respectively. The single fruit weight， soluble solids， solid to acid ratio and membrane permeability all showed a significant upward trend with the increase of harvest maturity level. At maturity Ⅴ， the various indicators were 101.500 g， 18.780%， 85.535% and 62.050%， respectively， and showed a highly significant correlation with maturity level. The skin hardness， peel hardness and titratable acid showed a significant downward trend with the delay of maturity， reaching their lowest values at maturity Ⅴ， which were 13.770 N， 6.886 N and 0.246%， respectively， which were significantly negatively correlated with harvest maturity level. The principal component analysis showed that the fruit quality of different maturity level was as follows： maturity Ⅴ>maturity Ⅳ>maturity Ⅲ>maturity Ⅱ>maturity Ⅰ. Based on production needs and comprehensive economic benefits， maturity Ⅰ and Ⅱ were not suitable for harvesting，and maturity Ⅳ and Ⅴ had good quality and taste， but the fruit softens severely. Maturity Ⅲ could be selected as the appropriate harvesting maturity. Above results could provide theoretical basis and scientific basis for the subsequent research and production of P. domestica × armeniaca . Table and Figures | Reference | Related Articles | Metrics

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Study on Control Effect of Streptomyces phaeoluteichromatogenes HEBRC45958 Strain on Corynespora Leaf Spot of Tomato Daye HUANG, Zhibin YU, Zhongyi WAN, Dan YANG, Jinping LI, Chunxia CAO Journal of Agricultural Science and Technology    2024, 26 (11): 136-142.   DOI: 10.13304/j.nykjdb.2023.0416 Abstract （1198）   HTML （2）    PDF （3075KB）（27）       Knowledge map    Save In order to clarify the control effect of Streptomyces phaeoluteichromatogenes strain HEBRC45958 against Corynespora leaf spot of tomato， the antifungal activity was determined in vitro by using dual culture assay combined with microscope and scanning electron microscope observation. The siderophore production and the activities of hydrolases were also determined. The control effect of the fermentation broth of HEBRC45958 strain on Corynespora leaf spot of tomato was determined in pots. The results showed that the inhibition rate HEBRC45958 on Corynespora cassiicola was 52.65% in the dual culture. Compared with control， the mycelium treated with HEBRC45958 strain was distorted and cracked by scanning microscopy， and the space between the cell wall and cell membrane was widened by transmission electron microscopy. The HEBRC45958 strain could produce amylase， cellulase， chitinase and siderophore. The control effect of fermentation broth against Corynespora leaf spot of tomato was 100% in pot experiment， which showed that HEBRC45958 strain had a good prospect of biocontrol application for Corynespora leaf spot of tomato. Table and Figures | Reference | Related Articles | Metrics

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Effects of Bacillus thuringiensi on Intestinal Bacteria in Ectropis obliqua Ruoheng JIN, Xiaoyu LI, Jingwu YAO, Beibei WANG, Chunxia CAO, Daye HUANG Journal of Agricultural Science and Technology    2025, 27 (2): 141-149.   DOI: 10.13304/j.nykjdb.2023.0794 Abstract （1150）   HTML （1）    PDF （3110KB）（25）       Knowledge map    Save Ectropis obliqua is one of the most important pests on tea， which has brought serious economic losses to the tea industry. Bacillus thuringiensis （Bt）?， as a key biological insecticide for the control of E. obliqua， plays an important role in the green prevention and control of diseases and pests in tea gardens. To clarify the effect of Bt treatment on intestinal bacteria of E. obliqua larvae， the Illumina platform using 16S rDNA high-throughput sequencing was applied to compare the structure and population diversity of intestinal commensal community in E. obliqua larvae treated with different dosage of Bt suspensions. The results showed that， compared with CK treatment， the intestinal bacterial composition of E. obliqua larvae treated with lower dosage of Bt did not change significantly， but those treated with higher dosage of Bt had significant effect on the composition of intestinal bacteria in E. obliqua larvae. And the treatments with higher dosage of Bt significantly increased the diversity and abundance of intestinal bacteria in E. obliqua larvae， which indicated that intestinal microorganisms were involved in the response of E. obliqua larvae to Bt infection. Above results provided basis for further exploring the insecticidal mechanism of Bt， and provided new ideas for improving the virulence of Bt. Table and Figures | Reference | Related Articles | Metrics

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Effect of Glycosylation on Quality and Volatile Flavor Characteristics of rainbow trout （ Oncorhynchus mykiss） Floss Lei JI, Tianhong LIU, Ying WANG, Xiao LI, Hongyan LI, Xiaodong JIANG, Yuanqin SUN, Shuaizhong ZHANG Journal of Agricultural Science and Technology    2024, 26 (9): 159-172.   DOI: 10.13304/j.nykjdb.2023.0731 Abstract （1066）   HTML （0）    PDF （1304KB）（19）       Knowledge map    Save To promote the development of high-value products for rainbow trout （Oncorhynchus mykiss）， the quality （physicochemical properties， color， and sensory properties） of rainbow trout floss with different sugars added were investigated. At the same time， the volatile components and fatty acids of the floss were analyzed by gas chromatography-mass spectrometer （GC-MS）. The results showed that the addition of sugar had an important impact on the quality of rainbow trout floss， which could promote the process of Maillard reaction and improve the sensory quality of fish floss such as taste and color. As pentose， the fish floss with arabinose had a faster process of Maillard reaction and more obvious color change， while the fish floss with fructooligosaccharides had a better sweet taste. In terms of fatty acid， the contents of saturated fat acid， eicosapntemacnioc acid （EPA） and docosahexaenoic acid （DHA） of the fish floss with oligofructose were the highest， and the content of polyunsaturated fatty acid of the fish floss with arabinose was the highest. In conclustion， due to Maillard reaction， the fish floss added with sugar produced aldehydes， ketones， alcohols and other substances with lower threshold， which could impact the flavor. Above results provided reference for improving the quality of rainbow trout floss and increasing product added value. Table and Figures | Reference | Related Articles | Metrics

