Journal of Agricultural Science and Technology ›› 2025, Vol. 27 ›› Issue (1): 147-154.DOI: 10.13304/j.nykjdb.2023.0546

• ANIMAL AND PLANT HEALTH • Previous Articles    

Symptoms and Pathogen Identification of Ficus carica Bacterial Leaf Spot Disease in Fujian

Mengting JI1(), Ling ZHU2, Xiaohua LUO3, Yuting ZHENG1, Shun XIAO1, Fangping HU1, Xueqing CAI1()   

  1. 1.College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2.Fujian Tianhe Lvbao Agricultural Materials Co. ,Ltd. ,Fuzhou 350199,China
    3.Agricultural and Rural Bureau of Lianjiang County,Fuzhou City,Fujian Lianjiang 350500,China
  • Received:2023-07-13 Accepted:2023-11-25 Online:2025-01-15 Published:2025-01-21
  • Contact: Xueqing CAI

福建无花果细菌性叶斑病的症状及病原菌鉴定

季梦婷1(), 朱玲2, 罗晓华3, 郑钰婷1, 肖顺1, 胡方平1, 蔡学清1()   

  1. 1.福建农林大学植物保护学院,福州 350002
    2.福建天禾绿宝农资有限公司,福州 350199
    3.福州市连江县农业农村局,福建 连江 350500
  • 通讯作者: 蔡学清
  • 作者简介:季梦婷 E-mail:1249169841@qq.com
  • 基金资助:
    国家重点研发计划项目(2017YFD0201106-02);福建省现代农业(水果)产业技术体系病虫害防控岗位项目(KKE19005A);福建省科技特派员工作经费项目(KTP21267A)

Abstract:

To clarify the taxonomy of the pathogen causing a new bacterial leaf spot disease on fig (Ficus carica) in Fuzhou in 2020, diseased fig leaves were collected as test materials. The bacterial strains were isolated from the diseased leaves by streak plate method, and then the pathogenicity of the strains was determined by tobacco allergic reaction, spray inoculated fig leaves and Koch’s rule. The pathogen was identified based on morphological characteristics, physiological and biochemical determination, and phylogenetic tree analysis using partial sequences of 16S rDNA, gyrB and rpoD. The results showed that 10 bacterial strains were isolated and purified from the diseased leaves, healthyfig leaves demonstrated similar symptoms as the specimen collected from fields, and the identical morphological bacterial strains were re-isolated from diseased fig leaves. Thus, all of the bacterial strains were identified as the causal pathogens of bacterial leaf spot disease by Koch’s rule. The colony morphology was light yellow, round, and flat on the NA medium after 2 d of culture, and the gram staining reaction was negative. The bacterial strains could produce green water-soluble fluorescent on KB medium. The sequencing analysis based on 16S rDNA, gyrB and rpoD showed that the test strains were clustered with Pseudomonas cichorii; and the results of physiological and biochemical characteristics, Biolog phenotypes instrument, and specific primers were similar to the P. cichorii strain 5707, therefore the 10 isolated bacterial strains were identified as P. cichorii. It was the first report that P. cichorii could naturally cause the leaf spot disease in F. carica.

Key words: Ficus carica bacterial leaf spot disease, pathogen identification, Pseudomonas chicorii, housekeeping gene

摘要:

为明确2020年以来在福州市郊区种植无花果上新发生的细菌性叶斑病的病因,在果园内采集病样进行病原菌分离纯化,利用烟草过敏性反应测定、喷雾接种无花果叶片以及柯赫氏法则验证,明确其致病性;通过对病原菌的形态特征观察、生理生化测定,结合16S rDNA、gyrBrpoD基因序列分析,确定病原菌的分类地位。结果表明,从病样中分离纯化获得10株细菌菌株;烟草过敏性反应阳性;人工接种结果显示,供试细菌可侵染健康的无花果叶片,并产生与田间相似的症状,且重新分离到与原菌落形态相同的细菌,柯赫氏法则证明这些菌株为该病害的病原菌。该病菌在NA培养基上培养2 d后,菌落为淡黄色、圆形、扁平;菌体形状为短杆状,革兰氏染色阴性;病菌在KB培养基上能产生绿色水溶性荧光色素;供试菌株的16S rDNA、gyrBrpoD基因序列分析结果显示,供试菌株与菊苣假单胞菌(Pseudomonas cichorii)聚为一支;其生理生化和Biolog表型测定结果以及菊苣假单胞菌特异性引物检测结果均与菊苣假单胞菌的模式菌株5707一致。因此将该致病菌鉴定为菊苣假单胞菌(P. cichorii)。这是首次发现菊苣假单胞菌在自然状态下侵染无花果并引起叶斑病。

关键词: 无花果细菌性叶斑病, 病原菌鉴定, 菊苣假单胞菌, 管家基因

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