Journal of Agricultural Science and Technology ›› 2025, Vol. 27 ›› Issue (1): 147-154.DOI: 10.13304/j.nykjdb.2023.0546
• ANIMAL AND PLANT HEALTH • Previous Articles
Mengting JI1(), Ling ZHU2, Xiaohua LUO3, Yuting ZHENG1, Shun XIAO1, Fangping HU1, Xueqing CAI1(
)
Received:
2023-07-13
Accepted:
2023-11-25
Online:
2025-01-15
Published:
2025-01-21
Contact:
Xueqing CAI
季梦婷1(), 朱玲2, 罗晓华3, 郑钰婷1, 肖顺1, 胡方平1, 蔡学清1(
)
通讯作者:
蔡学清
作者简介:
季梦婷 E-mail:1249169841@qq.com;
基金资助:
CLC Number:
Mengting JI, Ling ZHU, Xiaohua LUO, Yuting ZHENG, Shun XIAO, Fangping HU, Xueqing CAI. Symptoms and Pathogen Identification of Ficus carica Bacterial Leaf Spot Disease in Fujian[J]. Journal of Agricultural Science and Technology, 2025, 27(1): 147-154.
季梦婷, 朱玲, 罗晓华, 郑钰婷, 肖顺, 胡方平, 蔡学清. 福建无花果细菌性叶斑病的症状及病原菌鉴定[J]. 中国农业科技导报, 2025, 27(1): 147-154.
Fig. 1 Symptoms of bacterial leaf spot disease on Ficus carica in the fieldA: Symptom of whole plant; B: Early symptom; C: Middle symptom (yellow halo); D: Late symptom
Fig. 2 Front and back of fig leaf inoculated with bacterial strain W10 and uninoculated bacterial strainA: Front of leaf inoculated with W10; B: Back of leaf inoculated with W10; C: Front of leaf inoculated with water; D: Back of leaf inoculated with water
Fig. 3 Colony morphology characteristics of test strain on NA medium after culture 24 h and the flagellum under electron microscopyA: Plate colony; B: Single colony; C: Flagellum
Fig. 5 PCR amplification results of specific primer Hrp1a/Hrp2a of the test strainsNote:M—DL2000 marker;1~10—Strain W1~W10;11—Strain 5707; 12—strain Pc-GD-1.
项目Item | 供试菌株(W1~W10) Test strain(W1~W10) | 菌株 5707 | 项目Item | 供试菌株(W1~W10) Test strains(W1~W10) | 菌株 5707 |
---|---|---|---|---|---|
KB荧光 Produce fluorescence on KB | + | + | 丙二酸纳 sodium malonate | + | + |
革兰氏染色 Gram stain | - | - | 麦芽糖 Maltose | - | - |
精氨酸双水解 Arginine hydrolysis | - | - | 纤维二糖 Cellobiose | - | - |
过氧化氢反应 Reaction with hydrogen peroxide | + | + | 蔗糖 Sucrose | - | - |
氧化酶反应Oxidase test | + | + | 阿拉伯糖 L-arabinose | - | - |
硝酸钾还原反应 Reduction of potassium nitrate | - | - | 鼠李糖 L-rhamnose monohydrate | - | - |
吲哚的产生 Indole production | - | - | 海藻糖 D-trehalose anhydrous | - | - |
溶解果胶活性 Hydrolysis of pectinase | - | - | 棉籽糖 Raffinose | - | - |
果聚糖的产生 Fructan production | - | - | 山梨醇 Sorbitol | - | - |
淀粉水解 Starch hydrolysis | + | + | 酒石酸钠 Sodium tartaric | - | - |
甲基红试验 Methyl red test | - | - | 甜醇 Dulcitol | - | - |
V-P试验 V-P test | - | - | 丙三醇 Glycerol | + | + |
葡萄糖 Glucose | + | + | 香叶醇 Geraniol | - | - |
柠檬酸钠 Trisodium citrate dihydrate | - | - | 甘露醇 Mannitol | + | + |
Table 1 Results of physiological and biochemical characteristics of the test strains
项目Item | 供试菌株(W1~W10) Test strain(W1~W10) | 菌株 5707 | 项目Item | 供试菌株(W1~W10) Test strains(W1~W10) | 菌株 5707 |
---|---|---|---|---|---|