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Symptoms and Pathogen Identification of Ficus carica Bacterial Leaf Spot Disease in Fujian Mengting JI, Ling ZHU, Xiaohua LUO, Yuting ZHENG, Shun XIAO, Fangping HU, Xueqing CAI Journal of Agricultural Science and Technology    2025, 27 (1): 147-154.   DOI: 10.13304/j.nykjdb.2023.0546 Abstract （1058）   HTML （0）    PDF （2145KB）（466）       Knowledge map    Save To clarify the taxonomy of the pathogen causing a new bacterial leaf spot disease on fig （Ficus carica） in Fuzhou in 2020， diseased fig leaves were collected as test materials. The bacterial strains were isolated from the diseased leaves by streak plate method， and then the pathogenicity of the strains was determined by tobacco allergic reaction， spray inoculated fig leaves and Koch’s rule. The pathogen was identified based on morphological characteristics， physiological and biochemical determination， and phylogenetic tree analysis using partial sequences of 16S rDNA， gyrB and rpoD. The results showed that 10 bacterial strains were isolated and purified from the diseased leaves， healthy fig leaves demonstrated similar symptoms as the specimen collected from fields， and the identical morphological bacterial strains were re-isolated from diseased fig leaves. Thus， all of the bacterial strains were identified as the causal pathogens of bacterial leaf spot disease by Koch’s rule. The colony morphology was light yellow， round， and flat on the NA medium after 2 d of culture， and the gram staining reaction was negative. The bacterial strains could produce green water-soluble fluorescent on KB medium. The sequencing analysis based on 16S rDNA， gyrB and rpoD showed that the test strains were clustered with Pseudomonas cichorii； and the results of physiological and biochemical characteristics， Biolog phenotypes instrument， and specific primers were similar to the P. cichorii strain 5707， therefore the 10 isolated bacterial strains were identified as P. cichorii. It was the first report that P. cichorii could naturally cause the leaf spot disease in F. carica. Table and Figures | Reference | Related Articles | Metrics

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Effects of Astragalus Polysaccharides on Production Performance， Carcass Performance and Meat Quality of Stellar-fed Tan Sheep Guoshun CHEN, Bin TIAN, Yancheng GAO, Yancui LI, Wenjin TIAN, Wenjing YANG, Wancheng WU, Lijun HUANG Journal of Agricultural Science and Technology    2025, 27 (2): 150-157.   DOI: 10.13304/j.nykjdb.2023.0541 Abstract （1037）   HTML （3）    PDF （497KB）（29）       Knowledge map    Save To elucidate the effects of astragalus polysaccharides on the growth， slaughtering performance and meat quality of Tan sheep， 24 healthy， similar-weight （22.17±1.21 kg） 50-days-old Tan lambs were randomly divided into 4 groups for fattening trials， including basal diet group （control group）， basal diet+0.05% astragalus polysaccharide group （test group Ⅰ）， basal diet+0.10% astragalus polysaccharide group （test group Ⅱ） and basal diet+0.15% astragalus polysaccharide group （test group Ⅲ）. The weights were determied on 0， 10 and 70 d of the trial， and the slaughter performance and meat quality were analyzed on at the end of the trial （70 d）， respectively. The results showed that， compared with control group， the final body weight （FBW） and average daily gain （ADG） of test group Ⅱ were significantly increased， and the feed conversion rate was significantly decreased. The carcass weight and slaughter rate of test group Ⅱ increased significantly， the cooked meat rate of test group Ⅱ and test group Ⅲ increased significantly. The back fat thickness of test group Ⅰ and test group Ⅱ treatment was significantly reduced， and the L* value of test group Ⅱ was significantly reduced. The contents of glycine， aspartic acid and total amino acids in muscle of Tan sheep treated with test group Ⅱ and test group Ⅲ were significantly increased， and the content of umami amino acids was significantly increased in treatments with astragalus polysaccharides. The contents of monounsaturated fatty acids （MUFA） and polyunsaturated fatty acids in mutton of Tan sheep treated with test group Ⅱ were significantly increased. And the value of MUFA/TFA （total fatty acids） of test group Ⅱ and test group Ⅲ increased significantly. In summary， the addition of 0.10% astragalus polysaccharides to the diet could improve the growth performance， carcass performance and meat quality of Tan sheep. Table and Figures | Reference | Related Articles | Metrics

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Preliminary Function Analysis of GhERF020 Gene in Response to Verticillium Wilt in Cotton Huiting WENG, Haiyang LIU, Huiming GUO, Hongmei CHENG, Jun LI, Chao ZHANG, Xiaofeng SU Journal of Agricultural Science and Technology    2024, 26 (9): 112-121.   DOI: 10.13304/j.nykjdb.2023.0407 Abstract （1028）   HTML （3）    PDF （3709KB）（31）       Knowledge map    Save Verticillium wilt （VW） is a disruptive symptom caused by Verticillium dahliae， which affects fiber yield and quality of cotton in China. AP2/ERF transcription factors play prominent role in plant growth， development and stress response. In order to excavate the genes related to resistance to VW in cotton and explore their biological functions， the candidate gene GhERF020 （XM_016842745.1） was obtained by homologous alignment of AT1G22810.1 related gene of Arabidopsis thaliana against V. dahliae. Bioinformatic analysis showed that the open reading frame length of GhERF020 was 441 bp， encoded 147 amino acids. GhERF020 contained a conserved AP2 domain， the relative molecular weight was 16.16 kD without transmembrane structure， belonging to the hydrophilic protein. It was predicted that the promoter region contained response elements of salicylic acid （SA）， methyl jasmonate （MEJA）， abscisic acid （ABA） and other response elements. The expression of GhERF020 was induced by V. dahliae infection. Subcellular localization analysis showed that GhERF020 protein was localized in the nucleus. As the expression of GhERF020 was downregulated by virus-induced gene silencing （VIGS） technique， the susceptibility of cotton plants to V. dahliae was significantly enhanced， and the disease index and pathogen biomass were distinctly increased. Above results indicated that GhERF020 was a positive regulator in resistance against V. dahliae in cotton. Table and Figures | Reference | Related Articles | Metrics

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Physiological Response of Tobacco Infected by Phytophthora to Trichoderma harzianum Inoculation Sequence Zhichao REN, Yaohui MU, Xuyang YAO, Shue LI, Yongfeng ZHANG, Tianbao REN, Guoshun LIU, Quanyu YIN Journal of Agricultural Science and Technology    2025, 27 (1): 155-164.   DOI: 10.13304/j.nykjdb.2023.0544 Abstract （981）   HTML （0）    PDF （1598KB）（293）       Knowledge map    Save To explore the physiological response of tobacco plant infected by Phytophthora to Trichoderma harzianum application sequence， the pot experiments were carried out with different treatments including inoculated with Phytophthora spore suspension （T1）， inoculated with T. harzianum spore suspension （T2）， inoculated with Phytophthora spore suspension first and then inoculated with T. harzianum spore suspension （T3）， inoculated with T.harzianum spore suspension first and then inoculated with Phytophthora spore suspension （T4）， inoculated with Phytophthora spore suspension and T. harzianum spore suspension at the same time （T5）， and clear water （CK） as controls. The effects of T. harzianum application sequence on biological characteristics， physiological characteristics and induced resistance of tobacco infected by Phytophthora were analyzed. The results showed that， in the growth of tobacco plants， the agronomic characters of tobacco plants with T4 treatment increased the most， and the dry and fresh weight of underground parts increased by 103.73% and 8.30% compared with CK. In the physiological characteristics of tobacco plants， T. harzianum could reduce the inhibition of Phytophthora on photosynthetic pigment accumulation and root activity of tobacco plants， and significantly increase both of them， and it could also strengthen the activities of enzymes related to carbon and nitrogen metabolism in tobacco plants. After transplanting 21 d， the activities of nitrate reductase and glutamine synthetase with T4 treatment increased by 13.14% and 6.40%， respectively. In the control effect， T4 treatment had the lowest incidence and disease index， which were 39.39% and 13.89%， and the highest antioxidant enzyme activity. In conclusion， there were significant differences in the physiological response of tobacco plants to the inoculation sequence of T. harzianum. In production， T. harzianum should be inoculated in advance to promote the growth and development of tobacco and inhibit the incidence of black shank disease. Above results laid a theoretical foundation for the regulation of tobacco growth and development and the comprehensive prevention and control of black shank. Table and Figures | Reference | Related Articles | Metrics

