Evaluation of the Growth-promoting Effect of Trichoderma harzianum on Tobacco and Its Induced Resistance to Black Shank Disease

doi:10.13304/j.nykjdb.2024.0225

Abstract

Abstract:

To investigate the growth-promoting effects of Trichoderma harzianum on tobacco and its induced resistance mechanisms against tobacco black shank disease caused by Phytophthora nicotianae， 5 Trichoderma strains were screened. The results showed that the strain T. harzianum ACCC 32808 （TH32808） was demonstrated the strongest inhibition （62.00%） among 5 strains of Trichoderma. The growth-promoting effects of TH32808 on different tobacco varieties and its induced resistance against tobacco black shank disease were assessed. The results showed that， under pot condition， TH32808 exhibited different ability on promoting the growth of different tobacco varieties. Under TH32808 treatment， the fresh weight of Honghuadajinyuan increased by 8.30%， the control efficacy against black shank disease in Honghuadajinyuan was 51.74%. In Honghuadajinyuan and K326， the catalase activity peaked on the 4th day， reaching 1.42 and 2.15 times that of CK， the peroxidase activity peaked on the 2nd day， being 2.49 and 2.29 times that of CK， the superoxide dismutase activity peaked on the 4th day， reaching 1.17 and 1.15 times that of CK， respectively. Moreover， TH32808 could induce resistance against black shank disease， key genes involved in the metabolic pathways of salicylic acid， jasmonic acid and ethylene， such as NPR1， PDF1.2， EFE and NtSGT1， all exhibited an upregulation trend. Above results provided fungal resources and theoretical basis for the biological control and induced resistance research against black shank disease in tobacco.

Key words: Trichoderma harzianum, growth-promoting effect, black shank disease in tobacco, induced resistance, biological control

摘要：

为研究哈茨木霉菌（Trichoderma harzianum）对烟草促生作用及由烟草疫霉菌（Phytophthora nicotianae）引起的烟草黑胫病的诱导抗性分子机制，对5株木霉菌株进行筛选。结果表明，哈茨木霉ACCC 32808（TH32808）菌株对烟草疫霉菌的抑制性较强，抑制率为62.00%。进一步研究TH32808对不同烟草品种的促生作用及对烟草黑胫病的生物防治诱导抗性，结果表明，在盆栽条件下，TH32808对不同烟草品种的促生作用不同，其中红花大金元的植株鲜重显著增加8.30%，对黑胫病害的防治效果可达51.74%；红花大金元和K326的过氧化氢酶活性在第4天时较高，分别为对照的1.42和2.15倍，过氧化物酶活性在第2天时较高，分别为对照的2.49和2.29倍，超氧化物歧化酶活性在第4天较高，分别为对照的1.17和1.15倍。TH32808可诱导烟草对黑胫病的抗性，关键信号分子水杨酸、茉莉酸和乙烯代谢途径的关键基因NPR1、PDF1.2、EFE和NtSGT1均呈上调表达趋势。研究结果为烟草黑胫病的生物防治和诱导抗性研究提供了生防真菌资源和理论依据。

关键词: 哈茨木霉, 促生作用, 烟草黑胫病, 诱导抗性, 生物防治

CLC Number:

S572

Junjia CHANG, Jiaxin GAI, Gang TAO, Zhuanlonghai MO. Evaluation of the Growth-promoting Effect of Trichoderma harzianum on Tobacco and Its Induced Resistance to Black Shank Disease[J]. Journal of Agricultural Science and Technology, 2024, 26(10): 168-176.

常峻嘉, 盖佳鑫, 陶刚, 莫转龙海. 哈茨木霉菌对烟草的促生及其黑胫病的诱导抗性评价[J]. 中国农业科技导报, 2024, 26(10): 168-176.

Figures/Tables 9

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基因 Gene	上游引物 Forward primer （5’-3’）	下游引物 Reverse primer（5’-3’）
β-actin	ATGCCTATGTGGGTGACGAAG	TCTGTTGGCCTTAGGGTTGAG
NPR1	ACATCAGCGGAAGCAGTAG	GTCGGCGAAGTAGTCAAAC
NtPDF1.2	GGAAATGGCAAACTCCATGCG	ATCCTTCGGTCAGACAAACG
EFE	ATTTATCCAGCACCAGCTCT	GCTTCAAACCTTGGCTCCT
NtSGT1	TCCACACTCGCCATCCTGAA	GTGCCGCAAATTCAACTCTG

基因 Gene	上游引物 Forward primer （5’-3’）	下游引物 Reverse primer（5’-3’）
β-actin	ATGCCTATGTGGGTGACGAAG	TCTGTTGGCCTTAGGGTTGAG
NPR1	ACATCAGCGGAAGCAGTAG	GTCGGCGAAGTAGTCAAAC
NtPDF1.2	GGAAATGGCAAACTCCATGCG	ATCCTTCGGTCAGACAAACG
EFE	ATTTATCCAGCACCAGCTCT	GCTTCAAACCTTGGCTCCT
NtSGT1	TCCACACTCGCCATCCTGAA	GTGCCGCAAATTCAACTCTG

处理 Treatment	病原菌半径 Pathogen radius/mm	抑制率 Inhibition rate/%
TH 32808	7.17±0.21 d	62.00
ACCC 32809	7.84±0.33 bcd	58.45
ACCC 31649	8.49±0.65 bc	54.96
ACCC 32826	7.36±0.16 cd	60.07
ACCC 32969	8.83±0.43 b	53.16
CK1	18.86±0.44 a	—

处理 Treatment	病原菌半径 Pathogen radius/mm	抑制率 Inhibition rate/%
TH 32808	7.17±0.21 d	62.00
ACCC 32809	7.84±0.33 bcd	58.45
ACCC 31649	8.49±0.65 bc	54.96
ACCC 32826	7.36±0.16 cd	60.07
ACCC 32969	8.83±0.43 b	53.16
CK1	18.86±0.44 a	—

品种 Variety	处理 Treatment	株高 Plant height/cm	茎径 Stem diameter/mm	鲜重 Fresh weight/g	干重 Dry weight/g
红花大金元 Honghuadajinyuan	CK2	75.58±6.54 a	5.05±0.11 ab	12.99±0.37 b	1.20±0.04 b
	TH32808	72.36±4.67 a	5.23±0.24 a	13.93±0.68 ab	1.16±0.06 b
	ACCC31649	66.19±6.32 ab	4.85±0.15 ab	14.05±0.46 a	1.52±0.04 a
K326	CK2	49.82±2.47 bc	4.46±0.19 b	9.47±0.42 c	0.76±0.44 cd
	TH32808	40.86±3.71 c	4.83±0.31 ab	9.59±0.41 c	0.74±0.02 d
	ACCC31649	45.23±4.61 c	4.52±0.11 b	10.25±0.56 c	0.83±0.05 c