KB荧光 Produce fluorescence on KB | + | + | 丙二酸纳 sodium malonate | + | + |
革兰氏染色 Gram stain | - | - | 麦芽糖 Maltose | - | - |
精氨酸双水解 Arginine hydrolysis | - | - | 纤维二糖 Cellobiose | - | - |
过氧化氢反应 Reaction with hydrogen peroxide | + | + | 蔗糖 Sucrose | - | - |
氧化酶反应Oxidase test | + | + | 阿拉伯糖 L-arabinose | - | - |
硝酸钾还原反应 Reduction of potassium nitrate | - | - | 鼠李糖 L-rhamnose monohydrate | - | - |
吲哚的产生 Indole production | - | - | 海藻糖 D-trehalose anhydrous | - | - |
溶解果胶活性 Hydrolysis of pectinase | - | - | 棉籽糖 Raffinose | - | - |
果聚糖的产生 Fructan production | - | - | 山梨醇 Sorbitol | - | - |
淀粉水解 Starch hydrolysis | + | + | 酒石酸钠 Sodium tartaric | - | - |
甲基红试验 Methyl red test | - | - | 甜醇 Dulcitol | - | - |
V-P试验 V-P test | - | - | 丙三醇 Glycerol | + | + |
葡萄糖 Glucose | + | + | 香叶醇 Geraniol | - | - |
柠檬酸钠 Trisodium citrate dihydrate | - | - | 甘露醇 Mannitol | + | + |
菌株编号 Strain number | 最佳匹配种类 Best match species | 可能性 Probability | 相似性 Similarity | 位距值 Bit distance value |
---|---|---|---|---|
W1 | P. cichorii | 0.970 | 0.801 | 2.432 |
W2 | P. cichorii | 0.963 | 0.800 | 2.393 |
W3 | P. cichorii | 0.961 | 0.786 | 2.611 |
W5 | P. cichorii | 0.960 | 0793 | 2.394 |
W10 | P. cichorii | 0.907 | 0.721 | 2.969 |
Table 2 Biolog test results of the test bacterial strain
菌株编号 Strain number | 最佳匹配种类 Best match species | 可能性 Probability | 相似性 Similarity | 位距值 Bit distance value |
---|---|---|---|---|
W1 | P. cichorii | 0.970 | 0.801 | 2.432 |
W2 | P. cichorii | 0.963 | 0.800 | 2.393 |
W3 | P. cichorii | 0.961 | 0.786 | 2.611 |
W5 | P. cichorii | 0.960 | 0793 | 2.394 |
W10 | P. cichorii | 0.907 | 0.721 | 2.969 |
1 | 沈元月.我国无花果发展现状、问题及对策[J].中国园艺文摘,2018,34(2):75-78, 122. |
SHEN Y Y. Current situation, problems and countermeasures of fig development in China [J]. Chin. Hortic. Abstracts, 2018, 34(2):75-78, 122. | |
2 | 肖莹.无花果疫病病原鉴定及生物学特性研究[D].合肥:安徽农业大学,2020. |
XIAO Y. Identification and biological characteristics of the pathogen in phytophthora blight of fig [D]. Hefei: Anhui Agricultural University, 2020. | |
3 | 李丹丹.无花果炭疽病病原鉴定及生物学特性研究与有效药剂筛选[D].合肥:安徽农业大学,2019. |
LI D D. Identification and biological characteristics of the pathogen from fig anthracnose and screening of effective fungicides [D]. Hefei: Anhui Agricultural University, 2019. | |
4 | 王佳,程芳梅,张营营,等.无花果病虫害与自然灾害综合防控技术[J].安徽农学通报, 2020,26(23):86-88. |
WANG J, CHENG F M, ZHANG Y Y, et al.. Pests and natural disasters integrated control technology and prospect of figs [J]. Anhui Agric. Sci. Bull., 2020, 26(23):86-88. | |
5 | ŽARKO I, JOVANA B, IVAN N. Leaf spot disease on Philodendron scandens, Ficus carica and Actinidia deliciosa caused by Pseudomonas syringae pv. syringae in Serbia [J]. Eur. J. Plant Pathol., 2018, 151(4):1107-1113. |
6 | 麦合木提江·米吉提.中国无花果病毒的鉴定及其分子特征研究[D].北京:中国农业科学院,2015. |
