Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (9): 157-165.DOI: 10.13304/j.nykjdb.2022.0264
• BIO-MANUFACTURING & RESOURCE AND ECOLOGY • Previous Articles Next Articles
Jingjuan GAO1(), Chenyu ZHU1, Yuqin KE2, Chaoyuan ZHENG1, Chunying LI3, Wenqing LI3()
Received:
2022-04-04
Accepted:
2022-06-10
Online:
2023-09-15
Published:
2023-09-28
Contact:
Wenqing LI
高静娟1(), 朱晨宇1, 柯玉琴2, 郑朝元1, 李春英3, 李文卿3()
通讯作者:
李文卿
作者简介:
高静娟 E-mail:gaojjhnly@163.com;
基金资助:
CLC Number:
Jingjuan GAO, Chenyu ZHU, Yuqin KE, Chaoyuan ZHENG, Chunying LI, Wenqing LI. Effects of Organic Fertilizer Application Period on Carbon and Nitrogen Metabolism in Flue-cured Tobacco Under Tobacco-Rice Rotation[J]. Journal of Agricultural Science and Technology, 2023, 25(9): 157-165.
高静娟, 朱晨宇, 柯玉琴, 郑朝元, 李春英, 李文卿. 烟稻轮作条件下有机肥施用时期对烤烟碳氮代谢的影响[J]. 中国农业科技导报, 2023, 25(9): 157-165.
处理 Treatment | pH | 有机质 Organic matter/% | 总氮Total N/% | 总磷 Total P/ (mg·g-1) | 总钾 Total K/% | 水解性氮 Hydrolysable nitrogen/ (mg·kg-1) | 有效磷 Available phosphorus /(mg·kg-1) | 速效钾Available potassium/(mg·kg-1) | |
---|---|---|---|---|---|---|---|---|---|
基础土壤 Original soil-fertility | 4.96 | 2.47 | 0.148 | 0.636 | 2.07 | 190.7 | 67.67 | 192.5 | |
栽烟前土壤 Soil before planting tobacco | T1 | 4.76 | 2.53 | 0.140 | 0.683 | 2.12 | 128.3 | 68.96 | 101.8 |
T2 | 4.79 | 2.51 | 0.138 | 0.639 | 2.07 | 116.5 | 66.32 | 115.8 | |
T3 | 4.71 | 2.50 | 0.137 | 0.627 | 2.01 | 115.8 | 56.83 | 111.8 | |
T4 | 4.75 | 2.40 | 0.134 | 0.684 | 2.03 | 126.2 | 48.26 | 109.5 |
Table 1 Status of soil fertility in test plot
处理 Treatment | pH | 有机质 Organic matter/% | 总氮Total N/% | 总磷 Total P/ (mg·g-1) | 总钾 Total K/% | 水解性氮 Hydrolysable nitrogen/ (mg·kg-1) | 有效磷 Available phosphorus /(mg·kg-1) | 速效钾Available potassium/(mg·kg-1) | |
---|---|---|---|---|---|---|---|---|---|
基础土壤 Original soil-fertility | 4.96 | 2.47 | 0.148 | 0.636 | 2.07 | 190.7 | 67.67 | 192.5 | |
栽烟前土壤 Soil before planting tobacco | T1 | 4.76 | 2.53 | 0.140 | 0.683 | 2.12 | 128.3 | 68.96 | 101.8 |
T2 | 4.79 | 2.51 | 0.138 | 0.639 | 2.07 | 116.5 | 66.32 | 115.8 | |
T3 | 4.71 | 2.50 | 0.137 | 0.627 | 2.01 | 115.8 | 56.83 | 111.8 | |
T4 | 4.75 | 2.40 | 0.134 | 0.684 | 2.03 | 126.2 | 48.26 | 109.5 |
处理 Treatment | 叶绿素a含量 Chlorophyll a /(mg·g-1 FW) | 叶绿素b含量 Chlorophyll b /(mg·g-1 FW) | 总叶绿素含量 Chlorophyll /(mg·g-1 FW) | 类胡萝卜素含量 Carotenoids /(mg·g-1 FW) |
---|---|---|---|---|
T1 | 0.505±0.003 c | 0.172±0.019 b | 0.677±0.022 c | 0.165±0.001 d |
T2 | 0.664±0.026 b | 0.193±0.012 ab | 0.856±0.037 b | 0.218±0.008 b |
T3 | 0.601±0.013 b | 0.176±0.005 b | 0.777±0.018 b | 0.191±0.006 c |
T4 | 0.770±0.031 a | 0.214±0.005 a | 0.984±0.034 a | 0.252±0.012 a |
Table 2 Chloroplast pigment content of tobacco plant under different treatments
处理 Treatment | 叶绿素a含量 Chlorophyll a /(mg·g-1 FW) | 叶绿素b含量 Chlorophyll b /(mg·g-1 FW) | 总叶绿素含量 Chlorophyll /(mg·g-1 FW) | 类胡萝卜素含量 Carotenoids /(mg·g-1 FW) |
---|---|---|---|---|