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Comparative Analysis of Honey from Different Sources Based on Multidimensional Indicators Ming ZHANG, Miaomiao CHE, Xinli HUO, Xue WANG, Xing ZHENG, Leilei ZHAO, Yazhou ZHAO Journal of Agricultural Science and Technology    2025, 27 (2): 170-179.   DOI: 10.13304/j.nykjdb.2023.0522 Abstract （967）   HTML （3）    PDF （4114KB）（24）       Knowledge map    Save Honey is a natural product with complex composition and characteristics， which vary depending on the botanical source， origin， bee species and so on. In order to evaluate， differentiate and identify honey samples， a comparative analysis of honey from different sources using multidimensional indicators， including conventional physicochemical analysis and high performance liquid chromatography-quadrupole-time of flight/mass spectrometer （HPLC-Q-TOF/MS） technique were conducted. The results showed significant differences in amylase activity， fructose， glucose， phenolic acid and flavonoid contents among different honey samples. Among them， rape honey exhibited the lowest amylase activity with a value of （3.42±0.70） mL·g-1·h-1， while acacia honey （Qinshui） had the highest amylase activity with a value of （12.66±2.44） mL·g-1·h-1. The total phenolic acid and total flavonoid contents of Apis cerana honey were higher than other honey samples. Pro-His-Cys and hydroxytyrosol 1-O-glucoside might be useful in distinguishing honey from nectar plant species. Palustrine played an important role in differentiating acacia honey from different regions. Above results provided methodological guidance for the characterization and authenticity assessment of honey， that was， the multidimensional index method could be used as a valuable tool for comparative analysis of honey samples from different sources. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on Crop Diseases Identification Based on Convolutional Neural Network Zili CHEN, Wei LIN, Jia HE, Laigang WANG, Guoqing ZHENG, Yilong PENG, Jiadong JIAO, Yan GUO Journal of Agricultural Science and Technology    2025, 27 (4): 99-109.   DOI: 10.13304/j.nykjdb.2023.0785 Abstract （852）   HTML （7）    PDF （1006KB）（116）       Knowledge map    Save Crop diseases are major threats for agricultural production， so timely and accurate identification of disease is important for the development of control measures to ensure food security. With the rapid development of deep learning， convolutional neural networks are used more and more to identify crop diseases. This paper compared the advantages and disadvantages of convolutional neural network disease recognition methods from 3 aspects including disease recognition based on different data sets， disease recognition using transfer learning and pre-training， and lightweight of the disease recognition model. It also analyzed the shortcomings of the current methods and put forward the future development trend. It was pointed out that more abundant data sets should be constructed， multi-modal data should be combined， models should be further optimized， and robots should be used to implement automatic detection. It provided important references for reducing food loss， realizing precision agriculture management， promoting agricultural modernization and sustainable development. Table and Figures | Reference | Related Articles | Metrics

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Effect of Chinese Chestnut Yellow Crinkle Disease on Quality of Chestnut Dexuan ZHO, Peng GAO, Xiaolei WEN, Shifeng MU, Suhong GAO, Lina FENG, Weiming SUN, Huixia QI Journal of Agricultural Science and Technology    2025, 27 (2): 136-140.   DOI: 10.13304/j.nykjdb.2023.0844 Abstract （824）   HTML （1）    PDF （559KB）（14）       Knowledge map    Save In order to comprehensively evaluate the effect of Chinese chestnut yellow crinkle disease on the quality of chestnut nuts， the related indexes of nut quality of trees were determined， and the effect of the disease on nut quality was analyzed. The results showed that， compared with healthy trees， the contents of trace elements in nut of diseased trees were increased， and the contents of amino acids， total phenols and flavonoids were significantly increased by 24.93%， 20.54% and 36.33%， respectively. The chestnut bracts of diseased trees had long and sof spines， uneven color of nuts， the wrinkled surface and the rough texture. The number of grains per bract， the weight of single bract， the full nut rate and the yield per plant of diseased trees were significantly reduced， among them， the yield per plant was reduced by 96.45% compared with the healthy tree. Above results provided a theoretical basis for scientific evaluation of the effect of Chinese chestnut yellow crinkle disease on nut quality. Table and Figures | Reference | Related Articles | Metrics

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Flavor Differences of Different Types of Fresh Corn Based on Electronic Nose and GC-IMS Technology Yaxing SHI, Hui DONG, Li XU, Yanli FAN, Hui LIU, Yamin SHI, Ainian YU, Ning GAO, Jiuran ZHAO, Baishan LU, Ronghuan WANG Journal of Agricultural Science and Technology    2024, 26 (11): 143-156.   DOI: 10.13304/j.nykjdb.2024.0708 Abstract （816）   HTML （0）    PDF （2974KB）（36）       Knowledge map    Save Flavor is an important indicator of the edible quality of fresh corn. In order to explore the composition and content differences of volatile flavor compounds in different types of fresh corn，in this study， 6 different types of fresh corn varieties including NKN 323 were used as materials， and electronic nose technology and gas chromatography ion mobility spectrometry （GC-IMS） technology were used to detect the volatile flavor compounds in fresh corn to evaluate the contribution of volatile flavor compounds to the overall flavor of fresh corn by relative odor activity value （ROAV）. The results showed that a total of 34 volatile flavor compounds were identified， including 10 esters （34.68%~26.74%）， 8 aldehydes （28.46%~21.22%）， 6 alcohols （10.33%~6.04%）， 5 ketones （14.74%~9.87%）， 2 ethers （10.05%~6.94%） and 3 other compounds. GC-IMS fingerprints showed that the difference of volatile flavor compounds content important which caused the difference of characteristic flavor of different types of fresh corn. 5 common key aroma components and 23 different characteristic flavor compounds of fresh corn were identified by ROAV. The total release of volatile flavor compounds of different types of varieties was NKN323， followed by JKT608.Isovaleraldehyde contributed the most to the overall flavor of NKN336， JZN219， JKN768 and JKT608， while 3-octanone contributed the most to the overall flavor of NKN323 and JKN2000. Above results provided theoretical basis for the analysis and evaluation of volatile flavor compounds in fresh corn and the precise control of characteristic flavor. Table and Figures | Reference | Related Articles | Metrics