Mijiti Maihemutijiang. Identification and molecular characterization of fig viruses in China [D]. Beijing: Chinese Academy of Agricultural Sciences, 2015. | |
7 | PREISING S, BORGES D F, DE QUEIROZ AMBRÓSIO M M, et al.. A fig deal: a global look at fig mosaic disease and its putative associates [J]. Plant Dis., 2021, 105(4):727-738. |
8 | 方中达.植病研究方法[M].北京:中国农业出版社, 1998:179-182,192-211. |
9 | COTTYN B, BAEYEN S, PAUWELYN E, et al.. Development of a real-time PCR assay for Pseudomonas cichorii, the causal agent of midrib rot in greenhouse-grown lettuce, and its detection in irrigating water [J]. Plant Pathol., 2011, 60(3):453-461. |
10 | LANE D J. 16S/23S rRNA Sequencing. In: STACKEBRANDTE, (eds)GOODFELLOW M. Nucleic Acid Techniques in Bacterial Systematics [M]. New York: Wiley, 1991:115-175. |
11 | SARA F S, DAVID S G. Evolution of the core genome of Pseudomonas syringae, a highly clonal, endemic plant pathogen [J]. Appl. Environ. Microbiol., 2004, 74(4):1999-2012. |
12 | 李宝聚.蔬菜主要病害2013年发生概况及2014年发生趋势[J].中国蔬菜, 2014(2):5-8. |
13 | 佘小漫,汤亚飞,何自福,等.番茄细菌性髓部坏死病病原的鉴定[J].植物病理学报,2017,47(2):180-186. |
SHE X M, TANG Y F, HE Z F, et al.. Identification of the pathogen of tomato bacterial pulp necrosis [J]. Acta Phytopathol. Sin., 2017, 47(2): 180-186. | |
14 | TIMILSINA S, ADKISON H, TESTEN A L, et al.. A novel phylogroup of Pseudomonas cichorii identified following an unusual disease outbreak on tomato [J]. Phytopathology, 2017, 107(11):1298-1304. |
15 | LAN D Y, SHU F L, LU Y H, et al.. First report of a leaf spot disease of tobacco caused by Pseudomonas cichorii in China [J/OL]. Plant Dis., 2022, 106(3):1058 [2023-06-30]. . |
16 | PATEL N, PATEL R, WYENANDT C A, et al.. First report of Pseudomonas cichorii causing bacterial leaf spot on Romaine Lettuce (Lactuca sativa var. longifolia) and Escarole (Cichorium endivia) in New Jersey [J/OL]. Plant Dis., 2021, 105(12):4150 [2023-06-30]. . |
17 | 钱鑫,谭志琼,邢梦玉,等.黄瓜菊苣假单胞菌叶斑病内生拮抗细菌的鉴定及促生作用[J].湖南农业大学学报(自然科学版), 2022,48(1):54-59. |
QIAN X, TAN Z Q, XING M Y, et al.. Isolation and screening of endophytic antagonistic bacteria against cucumber leaf spot Pseudomonas cichorii and research on its growth promotion effect [J]. J. Hunan Agric. Univ. (Nat. Sci.), 2022, 48(1):54-59. | |
18 | LI B J, LI H L, SHI Y X, et al.. First report of Pseudomonas cichorii causing leaf spot of vegetable sponge gourd in China [J]. Plant Dis., 2014, 98(1):153-162. |
19 | KITAZAWA Y, NETSU O, NIJO T, et al.. First report of bacterial leaf blight on cosmos (Cosmos bipinnatus Cav.) caused by Pseudomonas cichorii in Japan [J]. J. Gen. Plant Pathol., 2014, 80(6):499-503. |
20 | MARQUES E, BORGES R C, UESUGI C H. Identification and pathogenicity of Pseudomonas cichorii associated with a bacterial blight of gerbera in the Federal District [J]. Hortic. Bras., 2016, 34(2):244-248. |
