Progress in Creation of Rice Male Sterile Lines by CRISPR/Cas9 Gene Editing Technology

doi:10.13304/j.nykjdb.2023.0460

Abstract

Abstract:

CRISPR/Cas9 （clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9） gene editing technology could modify specific gene to achieve targeted improvement of interest traits， which has promising application prospect in crop genetic improvement. Hybrid rice breeding system based on male sterile lines is an important way to utilize rice heterosis. Editing rice male sterile genes can create new male sterile germplasm， which is helpful to enrich the genetic resources of hybrid rice sterile lines and take good advantage of rice heterosis. In this paper， the research progress of creating male sterile lines of rice by CRISPR/Cas9 gene editing technology was reviewed， and the future research direction of male sterile lines of rice was prospected， aiming to provide reference for the innovation and development of rice breeding technology.

Key words: gene editing, rice, male sterile line, breeding application

摘要：

CRISPR/Cas9（clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9）基因编辑技术可以对特定基因进行改造，从而实现目标性状的定向改良，在作物遗传改良中具有良好的应用前景。而基于雄性不育系建立的杂交水稻育种体系是水稻杂种优势利用的重要途径，编辑水稻雄性不育基因能够定向创制新的雄性不育种质，有助于丰富杂交水稻不育系遗传资源，更好地利用水稻杂种优势。综述了CRISPR/Cas9基因编辑技术创制水稻雄性不育系的研究进展，展望了基因编辑水稻雄性不育系未来研究方向，以期为杂交水稻育种技术的创新与发展提供参考。

关键词: 基因编辑, 水稻, 雄性不育系, 育种应用

CLC Number:

S511

Dabing YANG, Liang HU, Xueshu DU, Bingliang WAN, Mingyuan XIA, Huaxiong QI, Jinbo LI. Progress in Creation of Rice Male Sterile Lines by CRISPR/Cas9 Gene Editing Technology[J]. Journal of Agricultural Science and Technology, 2025, 27(3): 24-34.

杨大兵, 胡亮, 杜雪树, 万丙良, 夏明元, 戚华雄, 李进波. CRISPR/Cas9基因编辑技术创制水稻雄性不育系的研究进展[J]. 中国农业科技导报, 2025, 27(3): 24-34.

