Phylogenic and Functional Profile of Ser/Thr Kinases in Synechocystis sp. PCC 6803

doi:10.13304/j.nykjdb.2021.0657

Abstract

Abstract:

Signal perception and transduction for organisms are tightly associated with their adaptability to environments. For signal transduction systems， two-component kinase systems or Ser/Thr kinase systems both in eukaryotes and prokaryotes have been studied and reviewed. As a prokaryotic photosynthetic model organism， except for the typical two-component His-Rep kinase systems， Synechocystis sp. PCC 6803 also possesses 12 genes encoding putative eukaryotic Ser/Thr kinases， which can be divided into 2 subfamilies according to their structural features， PKN2 subfamily and ABC1 subfamily. Genes encoding Ser/Thr kinases belonging to the PKN2 subfamily have been investigated mostly， while the rest belonging to the ABC1 subfamily mostly are functionally unknown. In this paper， the prediction of conserved domains and motifs， as well as protein cellular localizations of these 12 Ser/Thr kinases in Synechocystis sp. PCC 6803 was investigated. As the representative monocotyledon and dicotyledon， rice and Arabidopsis were chosen as reference objects， and by sequence analysis together with 15 rice ABC1Ks and 17 Arabidopsis ABC1Ks， the potential functions and evolutionary relationship of Ser/Thr kinases in Synechocystis sp. PCC 6803were discussed， which would provide new insights into a more comprehensive understanding of Ser/Thr kinase signaling process in Synechocystis sp. PCC 6803.

Key words: Synechocystis, signal transduction, Ser/Thr protein kinase, photosynthesis

摘要：

生物的信号感知与传导过程与生物对外界环境的适应性密不可分。提及信号传导系统，真核生物和原核生物中普遍存在的是双元激酶系统和丝氨酸/苏氨酸激酶系统，且关于这2种信号传导系统的研究已有诸多报道。作为一种典型的原核光合模式生物，集胞藻6803除了具有原核生物的双元信号传导系统之外，还具有由12个基因编码的真核类Ser/Thr激酶系统，根据它们的结构特点可以分为2大类，即PKN2亚家族和ABC1亚家族。编码属于PKN2亚家族的Ser/Thr激酶的基因大多数已有功能研究，而其他属于ABC1亚家族的基因大多数功能未知。对集胞藻6803中12个Ser/Thr蛋白激酶的保守域、基序以及蛋白细胞定位等进行分析，同时选取具有代表性的单子叶植物水稻和双子叶植物拟南芥作为参照对象，通过与水稻（15 ABC1Ks）和拟南芥（17 ABC1Ks）中的ABC1家族的蛋白激酶基因进行序列分析比较，从而对集胞藻6803中12个Ser/Thr蛋白激酶的潜在功能和进化关系进行了讨论分析，将为更全面地理解集胞藻6803中Ser/Thr蛋白激酶参与的信号传导过程提供新的见解和思路。

关键词: 集胞藻, 信号传导, 丝氨酸/苏氨酸蛋白激酶, 光合作用

CLC Number:

Xiaoting WANG, Pengpeng ZHANG. Phylogenic and Functional Profile of Ser/Thr Kinases in Synechocystis sp. PCC 6803[J]. Journal of Agricultural Science and Technology, 2023, 25(5): 66-76.

王小婷, 张芃芃. 集胞藻6803中丝氨酸/苏氨酸激酶系统发育和功能概述[J]. 中国农业科技导报, 2023, 25(5): 66-76.