21 | JANG Y W, YOON Y, MAHARJAN R, et al.. First report of Pseudomonas cichorii causing bacterial vein necrosis on perilla plants [Perilla frutescens (L.Britton).] in South Korea [J/OL]. Plant Dis., 2022, PMID:35700520 [2023-06-30]. . |
22 | WANG F, TANG T, MAO T, et al.... Pseudomonas cichorii causing leaf spot disease on Banxia (Pinellia ternata) in China [J/OL]. Plant Dis., 2022, 106(12): 35442052 [2023-06-30]. . |
23 | AHMAD T, LI G, WANG J, et al.. First report of bacterial leaf spot of Ficus benghalensis caused by Pseudomonas cichorii in Pakistan [J]. Plant Dis., 2022, PMID: 35536210 [2023-06-30]. . |
24 | WARD D W, WELLER R, BATESON M M. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community [J]. Nature, 1990, 345(6270):63-65. |
25 | CHELO M I, ZÉ-ZÉ L, TENREIRO R. Congruence of evolutionary relationships inside the Leuconostoc⁃Oenococcus⁃Weissella clade assessed by phylogenetic analysis of the 16S rRNA gene, dnaA, gyrB, rpoC and dnaK [J]. Int. J. Syst. Evol. Microbiol., 2007, 57:276-286. |
26 | 郝云婕,韩素贞. gyrB基因在细菌系统发育分析中的应用[J].生物技术通报,2008,56(2):39-41. |
HAO Y J, HAN S Z. Application of gyrB gene in bacterial phylogenetic analysis [J]. Biotech. Bull., 2008, 56(2):39-41. | |
27 | YAMAMOTO S, HARAYAMA S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains [J/OL]. Appl. Environ. Microbiol., 1995, 61(10):3768 [2023-06-30]. . |
28 | 张琦梦.伯克氏菌属植物病原菌DNA条形码检测技术研究[D].南京:南京农业大学,2016. |
ZHANG Q M. DNA Barcoding method for detection of plant pathogenic Burkholderia [D]. Nanjing: Nanjing Agricultural University, 2016. | |
29 | 韦小瑜,游旅,田克诚,等.4株气单胞菌临床分离株基于gyrB和rpoB基因序列的分子鉴定[J].应用预防医学,2016,22(5):386-389. |
WEI X Y, YOU L, TIAN K C, et al.. Molecular identification of 4 clinical Aeromonas isolates by gyrB and rpoB gene sequencing [J]. J. Appl. Prev. Med., 2016, 22(5):386-389. | |
30 | 卢佳琦,李市场,王大红,等.16S rDNA与gyrB序列联合法鉴定一株蜡样芽孢杆菌[J].河南科技大学学报(自然科学版),2018,39(1):73-77,83-89. |
LU J Q, LI S C, WANG D H, et al.. Combined 16S rDNA and gyrB gene sequence to identify a strain of Bacillus cereus [J]. J. Henan Univ. Sci. Technol. (Nat.. Sci.), 2018, 39(1): 73-77,83-89. | |
31 | YAMAMOTO S, KASAI H, ARNOLD D L, et al.. Phylogebny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequence of gyrB and rpoD genes [J]. Microbiology, 2000, 146:2385-2394. |
32 | MAEDA Y, SHINOHARA H, KIBA A, et al.. Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences [J]. Int. J. Syst. Evol. Microbiol., 2006, 56:1031-1038. |
33 | 马娜,齐琳,高晶晶,等. 5-ALA对高温下无花果扦插幼苗的生长及叶片叶绿素荧光特性的影响[J].南京农业大学学报,2015,38(4):546-553. |
MA N, QI L, GAO J J, et al.. Effects of 5-aminolevulinic acid on cutting growth under high temperature condition and leaf chlorophyll fast fluorescence characteristics of Ficus carica L. [J]. J. Nanjing Agric. Univ., 2015, 38(4):546-553. |