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Impact of ExsA on the Pathogenicity of Aeromonas veronii Zihui CAI, Wenxin CHEN, Juanjuan LI, Hong LI, Xue CHI, Yanqiong TANG, Zhu LIU, Xiang MA Journal of Agricultural Science and Technology    2024, 26 (9): 129-136.   DOI: 10.13304/j.nykjdb.2024.0316 Abstract （776）   HTML （0）    PDF （1704KB）（19）       Knowledge map    Save Aeromonas veronii is an important pathogen in the co-occurrence of humans and fish, causing significant mortality in various aquatic organisms, posing a major threat to aquaculture and public health care. The type Ⅲ secretion system (T3SS) is one of the most critical virulence mechanisms in determining the pathogenicity of Aeromonas species. The transcription factor ExsA of AraC family serves as the main regulatory factor and activates T3SS assembly and effector protein secretion in response to changes in environmental conditions. In order to explore the effect of exsA on the pathogenicity of the A. veronii, this study constructed an exsA gene knockout strain using homologous recombination strategy and tested its biofilm formation, adhesion capabilities, as well as the cytotoxicity of the spent cultural supernatant. The results showed that the deletion of the exsA gene in A. veronii led to a significant decrease of its biofilm formation capability, its adhesion to ex vivo host tissues, and the cytotoxicity of the spent culture supernatants. Compared with the wild type, the ability of the exsA knockout strain to form biofilm was reduced by 1.5 folds. The adhesion to epithelial cells was not significantly changed in exsA knockout strain as compared with the wild type, while the adhesion to ex vivo mouse intestinal tissues decreased by 3.4 folds and the cytotoxicity of bacterial culture supernatants decreases by 1.2 folds. These results proved that exsA plays an important regulatory role in the virulence mechanism of bacterial pathogen, laying a solid foundation for further research on the function of T3SS in the pathogenic mechanism of A. veronii and provided clues for establishing strategies for the control of the aquatic infection. Table and Figures | Reference | Related Articles | Metrics

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Identification and Sensitivity to Fungicides of Pathogen Species of Hass Avocado Fruit Anthracnose Jinlin LIU, Yingying CHEN, Xiaomei LAN, Haiyong CHEN, Pengsheng LI, Bi QIN, Ye YANG Journal of Agricultural Science and Technology    2025, 27 (1): 138-146.   DOI: 10.13304/j.nykjdb.2023.0702 Abstract （723）   HTML （1）    PDF （3306KB）（21）       Knowledge map    Save To identify the pathogen of anthracnose in avocado and screen the suitable fungicides， fruit samples of Hass avocado suspected anthracnose disease from Danzhou city， Hainan province were sampled. And the pathogen was isolated and identified. The pathogenicity of strain was confirmed according to Koch’s postulate. And the sensitivity of pathogenic strain to 9 fungicides were evaluated in vitro using mycelium radial growth assay. The results showed that the 4 pathogenic strains were identified as Colletotrichum siamense according to the morphological and phylogenetic analyses of multi-locus sequences. All of 4 strains showed virulence and high pathogenicity to avocado fruits. Among 9 fungicides， prochloraz was the most effective with the mean effective inhibitory medium concentration （EC50） values of 0.11 mg·L-1， and azoxystrobin， difenoconazole， pyrazoxystrobin， penthiopyrad and carbendazim followed with mean EC50 values ranged from 2.09 to 4.29 mg·L-1. So these 6 above-mentioned fungicides exhibited strong inhibitory activities against the mycelial growth of C. siamense， which could be used as candidate agents for the control of avocado anthracnose. Above results should help in developing strategies for the management and control of anthracnose in avocado. Table and Figures | Reference | Related Articles | Metrics

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Optimization of Extraction Conditions for Volatile Components in Jujube Honey Based on Plackett-Burman Design and Response Surface Method Xue WANG, Xing ZHENG, Jiamiao REN, Yazhou ZHAO, Wenjun PENG Journal of Agricultural Science and Technology    2025, 27 (1): 181-192.   DOI: 10.13304/j.nykjdb.2023.0384 Abstract （722）   HTML （0）    PDF （3482KB）（92）       Knowledge map    Save In order to enhance the head space-solid phase micro extraction （HS-SPME） extraction efficiency of volatile components in honey， and for more accurate qualitative and quantitative analysis using gas chromatograph-mass spectrometer （GC-MS）， the total peak area of volatile components of the chromatogram in Ziziphus jujuba Mill honey was used as the index. Plaekett-Burman design and response surface methodology were used to optimize extraction conditions （extraction temperature， extraction time， sample amount， deionized water amount， NaCl additive amount， stirring speed）. The results showed that extraction temperature， extraction time and sample amount significantly impacted the total peak area in the chromatograms. The optimized extraction conditions were extraction temperature of 68.5 ℃， extraction time of 51.6 min and sample amount of 5.1 g. Under the optimal extraction conditions， the total peak area of chromatogram could reach 2.20×109 mAU·min-1. Above results provided certain technical guidance for the further profile analysis of volatile components in honey. Table and Figures | Reference | Related Articles | Metrics

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Development of Spatial Transcriptomics and Its Applications Yongzi CHEN, Hua WANG, Weixuan WANG Journal of Agricultural Science and Technology    2024, 26 (11): 23-31.   DOI: 10.13304/j.nykjdb.2024.0609 Abstract （626）   HTML （9）    PDF （1366KB）（59）       Knowledge map    Save Spatial transcriptomics is a disruptive biological technique that integrates molecular biology and spatial imaging technologies to reveal the spatial distribution of gene expression within tissues or cells. Through the analysis of transcriptomics data， the spatial expression patterns of genes at tissue or cellular level could be understanded comprehensively， and then the spatiotemporal characteristics of gene regulation in biological systems and their functional significance in biological processes could be deeply uncovered. This technology provided unprecedented assistance for the research in developmental biology， immunology， tumor research and agriculture fields etc， and provided support for understanding of key processes such as cell differentiation and crop development etc. This article detaily overviewed the development of spatial transcriptomics technology， data analysis， and its major applications in the fields of medicine and agriculture. It also identified the challenges existed in the field and offered insights into future development trends. Table and Figures | Reference | Related Articles | Metrics