T1 | 0.505±0.003 c | 0.172±0.019 b | 0.677±0.022 c | 0.165±0.001 d |
T2 | 0.664±0.026 b | 0.193±0.012 ab | 0.856±0.037 b | 0.218±0.008 b |
T3 | 0.601±0.013 b | 0.176±0.005 b | 0.777±0.018 b | 0.191±0.006 c |
T4 | 0.770±0.031 a | 0.214±0.005 a | 0.984±0.034 a | 0.252±0.012 a |
处理 Treatment | 净光合速率 Pn / (µmol·m-2·s-1) | 蒸腾速率Tr / (mmol·m-2·s-1) | 气孔导度Gs / (mmol·m-2·s-1) | 胞间二氧化碳浓度Ci /(µmol·mol-1) |
---|---|---|---|---|
T1 | 11.97±0.52 d | 2.19±0.14 b | 162.33±3.53 c | 228.33±3.53 a |
T2 | 17.53±0.76 b | 2.54±0.10 b | 238.67±6.84 a | 212.33±6.12 ab |
T3 | 14.60±0.26 c | 2.58±0.12 b | 197.00±8.75 b | 215.67±3.71 ab |
T4 | 19.87±0.62 a | 3.06±0.13 a | 250.33±5.78 a | 204.67±7.06 b |
Table 3 Photosynthetic characteristics of tobacco plant under different treatments
处理 Treatment | 净光合速率 Pn / (µmol·m-2·s-1) | 蒸腾速率Tr / (mmol·m-2·s-1) | 气孔导度Gs / (mmol·m-2·s-1) | 胞间二氧化碳浓度Ci /(µmol·mol-1) |
---|---|---|---|---|
T1 | 11.97±0.52 d | 2.19±0.14 b | 162.33±3.53 c | 228.33±3.53 a |
T2 | 17.53±0.76 b | 2.54±0.10 b | 238.67±6.84 a | 212.33±6.12 ab |
T3 | 14.60±0.26 c | 2.58±0.12 b | 197.00±8.75 b | 215.67±3.71 ab |
T4 | 19.87±0.62 a | 3.06±0.13 a | 250.33±5.78 a | 204.67±7.06 b |
处理 Treatment | 初始荧光 Fo | 最大荧光 Fm | 可变荧光 Fv | 最大光化学效率Fv/Fm | PS Ⅱ潜在光化学效率Fv/Fo |
---|---|---|---|---|---|
T1 | 4 911±54 b | 28 090±514 c | 23 585±314 b | 0.825±0.001 b | 4.719±0.044 c |
T2 | 4 958±128 b | 32 764±501 b | 24 916±613 ab | 0.847±0.005 a | 5.342±0.129 b |
T3 | 5 258±103 a | 33 932±1 085 ab | 24 641±712 ab | 0.852±0.005 a | 5.763±0.115 a |
T4 | 5 040±21 ab | 35 809±585 a | 25 426±309 a | 0.851±0.008 a | 5.774±0.087 a |
Table 4 Fluorescence characteristics of tobacco plant under different treatments
处理 Treatment | 初始荧光 Fo | 最大荧光 Fm | 可变荧光 Fv | 最大光化学效率Fv/Fm | PS Ⅱ潜在光化学效率Fv/Fo |
---|---|---|---|---|---|
T1 | 4 911±54 b | 28 090±514 c | 23 585±314 b | 0.825±0.001 b | 4.719±0.044 c |
T2 | 4 958±128 b | 32 764±501 b | 24 916±613 ab | 0.847±0.005 a | 5.342±0.129 b |
T3 | 5 258±103 a | 33 932±1 085 ab | 24 641±712 ab | 0.852±0.005 a | 5.763±0.115 a |
T4 | 5 040±21 ab | 35 809±585 a | 25 426±309 a | 0.851±0.008 a | 5.774±0.087 a |
Fig. 2 RuBPCase activity of flue-cured tobacco during harvest under different treatmentsNote: Different lowercase letters indicate significant differences between treatments at P<0.05 level.
Fig. 3 NR activity in flue-cured tobacco during harvest under different treatmentsNote: Different lowercase letters indicate significant differences between treatments at P<0.05 level.
Fig. 4 GS activity of flue-cured tobacco during harvest under different treatmentsNote: Different lowercase letters indicate significant differences between treatments at P<0.05 level.
Fig. 5 Activities of SPS and SS in tobacco plant under different treatmentsNote: Different lowercase letters indicate significant differences between treatments at P<0.05 level.
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