Figures/Tables 4

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	WU H X, LI B H, XIANG Y, et al.. Effects of temperature and photoperiod on the fertility of low temperature sensitive genic male sterile rice [J]. Acta Ecol. Sin., 2003, 23(3):463-470.
94	徐孟亮,张俊,龚曼,等.一个反向水稻两用核不育系育性对温度与光周期的反应[J].湖南师范大学自然科学学报(自然科学版),2010,33(4):85-88.
	XU M L, ZHANG J, GONG M, et al.. Response of fertility of a new reverse P(T)GMS line to temperature and photoperiod in rice [J]. J. Hunan Norm. Univ. (Nat. Sci.), 2010, 33(4):85-88.
95	江建华,倪金龙,吴爽,等.聚合水稻温敏核不育基因和反温敏核不育基因创制永久核不育系[J].中国水稻科学,2017,31(4):371-378.
	JIANG J H, NI J L, WU S,et al.. Development of permanent genic male sterile line by pyramiding thermos-sensitive male sterile genes and reverse temperature induced genic male sterile genes in rice (Oryza sativa L.) [J]. Chin. J. Rice Sci., 2017, 31(4):371-378.
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基因 Gene	染色体 Chromosome	功能 Function	编码蛋白 Encoding protein	参考文献 Reference
OsMSP1	1	大、小孢子发生相关 Related to macrosporogenesis and microsporogenesis	富亮氨酸重复类受体蛋白激酶 Leucine-rich-repeat receptor-like kinase	［17］
TIP2	1	绒毡层降解 Tapetum degeneration	bHLH转录因子 bHLH transcription factor	［18］
OsMADS3	1	花器官发育相关 Related to floral organ development	C类花同源异型转录因子 C-class floral homeotic transcription factor	［19］
DPW2	1	花药角质层、花粉壁合成 Anther cuticle and pollen wall formation	酰基转移酶 Acyltransferase	［20］
OsAPI5	2	绒毡层降解 Tapetum degeneration	凋亡抑制因子 Inhibitor of apoptosis	［21］
OsMTR1	2	绒毡层和小孢子发育 Tapetum and microspore development	分泌性糖蛋白 Secreted glycoprotein	［22］
TDR	2	绒毡层降解 Tapetum degeneration	bHLH转录因子 bHLH transcription factor	［23］
PTC2	2	绒毡层细胞持续存在 Persistence of tapetum cells	AT-hook DNA结合蛋白 AT-hook DNA binding protein	［24］
CYP704B2	3	花粉壁形成 Pollen wall formation	脂肪酸羟化酶 Fatty acid hydroxylase	［25］
DPW	3	花药及花粉外壁的合成 Synthesis of anther and pollen outer wall	脂酰基载体蛋白还原酶 Fatty acyl carrier protein reductase	［26］
OsTDF1	3	绒毡层降解 Tapetum degeneration	R2R3 MYB转录因子 R2R3 MYB transcription factor	［27］
OsDEX1	3	绒毡层降解、花粉壁发育 Tapetum degeneration and pollen wall development	钙离子结合蛋白 Calcium-binding protein	［28］
OsDGD2β	3	花粉外壁发育 Pollen outer wall development	双内酯甘油合酶 Dialactone glycerol synthase	［29］
TIP3	3	调控乌氏体和花粉外壁形成 Regulation of Ubisch bodies and pollen wall formation	PHD指蛋白 PHD finger protein	［30］
EAT1	4	绒毡层降解 Tapetum degeneration	bHLH转录因子 bHLH transcription factor	［31］
OsACOS12	4	孢粉素合成相关 Related to sporopollenin synthesis	酰基辅酶A合成酶 Acyl-CoA synthetase	［32］
OsABCG15	6	花粉外壁发育相关 Related to pollen outer wall development	ATP结合盒转运蛋白 ATP-binding cassette transporters	［33］
OsMADS16	6	花器官发育相关 Related to floral organ development	B类花同源异型转录因子 B-class floral homeotic transcription factor	［34］
MIL1	7	孢子母细胞减数分裂、花药壁细胞的分化 Meiosis of spore mother cells and differentiation of anther wall cells	CC型谷氧还蛋白 CC-type glutaredoxin	［35］