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Differential Expression Paradigm of Chemoreceptor Genes Between Males and Females at Different Developmental Stages of Carposina sasakii Matsumura Qian ZHANG, Lina MEN, Yiran LI, Qiao LIU, Angie DENG, Xiaowen HU, Yuhong ZHANG, Zhiwei ZHANG, Wei ZHANG Journal of Agricultural Science and Technology    2024, 26 (8): 151-162.   DOI: 10.13304/j.nykjdb.2023.0338 Abstract （490）   HTML （1）    PDF （2139KB）（42）       Knowledge map    Save Carposina sasakii Matsumura is a notable fruit pest that damages many types of fruit trees. Behavior regulation technology has gradually gained attraction as a potential pest control method that is both environmentally conscious and effective. The insect behavioral mechanism of chemosensory perception is the basis of behavior regulation. A total of 242 olfactory genes were identified in the antenna transcriptome of the C. sasakii. Based on sequence alignment and functional background of similar genes in related species， 13 olfactory genes were selected and their potential recognition functions were analyzed using RT-qPCR. The results showed that 6 genes differentially expressioned between males and females at different developmental stages （ P<0.05）， which corresponded to the behavioral phenotypes of adults. According to the difference expression levels between male and female adults during newly emerged， mature unmated and mature mated， OBP11 might play a role in detecting pheromones and mating behavior， OR45 might correspond to host-seeking and oviposition activities and IR5 was associated with mating and oviposition behavior. Above results laid theoretical molecular foundation for the identification of C. sasakii pheromone and olfactory-related genes and supported the implementation of behavioral regulators against the olfactory perception mechanism of C. sasakii as a more environmentally sustainable pest control strategy. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on Key Application Scenario of Intelligent Orchard Jianwei WU, Lin ZHANG, Wengang ZHENG, Xiangyang QIN, Zhonggao WU, Xihong FANG, Yongquan WANG, Tiangang LU, Jian WANG, Xiangshu PIAO, Fang WANG, Qiaoyun YUE, Haihe ZHANG, Jing JI, Xiaoman CONG, Qifeng LI Journal of Agricultural Science and Technology    2025, 27 (3): 12-23.   DOI: 10.13304/j.nykjdb.2024.0816 Abstract （487）   HTML （14）    PDF （6800KB）（107）       Knowledge map    Save Intelligent orchards is a new production mode that deeply integrates modern information technology and intelligent equipment with all aspects of orchard production， achieving quantitative decision-making， precise input， digital and intelligent management. To enhance the digitalization level of the fruit industry， focusing on the latest research progress in key application scenarios of intelligent orchards，this paper analyzed the strategic position and layout significance of intelligent fruit industry in the process of agricultural modernization， and proposed several suggestions to promote the development of intelligent orchards based on the current research and application status in China. It also detaily elaborated the design and establishment of a intelligent orchard scenario in Beijing， which encompassing integrated environmental monitoring of the orchard’s “sky-ground” environment， integrated water and fertilizer input， machine-friendly intelligent equipment and a comprehensive management service platform. It aimed to create an efficient collaborative ecosystem from multiple dimensions， point the way for the future development of intelligent orchards， inject new vitality into the deep integration of rural revitalization and digital agriculture， and promote the improvement， efficiency and high-quality development of the fruit industry. Table and Figures | Reference | Related Articles | Metrics

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Genetic Diversity Analysis of 255 Maize Germplasm Resources Based on 10K SNP Chip Shan GAO, Xiaocui YAN, Nan WANG, Mengjie ZHANG, Youpeng LI, Wenda DIAO, Huijun DUAN Journal of Agricultural Science and Technology    2024, 26 (8): 20-33.   DOI: 10.13304/j.nykjdb.2023.0801 Abstract （437）   HTML （13）    PDF （2016KB）（164）       Knowledge map    Save Maize （Zea mays L.） is one of the world’s top three food crops and an important feed crop. Analyzing the agronomic phenotypic traits and genotypic genetic diversity of maize germplasm resources has important guiding significance for crop breeding and production. A natural population composed of 255 germplasm resources was used as the research material， and the genotyping was obtained by whole genome scanning using 10K SNP liquid chip. Phenotypic clustering and genetic diversity analysis were performed using VCF2PCA cluster and Mega11 software. The results showed that 14 139 high quality SNP sites were detected， which were distributed on 10 chromosomes of maize. The polymorphisms ranged from 0.05 to 0.50， with an average of 0.29. Polymorphism information content （PIC） varied from 0.04 to 0.38 with an average of 0.24. 104 SNPs with allele frequency difference were more than 0.30 between tropical and temperate populations. Through population genetic structure analysis， principal component analysis and cluster analysis， 255 samples were mainly divided into temperate and tropical subgroups. These results identified the genetic diversity and relationships among 255 germplasm resources from temperate and tropical maize， laying the foundation for subsequent maize germplasm improvement and innovation. Table and Figures | Reference | Related Articles | Metrics

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Exploration on Intelligent Path of Animal Husbandry from Perspective of Metauniverse Fan YANG, Qingning LIN, Qi WANG, Shiping MAO Journal of Agricultural Science and Technology    2024, 26 (8): 1-8.   DOI: 10.13304/j.nykjdb.2023.0406 Abstract （428）   HTML （9）    PDF （484KB）（89）       Knowledge map    Save The metaverse， as a prominent paradigm in the future digital world， presents a viable framework for the transformation and advancement of the livestock industry. Grounded in the metaverse perspective， this paper explored the pathway for the implementation of intelligent livestock farming， and discussed the current state and existing challenges in the intelligentization of the livestock industry. It proposed an intelligent pathway supported by technologies such as blockchain， artificial intelligence （AI） and the internet of things （IoT）. The realization pathway for intelligent livestock farming primarily encompassed the following aspects： firstly， implementing intelligent livestock farming through the utilization of big data and IoT technologies， involving systematic data collection and precise analysis to optmize production efficiency and monitor the health status of animals； secondly， adopting blockchain technology to record the origin， production process， and distribution chain of livestock products， thereby establishing a fully traceable product quality assurance system； thirdly， utilizing virtual education platforms for livestock industry training and education， assisting users in acquiring knowledge and skills within a virtual environment， ultimately enhancing technological innovation capabilities； and finally， leveraging the dual roles of government and market to activate elements such as funds， talent and technology， thereby propelling technological innovation and industry transformation and upgrading in the livestock sector. Based on these considerations， it was anticipated that the guidance of digital technology should lead to the high-quality development of the livestock industry. Reference | Related Articles | Metrics

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Current Situation， Problems and Countermeasures of Strontium Natural Drinking Mineral Water Industry in China Kunzhen WANG, Xin RU, Guohui SHEN, Yanting GU, Mingying REN, Juan HAN, Yanzhi GUO Journal of Agricultural Science and Technology    2025, 27 (4): 12-21.   DOI: 10.13304/j.nykjdb.2024.0625 Abstract （402）   HTML （6）    PDF （515KB）（87）       Knowledge map    Save Since the reform and opening-up， China’s natural mineral water industry has experienced rapid development， with an increasing diverse range of products. In particular， mineral water enriched with specific nutritional elements has gained significant popularity among consumers. The intake of the trace element strontium has been shown to play effective roles in improving osteoporosis， preventing cardiovascular diseases， and promoting glucose and lipid metablism. However， the strontium intake of Chinese residents has not yet reached the recommended levels. Strontium-rich natural mineral water serves as an important source of strontium supplementation for the human body， indicating a significant market potential in the future. Considering the current state and development trends of China’s natural mineral water industry， the market supply potential of existing strontium-rich natural mineral water resources was analysed. It addressed the development bottlenecks faced by different categories of strontium-rich natural mineral water available in the market and offered suggestions regarding price， quality and labeling， which aimed to effectively foster the sustainable development of China’s strontium-rich natural mineral water industry. Table and Figures | Reference | Related Articles | Metrics