[1] | Jinlin LIU, Yingying CHEN, Xiaomei LAN, Haiyong CHEN, Pengsheng LI, Bi QIN, Ye YANG. Identification and Sensitivity to Fungicides of Pathogen Species of Hass Avocado Fruit Anthracnose [J]. Journal of Agricultural Science and Technology, 2025, 27(1): 138-146. |
[2] | Shifeng MU, Xiaolei WEN, Lina FENG, Dexuan ZHAO, Suhong GAO, Peng GAO, Huixia QI. Identification and Biological Characteristics of a Colletotrichum fructicola Causing Chestnut Internal Rot Disease [J]. Journal of Agricultural Science and Technology, 2024, 26(9): 122-128. |
[3] | Mengting JI, Changjiang CHEN, Liuhe LUO, Zhijian LIN, Menglin ZHAN, Bingye YANG, Fangping HU, Xueqing CAI. Pathogen Identification of Kiwi Bacterial Wilt in Fujian [J]. Journal of Agricultural Science and Technology, 2024, 26(4): 144-152. |
[4] | Pengsheng LI, Qingtai HUANG, Yongmei FAN, Meng WANG, Ye YANG. Identification of Pathogen Causing Bacterial Fruit Blotch of Melon in Hainan [J]. Journal of Agricultural Science and Technology, 2024, 26(3): 117-123. |
[5] | Yating WU, Xiaoyu WANG, Qingping TANG, Zun WU, Haofeng GAO, Xuexin HE, Zhanglei YANG, Xuying JIA, Peng SHAO. Isolation and Identification of Bacterial Etiology and Histopathological Study of Surface Ulcer of Cynoglossus semilaevis [J]. Journal of Agricultural Science and Technology, 2023, 25(6): 135-143. |
[6] | Yuping SHI, Yixian LIU, Guowei LI, Yi TANG, Liming DAI, Lanlan LI, Zhiying CAI. Isolation and Identification of Pathogen of Rubber Tree Phytophthora Leaf Fall Disease and Screening of Control Fungicides [J]. Journal of Agricultural Science and Technology, 2023, 25(11): 114-122. |
[7] | Keze YANG, Fang WU, Yujie WEI, Liangfang WANG, Hao CHANG, Zhitao WU, Xianzhong YANG. Pathogen Identification and Laboratory Drug Screening of Peony Root Rot in Cold and Cool Region of Hexi [J]. Journal of Agricultural Science and Technology, 2023, 25(10): 144-151. |
[8] | Jiahuan HUO, Xiaolei WEN, Shuangmin LI, Lina FENG, Shuhui LAN, Lixin DONG, Sirou GUO, Jianing LI, Jianhua WANG, Huixia QI. Identification and Biological Characteristics of the Pathogen Causing Root Rot of Atractylodes chinensis [J]. Journal of Agricultural Science and Technology, 2022, 24(5): 137-144. |
[9] | ZHANG Nana§, LI Shuangmin§, WEN Xiaolei, FENG Lina, WANG Junfeng, YANG Wenjie, HUO Jiahuan, LAN Shuhui, SUN Weiming, QI Huixia. Identification and Biological Characteristics of the Pathogen Causing Pink Disease of Chestnut [J]. Journal of Agricultural Science and Technology, 2021, 23(7): 145-152. |
[10] | WEN Xiaolei1,2, QI Huixia1*, SUN Weiming1, LIU Yijian1, FENG Lina1, MENG Tongyao2, HAN Zhiling1, CAO Jia1, WANG Junfeng1. Identification and Biological Characteristics of the Pathogen (Fusarium equiseti) Causing Shoot Blight of Atractylodes chinensis [J]. Journal of Agricultural Science and Technology, 2020, 22(5): 115-121. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||