基因 Gene	染色体 Chromosome	功能 Function	编码蛋白 Encoding protein	参考文献 Reference
OsMSP1	1	大、小孢子发生相关 Related to macrosporogenesis and microsporogenesis	富亮氨酸重复类受体蛋白激酶 Leucine-rich-repeat receptor-like kinase	［17］
TIP2	1	绒毡层降解 Tapetum degeneration	bHLH转录因子 bHLH transcription factor	［18］
OsMADS3	1	花器官发育相关 Related to floral organ development	C类花同源异型转录因子 C-class floral homeotic transcription factor	［19］
DPW2	1	花药角质层、花粉壁合成 Anther cuticle and pollen wall formation	酰基转移酶 Acyltransferase	［20］
OsAPI5	2	绒毡层降解 Tapetum degeneration	凋亡抑制因子 Inhibitor of apoptosis	［21］
OsMTR1	2	绒毡层和小孢子发育 Tapetum and microspore development	分泌性糖蛋白 Secreted glycoprotein	［22］
TDR	2	绒毡层降解 Tapetum degeneration	bHLH转录因子 bHLH transcription factor	［23］
PTC2	2	绒毡层细胞持续存在 Persistence of tapetum cells	AT-hook DNA结合蛋白 AT-hook DNA binding protein	［24］
CYP704B2	3	花粉壁形成 Pollen wall formation	脂肪酸羟化酶 Fatty acid hydroxylase	［25］
DPW	3	花药及花粉外壁的合成 Synthesis of anther and pollen outer wall	脂酰基载体蛋白还原酶 Fatty acyl carrier protein reductase	［26］
OsTDF1	3	绒毡层降解 Tapetum degeneration	R2R3 MYB转录因子 R2R3 MYB transcription factor	［27］
OsDEX1	3	绒毡层降解、花粉壁发育 Tapetum degeneration and pollen wall development	钙离子结合蛋白 Calcium-binding protein	［28］
OsDGD2β	3	花粉外壁发育 Pollen outer wall development	双内酯甘油合酶 Dialactone glycerol synthase	［29］
TIP3	3	调控乌氏体和花粉外壁形成 Regulation of Ubisch bodies and pollen wall formation	PHD指蛋白 PHD finger protein	［30］
EAT1	4	绒毡层降解 Tapetum degeneration	bHLH转录因子 bHLH transcription factor	［31］
OsACOS12	4	孢粉素合成相关 Related to sporopollenin synthesis	酰基辅酶A合成酶 Acyl-CoA synthetase	［32］
OsABCG15	6	花粉外壁发育相关 Related to pollen outer wall development	ATP结合盒转运蛋白 ATP-binding cassette transporters	［33］
OsMADS16	6	花器官发育相关 Related to floral organ development	B类花同源异型转录因子 B-class floral homeotic transcription factor	［34］
MIL1	7	孢子母细胞减数分裂、花药壁细胞的分化 Meiosis of spore mother cells and differentiation of anther wall cells	CC型谷氧还蛋白 CC-type glutaredoxin	［35］

基因 Gene	染色体 Chromosome	功能 Function	编码蛋白 Encoding protein	参考文献 Reference
DTM1	7	绒毡层发育、性母细胞发育 Tapetum and meiocytes development	内质网膜蛋白 Reticulon	［36］
UDT1	7	绒毡层细胞分化 Tapetal cell differentiation	bHLH转录因子 bHLH transcription factor	［37］
DTC1	7	绒毡层降解 Tapetum degeneration	PCD调控因子 PCD regulatory factor	［38］
CYP703A3	8	花药表皮角质层、花粉外壁的发育 Development of anther cuticle and pollen outer wall	脂肪酸羟化酶 Fatty acid hydroxylase	［39］
PTC1	9	绒毡层降解、花粉壁发育 Tapetum degeneration and Pollen wall development	PHD锌指蛋白 PHD zinc finger protein	［40］
OsGEN1	9	小孢子发育 Microspore development	RAD2/XPG核酸酶 RAD2/XPG nuclease	［41］
WDA1	10	花粉壁蜡质形成 Waxy formation of pollen wall	碳裂合酶 Carbon lyase	［42］
OsABCG26	10	花药角质层和花粉外壁的形成 Formation of anther cuticle and pollen outer wall	ATP结合盒转运蛋白 ATP-binding cassette transporter	［43］
OsC6	11	乌氏体和花粉外壁发育 Ubisch bodies and pollen wall development	脂质转运蛋白 Lipid transfer protein	［44］
OsGPAT3	11	花药壁发育 Pollen wall development	甘油-3-磷酸酰基转移酶 Glycerol-3-phosphate acyltransferase	［45］