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Progress in Creation of Rice Male Sterile Lines by CRISPR/Cas9 Gene Editing Technology Dabing YANG, Liang HU, Xueshu DU, Bingliang WAN, Mingyuan XIA, Huaxiong QI, Jinbo LI Journal of Agricultural Science and Technology    2025, 27 (3): 24-34.   DOI: 10.13304/j.nykjdb.2023.0460 Abstract （392）   HTML （8）    PDF （519KB）（101）       Knowledge map    Save CRISPR/Cas9 （clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9） gene editing technology could modify specific gene to achieve targeted improvement of interest traits， which has promising application prospect in crop genetic improvement. Hybrid rice breeding system based on male sterile lines is an important way to utilize rice heterosis. Editing rice male sterile genes can create new male sterile germplasm， which is helpful to enrich the genetic resources of hybrid rice sterile lines and take good advantage of rice heterosis. In this paper， the research progress of creating male sterile lines of rice by CRISPR/Cas9 gene editing technology was reviewed， and the future research direction of male sterile lines of rice was prospected， aiming to provide reference for the innovation and development of rice breeding technology. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on Effects of Medium and Trace Elements on Yield and Quality of Rice Liang SUN, Yi XU, Qin CAI, Jinghao GUO, Can ZHAO, Baowei GUO, Zhipeng XING, Zhongyang HUO, Hongcheng ZHANG, Yajie HU Journal of Agricultural Science and Technology    2024, 26 (8): 9-19.   DOI: 10.13304/j.nykjdb.2024.0141 Abstract （384）   HTML （26）    PDF （726KB）（203）       Knowledge map    Save As essential nutrients for rice growth and development， medium and trace elements are the components and activators of enzyme systems， and also participate in a series of important physiological processes， such as photosynthesis， respiration， hormone synthesis and disease defense， which play vital roles in the formation of yield and quality for rice. Therefore， the effects of the absorption， accumulation and metabolic pathways of medium and trace elements， such as magnesium， zinc， seleniumon and iron， on rice yield and quality were reviewed， the challenges faced by the medium and trace elements in rice cultivation were analyzed， and the importance of medium and trace elements and the research direction in sustainable agriculture in the future were discussed， which provided theoretical basis for achieving high yield and high quality of rice. Table and Figures | Reference | Related Articles | Metrics

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Research on International Comparison and Development Strategy of Competitiveness of China’s Oilseed Industry Chang CHEN, Dan GUO, Qinghong BAI, Yang ZHANG, Maolin YAN Journal of Agricultural Science and Technology    2025, 27 (4): 1-11.   DOI: 10.13304/j.nykjdb.2023.0763 Abstract （368）   HTML （25）    PDF （1007KB）（91）       Knowledge map    Save In order to further enhance the competitiveness of China’s oilseed industry and promote its high-quality development， the data of oilseed production， consumption and trade since 2000 were selected， and the competitiveness of China’s oilseed industry from the perspectives of absolute advantage， comparative advantage and enterprise competitiveness were systematically analyzed， the main reasons affecting the competitiveness of China’s oilseed industry were discussed by calculating the contribution rate of oilseed planting technology and the trade competitiveness index. The results showed that China’s oilseed industry was at a disadvantage in all the aspects of absolute advantage， comparative advantage and enterprise competitiveness， and the limiting factors were insufficient resource endowment， low contribution of technological progress， imperfect symbiotic development of the upstream and downstream of the industrial chain and unfavorable domestic and foreign agricultural policy environment. Based on this， targeted opinions and suggestions were put forward from multiple perspectives such as resource development， technological breakthrough and policy support， which provided data references and technical supports for the formulation of the national grain and oilseed development strategy. Table and Figures | Reference | Related Articles | Metrics

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Analysis of Physiological Characteristics About ABA Alleviating Foxtail Millet Seedling Stage Under Drought Stress ling QIN, Yanke WANG, Erying CHEN, Yanbing YANG, Feifei LI, Mengyuan ZHANG, Yanan GUAN Journal of Agricultural Science and Technology    2025, 27 (4): 36-44.   DOI: 10.13304/j.nykjdb.2023.0876 Abstract （359）   HTML （5）    PDF （2515KB）（148）       Knowledge map    Save In order to study the effects of drought stress on physiological characteristics of foxtail millet at seedling stage and the alleviating effect of exogenous abscisic acid （ABA）， different drought tolerant foxtail millet varieties Jigu 22 and Lugu 1 were used as materials to conduct pot experiment. The effects of 20% PEG6000 simulated dehydration stress at seedling stage and spraying 0.1 mmol L-1 ABA on morphological character， activities of antioxidant enzymes and ascorbate peroxidase （APX）， contents of malondialdehyd （MDA）， proline （Pro）， soluble sugar （SS） and soluble protein （SP） in 2 different foxtail millet varieties were analyzed. The results showed that the relative seedling height of Jigu 22 after drought stress for 4 d was 81.49%，which was higher than that of Lugu 1. MDA content in leaves of the 2 varieties increased after drought stress. On the 7th day of stress， MDA content in Jigu 22 increased by 3.03%， and that in Lugu 1 increased by 33.61% compared with control. The MDA content was significantly reduced after application of exogenous ABA. Exogenous ABA promoted the activities of superoxide dismutase（SOD）， peroxidase（POD）， catalase（CAT） and APX. Osmotic adjustment substances Pro， SS and SP were synthesized in large amounts to maintain osmoregulation and alleviate the damage of drought stress on foxtail millet seedlings. The effect of exogenous ABA on the drought-tolerant variety Jigu 22 was more obvious under drought stress. The effect of exogenous ABA on Lugu 1 was relatively weak， especially on the 7th day of drought stress， and the physiological and growth characteristics recovered slowly. In summary， ABA could effectively alleviate the damage of physiological function and the effect of drought stress on seedling growth of foxtail millet. Different drought resistance varieties foxtail millet had different sensitivity to ABA. The varieties with strong drought resistance were more sensitive to ABA， the mitigation effect of ABA on drought was more obvious. Above results provided theoretical bases for revealing the physiological mechanism of foxtail millet response to drought stress and the application of exogenous ABA. Table and Figures | Reference | Related Articles | Metrics