Figures/Tables 7

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Gene product	Gene ID	Category
SpkA	sll 1574~1575	PKN2 subfamily
SpkB	slr 1697	PKN2 subfamily
SpkC	slr 0599	PKN2 subfamily
SpkD	sll 0776	PKN2 subfamily
SpkE	slr 1443	PKN2 subfamily
SpkF	slr 1225	PKN2 subfamily
SpkG	slr 0152	PKN2 subfamily
SpkH	sll 0005	ABC1 subfamily
SpkI	sll 1770	ABC1 subfamily
SpkJ	slr 0889	ABC1 subfamily
SpkK	slr 1919	ABC1 subfamily
SpkL	sll 0095	ABC1 subfamily

Gene product	Gene ID	Category
SpkA	sll 1574~1575	PKN2 subfamily
SpkB	slr 1697	PKN2 subfamily
SpkC	slr 0599	PKN2 subfamily
SpkD	sll 0776	PKN2 subfamily
SpkE	slr 1443	PKN2 subfamily
SpkF	slr 1225	PKN2 subfamily
SpkG	slr 0152	PKN2 subfamily
SpkH	sll 0005	ABC1 subfamily
SpkI	sll 1770	ABC1 subfamily
SpkJ	slr 0889	ABC1 subfamily
SpkK	slr 1919	ABC1 subfamily
SpkL	sll 0095	ABC1 subfamily

Gene	Function description
spkA	Involved in cell motility^{［31‑32］}
spkB	Participate in the oxidative stress response^［34］； Regulate cell motility^［30］
spkC	Participate in the regulation of nitrogen metabolism^［35］； Involved in the phosphorylation of the GroES chaperone protein^［38］
spkD	Involved in adjusting the pool of TCA cycle metabolites^［36］； Respond to limitation of inorganic carbon^［37］
spkE	Respond to cold stress^［38］； Involved in the regulation of nitrogen metabolism^［39］
spkF	Involved in the phosphorylation of the GroES chaperone protein^［38］
spkG	Involved in high-salt acclimation^［40］； Involved in the phosphorylation of Fd5^［41］
spkH	Involved in hyperosmotic stress response^［42］
spkK	Involved in the phosphorylation of the GroES chaperone protein^［38］

Gene	Function description
spkA	Involved in cell motility^{［31‑32］}
spkB	Participate in the oxidative stress response^［34］； Regulate cell motility^［30］
spkC	Participate in the regulation of nitrogen metabolism^［35］； Involved in the phosphorylation of the GroES chaperone protein^［38］
spkD	Involved in adjusting the pool of TCA cycle metabolites^［36］； Respond to limitation of inorganic carbon^［37］
spkE	Respond to cold stress^［38］； Involved in the regulation of nitrogen metabolism^［39］
spkF	Involved in the phosphorylation of the GroES chaperone protein^［38］
spkG	Involved in high-salt acclimation^［40］； Involved in the phosphorylation of Fd5^［41］
spkH	Involved in hyperosmotic stress response^［42］
spkK	Involved in the phosphorylation of the GroES chaperone protein^［38］

Gene product	Rice			*Arabidopsis*
Gene product	Homolog	Location	Predicted function	Homolog	Location	Predicted function
SpkH	OsABC1-4	Chloroplast	Cold stress^［48］	At3g24190	Chloroplast	Protein phosphorylation
SpkK	OsABC1-10	Mitochondria	Salt stress^［48］	At5g24970	Mitochondria	Protein phosphorylation
SpkI	OsABC1-12	Chloroplast	Cold and salt stress； biosynthesis of isoprenoids^{［48‑49］}	At3g07700 （AtSIA1； ABC1K7）	Chloroplast	Salt stress^［50］
SpkL	OsABC1-2	Chloroplast	Salt， H₂O₂ and darkness； energy metabolism^［49］	At5g64940 （AtOSA1； ABC1K8）	Chloroplast	Cadmium and oxidative stresses； isoprenyl lipid synthesis； distribution of iron within chloroplast^{［25， 51］}
SpkJ	OsABC1-14， -15	Vacuole	H₂O₂， cold， drought， and darkness^［48］	At1g65950 （ABC1K14）； At2g40090 （ABC1K15）	Extracellular region	Lipid homeostasis； protein phosphorylation