基因 Gene	染色体 Chromosome	功能 Function	编码蛋白 Encoding protein	参考文献 Reference
DTM1	7	绒毡层发育、性母细胞发育 Tapetum and meiocytes development	内质网膜蛋白 Reticulon	［36］
UDT1	7	绒毡层细胞分化 Tapetal cell differentiation	bHLH转录因子 bHLH transcription factor	［37］
DTC1	7	绒毡层降解 Tapetum degeneration	PCD调控因子 PCD regulatory factor	［38］
CYP703A3	8	花药表皮角质层、花粉外壁的发育 Development of anther cuticle and pollen outer wall	脂肪酸羟化酶 Fatty acid hydroxylase	［39］
PTC1	9	绒毡层降解、花粉壁发育 Tapetum degeneration and Pollen wall development	PHD锌指蛋白 PHD zinc finger protein	［40］
OsGEN1	9	小孢子发育 Microspore development	RAD2/XPG核酸酶 RAD2/XPG nuclease	［41］
WDA1	10	花粉壁蜡质形成 Waxy formation of pollen wall	碳裂合酶 Carbon lyase	［42］
OsABCG26	10	花药角质层和花粉外壁的形成 Formation of anther cuticle and pollen outer wall	ATP结合盒转运蛋白 ATP-binding cassette transporter	［43］
OsC6	11	乌氏体和花粉外壁发育 Ubisch bodies and pollen wall development	脂质转运蛋白 Lipid transfer protein	［44］
OsGPAT3	11	花药壁发育 Pollen wall development	甘油-3-磷酸酰基转移酶 Glycerol-3-phosphate acyltransferase	［45］

基因 Gene	染色体 Chromosome	来源 Resource	不育类型 Sterile type	编码蛋白 Encoding protein	参考文献 Reference
pms1	7	农垦58S Nongken 58S	长光敏不育 Photoperiod-sensitive male sterility under long-day condition	长链非编码RNA Long non-coding RNA	［48］
pms2	3	32001S	长光敏不育 Photoperiod-sensitive male sterility under long-day condition	未知 Unknown	［49］
pms3/ p/tms12-1	12	农垦58S/培矮64S Nongken 58S/Peiai 64S	长光敏不育 Photoperiod-sensitive male sterility under long-day condition	长链非编码RNA/小RNA Long non-coding RNA/small RNA	［50-51］
pms4	4	绵9S Mian 9S	长光敏不育 Photoperiod-sensitive male sterility under long-day condition	未知 Unknown	［52］
ptgms2-1	2	广占63S Guangzhan 63S	长光敏不育 Photoperiod-sensitive male sterility under long-day condition	未知 Unknown	［53］
rpms1	8	宜D1S Yi D1S	短光敏不育 Photoperiod-sensitive male sterility under short-day condition	未知 Unknown	［54］
rpms2	9	宜D1S Yi D1S	短光敏不育 Photoperiod-sensitive male sterility under short-day condition	未知 Unknown	［54］
CSA	1	csa突变体 csa mutant	短光敏不育 Photoperiod-sensitive male sterility under short-day condition	R2R3类MYB转录因子 R2R3 MYB transcription factor	［55］
tms2	7	Norin-PL12	高温敏不育 Thermo-sensitive male sterility under high temperature	鞘脂合成相关 Related with sphingolipid synthesis	［56］
tms3	6	IR32364TGMS	高温敏不育 Thermo-sensitive male sterility under high temperature	未知 Unknown	［57］
tms4	2	TGMS-VN1	高温敏不育 Thermo-sensitive male sterility under high temperature	未知 Unknown	［58］
tms5	2	安农S，株1S Annong S， Zhu 1S	高温敏不育 Thermo-sensitive male sterility under high temperature	RNA酶Z Rnase Z	［59］
tms3650	3	tms3650	高温敏不育 Thermo-sensitive male sterility under high temperature	未知 Unknown	［60］
tms6	5	Sokcho-MS	高温敏不育 Thermo-sensitive male sterility under high temperature	未知 Unknown	［61］
tms9-1	9	衡农S-1 Hengnong S-1	高温敏不育 Thermo-sensitive male sterility under high temperature	未知 Unknown	［62］
tms10	2	9522S	高温敏不育 Thermo-sensitive male sterility under high temperature	类受体激酶 Receptor-like kinase	［63］