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Discovering of QTLs and Candidate Genes Related to Rice Heading Period Traits Based on BSA-seq Ronghua WEI, Ming YIN, Wensheng WANG, Yanru CUI Journal of Agricultural Science and Technology    2024, 26 (9): 12-24.   DOI: 10.13304/j.nykjdb.2024.0216 Abstract （356）   HTML （21）    PDF （6935KB）（114）       Knowledge map    Save As one of the important traits of rice， rice heading date is closely related not only to the rice growth period but also to rice yield， quality and stress resistance， which determines the planting area and seasonal adaptability of rice varieties. Therefore， the location of rice heading date related genes plays an important role in rice production. In this study， HQ20， Jin 23B and their F4 population constructed were used as the materials. According to the heading stage of the rice in the population， it was divided into 3 levels including early， middle and green plant （not heading）， and 3 mixed pools of early， middle and green were constructed. The bulked-segregant analysis sequencing （BSA-seq） method was used to discover rice heading period genes. The results showed that the comparison rate between BSA-seq results and the reference genome was over 94%， and the 4× genome coverage rate was over 80%. Based on this， when the confidence level was 0.95， by SNP-index algorithm 17 SNPs located on 4 chromosomes （6， 7， 8 and 9） were obtained which had consistent association intervals with indel. Further analysis of candidate genes in the region found that 61 genes had non-synonymous mutations. GO and KEGG enrichment analysis showed that pathways closely related to rice heading stage included UDP-glycosyltransferase activity， positive regulation of cell cycle G2/M phase transition， and phytohormone signal transduction. Finally， combining haplotype analysis in the 3K rice database and published expression data， 3 key candidate genes for heading stage were discovered including LOC_Os07g22720， LOC_Os07g23740 and LOC_Os08g07200. The above results laid the foundation for genetic improvement of rice heading period traits and analysis of genetic basis. Table and Figures | Reference | Related Articles | Metrics

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Screening and Evaluation of Drought Resistance Index for Maize Hybrids During Seedling and Germination Stages Yuan HE, Xiaotong GU, Liqing FENG, Huijun DUAN, Yongsheng TAO Journal of Agricultural Science and Technology    2024, 26 (10): 30-40.   DOI: 10.13304/j.nykjdb.2023.0809 Abstract （352）   HTML （9）    PDF （2888KB）（69）       Knowledge map    Save To screen the drought resistance indicators of the main maize hybrids for relatively large acreages at germination and seedling stages， the released maize hybrids were used as test materials. 2 treatments were set up including control and drought stress （PEG-6000 with mass fraction of 20%）. Germination rate， germination potential and germination index were measured at germination stage， and MDA content， chlorophyll content， root dry weight， canopy dry weight， plant dry weight， plant fresh weight， root to shoot dry weight ratio and dry to fresh ratio were measured from the seedling stage to the three leaf one heart stage. A comprehensive evaluation of drought resistance and screening of drought resistance indicators for maize varieties were conducted using drought resistance coefficient， weighted drought resistance coefficient， principal component analysis， membership function analysis and other methods. The results showed that the germination rate， germination potential and germination index of maize hybrids showed different degrees of decline during the germination period. The drought resistance coefficients of germination rate, germination potential and germination index varied widely, ranging from 7.33 to 97.78, 14.81 to 97.69 and 33.25 to 98.59, respectively. Based on the drought coefficient and cluster analysis, the hybrids were divided into 3 categories: drought resistant, moderate and sensitive, which correspond to the total comprehensive evaluation values (Ti) of 0.00~0.35, 0.53~0.66 and 0.77~0.96, respectively. There were 11 sensitive varieties during the germination period, including Zhengdan 958 (V1), Jingdan 28 (V3) and Huanong 138 (V5) etc. There were 4 drought resistant varieties, including Xianyu 335 (V2), Jingke 968 (V4) and Xianyu 047 (V7) etc. The MDA and chlorophyll contents in the leaves of the maize varieties at the seedling stage were significantly increased compared to the control (CK), while the other 6 traits showed varying degrees of change. Based on principal component analysis and cluster analysis of drought resistance coefficient and weighted drought resistance coefficient, the varieties were grouped into 3 categories: drought resistant, moderate and sensitive. The mean values of their total comprehensive evaluation values (Ti) were 0.30, 0.49 and 0.55, respectively. There were 5 sensitive varieties during the seedling stage, including Xianyu 1140 (V8), MC817 (V16) and Zhengdan 958 (V1) etc. There were 7 drought resistant varieties, including Jingke 968 (V4), Xianyu 047 (V7) and Nongda 372 (V9) etc. Therefore, the identification of drought resistance at the germination and seedling stages of maize requires the application of comprehensive evaluation methods to effectively assess drought resistance, especially for maize production in Huanghuaihai region with scarce water resources and available water resources were severely limited. It was more important to study the drought resistance evaluation of maize varieties at the seedling and germination stages under the single seed sowing mode. Table and Figures | Reference | Related Articles | Metrics

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Recent Advances of CRISPR-Cas System in Genome Editing of Bacillus subtilis Zhikang SUN, Liqun LI, Jie HAO, Han WU, Na WU, Chao ZHENG, Qiang JI, Xuanwen LI, Chen CHEN Journal of Agricultural Science and Technology    2025, 27 (2): 24-32.   DOI: 10.13304/j.nykjdb.2023.0260 Abstract （347）   HTML （12）    PDF （757KB）（108）       Knowledge map    Save Bacillus subtilis is a food safety microorganism， which has been widely used in industrial fermentation. CRISPR （clustered regularly interspaced short palindromic repeats） mediated genome editing technology has played an important role in the research of microbial metabolic engineering with B. subtilis as the chassis cell. The immune response mechanism and classification of CRISPR-Cas system were introduced， as well as the 3 types of CRISPR-Cas9 genome editing in B. subtilis. It focused on summarizing the latest CRISPR development and design strategies， with a view to providing references for optimizing existing B. subtilis genome editing systems， thereby improving the industrial application potential of B. subtilis. Table and Figures | Reference | Related Articles | Metrics

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Response of GhCOMT28 to Drought Stress in Gossypium hirsutum Yixin CHEN, Xiubo YANG, Shijun TIAN, Cong WANG, Zhiying BAI, Cundong LI, Ke ZHANG Journal of Agricultural Science and Technology    2025, 27 (4): 45-56.   DOI: 10.13304/j.nykjdb.2024.0059 Abstract （344）   HTML （6）    PDF （6403KB）（231）       Knowledge map    Save Melatonin， as an indoleamine compound， has a wide range of roles in regulating plant growth and development and resisting adversity stress. COMT （caffeic acid O-methyltransferase） is an O-methyltransferase that plays an important role in the phenylpropane metabolic pathway and is a key enzyme in melatonin synthesis. There are 57 GhCOMTs homologous genes in terrestrial cotton， among which GhCOMT28 and GhCOMT55 have the highest homology with Arabidopsis thalianaAtCOMT. In this study， GhCOMT28 was used as an example to parse its biological function， and GhCOMT28 and GhCOMT55 had the highest expression in the roots and stems of cotton， and GhCOMT28 was localized in the cells in the cytoplasm of cell. GhCOMT28 mRNA was induced by drought stress， and silencing of GhCOMT28 （TRV2：GhCOMT28） resulted in reduced melatonin levels in leaves， and the expression of superoxide dismutase （SOD）， peroxidase （POD）， catalase （CAT） and other antioxidant enzymes decreased， free radicals accumulation increased， and plant drought tolerance decreased. Overexpression of GhCOMT28 （35S：GhCOMT28-GFP） in Arabidopsis significantly improved plant drought tolerance. This study revealed that GhCOMT28 had an important role in regulating drought tolerance in cotton seedlings and had potential use for drought-tolerant variety improvement. Table and Figures | Reference | Related Articles | Metrics

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Reaearch on the Application of AI for Science in Agricultural Field Song FANG, Lihua JIANG, Jingjun CAO, Xiao WANG, Minghui QIU, Xiaoyi TIAN Journal of Agricultural Science and Technology    2024, 26 (10): 1-10.   DOI: 10.13304/j.nykjdb.2024.0473 Abstract （343）   HTML （13）    PDF （1713KB）（108）       Knowledge map    Save In recent years， artificial intelligence （AI）， driven by the three engines of algorithm， data and computing power， has developed rapidly， and achieved many remarkable achievements in frontier fields， such as AlphaFold3， nuclear fusion intelligent control， and novel coronavirus drug design. AI-driven scientific research （AI for Science， AI4S） has solved the bottleneck of scientific data analysis with high dimensions， large scale and span， and limited scientific experiments that restrict large-scale interdisciplinary scientific research activities， and promotes scientific research towards a new model with “platform collaboration” as the main feature. This paper reviewed the international situation of AI4S， the current development status of China’s smart agriculture and the practical dilemma， and combined literature， statistical data， and investigation case analysis to put forward the practical path to promote AI4S application in China’s agricultural development. AI4S would become a powerful engine to transform agricultural production from the traditional model of “looking at the sky， looking at the land and looking at the crops” to models such as intelligent perception， intelligent decision-making and visual management， and promote scientific research from the single-fighting “small farmer’s workshop” model to the platform scientific research of “Android model”. Researchers could share basic computing power， models， algorithms， databases and knowledge bases. On this platform， the research and development and application of the whole life cycle of the agricultural industry chain would be focused on， and scientific research innovation and application of results be accelerated through the “snowball effect”. Using artificial intelligence technology to realize digitalization， networking and intelligence of agricultural production， and support online iteration of theory-experiment. It is also necessary to improve the high-quality agricultural science digital resource system， appropriately advance AI key technologies and infrastructure， optimize the cross-innovation research ecology under the new paradigm， formulate agricultural data management norms， strengthen policy creation and supporting policy mechanisms to break through data barriers and to promote artificial intelligence + agriculture， and to strengthen agricultural science and technology innovation from the source. Table and Figures | Reference | Related Articles | Metrics

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Synergistic Effect of Combination of Antagonists and Chemical Fungicides on Atractylodes macrocephala Blight Disease Jiao WANG, Wenqiao LI, Fengying SHEN, Yaning LI, Daqun LIU Journal of Agricultural Science and Technology    2025, 27 (4): 133-139.   DOI: 10.13304/j.nykjdb.2024.0269 Abstract （339）   HTML （0）    PDF （1116KB）（73）       Knowledge map    Save In order to investigate the synergistic effect of different antagonistic bacteria， Streptomyces and their combination with chemical fungicides on Atractylodes macrocephala blight disease， the compatibility of different antagonists and chemical fungicides was measured by dilution plate and drug-containing plate method at first. Then the plate confrontation method and growth rate method were used to evaluate the inhibition effect of antagonists compound solution and antagonists+chemical fungicides compound on Phytophthora sansomeana AMPH-1， which was the pathogen of A. macrocephala blight disease. The results showed that the Bacillus BZX-2， BZX-11 and X-119 strains had good compatibility with dimethomorph. The combination of BZX-2+BZX-11+X-119 had the best inhibition effect， and the inhibition rate reached 75.45%， which was lower than that of the treatment using X-119 alone. When the ratio of antagonist combination of Bacillus strains and dimethomorph was 1∶2， the inhibition effect reached 68.61%， which increased 16.83% and 13.59% compared with dimethomorph treatment and antagonists treatment， respectively. By the pot experiment in greenhouse， it was found that the greenhouse control efficiency of BZX-2+BZX-11+X-119 combination with equal proportion mixing was 72.72% on A. macrocephala blight disease， which was 45.45 percentage points higher than that of BZX-2 treatment. Above results provided effective basis for the new strategy of A. macrocephala blight disease control. Table and Figures | Reference | Related Articles | Metrics

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Analysis and Comparative Characteristics of Codon Preference in Chloroplast Genome of Polygonum genus Qilin LIU, Jinze LI, Jiayu DING, Shuoli WANG, Ziming CHENG, Kangyuan GU, Guangyan FENG Journal of Agricultural Science and Technology    2025, 27 (4): 87-98.   DOI: 10.13304/j.nykjdb.2024.0090 Abstract （335）   HTML （4）    PDF （7643KB）（196）       Knowledge map    Save To explore the structure， function and evolutionary characteristics of chloroplast genome of Polygonum plants， the software of CodonW， SPSS and online programs Galaxy， Genepioneer Bioinformatics Cloud， mVISTA， GENE DENOVO and other tools were used to analyze the codon usage preferences and phylogenetic relationships of the chloroplast genomes of 28 Polygonum plants. The results showed that the codon adaptation index （CAI） and effective number of codon （ENC） in chloroplast genomes of 28 species of Polygonum plants were low， which indicated weak codon usage preference and being mainly influenced by natural selection. The third base of codon mainly ended in A/U， and 9 optimal codons of chloroplast genome all ended in A/U. The correlation analysis between the GC content at different positions of codon， ENC and GC3s， GC3， GC2 revealed that codon preference in chloroplast genome of Polygonum was weak and mainly influenced by the third base composition， while the length of the chloroplast gene sequence had less influence on base composition and codon preference. The phylogenetic tree based on the relative synonymous codon usage （RSCU） was consist with those besed on matk and rbcl gene sequences， 28 species of Polygonum plants were classified into 3 groups， but there were differences in each group， such as P. maackiana was same group with P. tinctoria in phylogenetic tree based on matk gene， while was same group with P. posumbu in phylogenetic tree based on rbcl gene. Above results provided a reference for the utilization， phylogeny， and chloroplast genetic engineering of Polygonum plants. Table and Figures | Reference | Related Articles | Metrics