Pathogen Identification and Laboratory Drug Screening of Peony Root Rot in Cold and Cool Region of Hexi

doi:10.13304/j.nykjdb.2023.0174

Abstract

Abstract:

In recent years， the root rot of Paeonia lactiflora occurred seriously in the cold and cool areas of Hexi， Gansu Province， causing some plants to die， which greatly affected the development of P. lactiflora industry. In order to identify the main pathogenic bacteria of peony root rot in cold and cool areas of Hexi， morphological characteristics and molecular biology methods were used to identify the pathogen and determine the pathogenicity of this strain. The results showed that the dominant strain SY2020-2 was pathogenic to P. lactiflora and was identified as Fusarium oxysporum based on morphological characteristics and hierarchical clustering. In order to screen the effective and low toxicity agents for the prevention and control of peony root rot， 10 kinds of agents were selected for virulence analysis. The results showed that 30% propionate had best effect for SY2020-2 with EC₅₀ 0.1 mg·L^-1， and the EC₅₀ of 43% fluorobacterium oxime lipids， 50% carbendazim， 10% phyllobacterazole， 20% fluxazole amide， 30% oxime bacteria amylazol were 1.2， 2.7， 3.5， 4.8 and 6.4 mg·L^-1， 25 g·L^-1 fluoxonitrile and 3% phenyloconazole showed poor toxicity.

Key words: peony root rot, pathogen identification, virulence assay

摘要：

近年来，芍药根腐病在甘肃河西冷凉地区发生严重，造成部分植株枯死，极大地影响了芍药产业的发展。为明确河西冷凉地区芍药根腐病的主要致病菌，采用形态学特征和分子生物学方法对该病的病原菌进行鉴定和致病性测定。结果表明，分离的优势菌SY2020-2对芍药具有致病性，根据形态学特征和分子生物学方法综合将其鉴定为尖孢镰孢菌（Fusarium oxysporum）。为筛选防治芍药根腐病的高效低毒药剂，选用10种药剂对SY2020-2进行室内毒力测定，结果表明，30%丙硫菌唑的毒力最强，EC₅₀为0.1 mg·L^-1；43%氟菌·肟菌脂、50%多菌灵、10%叶菌唑、20%氟唑菌酰胺和30%肟菌·戊唑醇的EC₅₀分别为1.2、2.7、3.5、4.8和6.4 mg·L^-1；25 g·L^-1咯菌腈和3%苯醚甲环唑的毒力相对较差。

关键词: 芍药根腐病, 病原鉴定, 毒力测定

CLC Number:

S682.1

Keze YANG, Fang WU, Yujie WEI, Liangfang WANG, Hao CHANG, Zhitao WU, Xianzhong YANG. Pathogen Identification and Laboratory Drug Screening of Peony Root Rot in Cold and Cool Region of Hexi[J]. Journal of Agricultural Science and Technology, 2023, 25(10): 144-151.

杨克泽, 吴芳, 魏玉杰, 汪亮芳, 常浩, 吴之涛, 杨宪忠. 河西冷凉地区芍药根腐病病原鉴定及室内药剂筛选[J]. 中国农业科技导报, 2023, 25(10): 144-151.

Figures/Tables 8

References 14

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药剂 Insecticide	剂量 Dosage/（mg·L^-1）	药剂 Insecticide	剂量 Dosage/（mg·L^-1）
30%丙硫菌唑 30% prothioconazole	0.50	25 g·L^-1咯菌腈 25 g·L^-1 fluoxonitrile	500.00
	0.25		250.00
	0.05		50.00
	0.03		10.00
	0.01		5.00
20%氟唑菌酰胺 20% fluxazole amide	50.00	30%肟菌·戊唑醇 30% oxime bacteria·amylazol	250.00
	25.00		50.00
	5.00		25.00
	2.50		5.00
	0.50		2.50
43%戊唑醇 43% pentazolol	50.00	3%苯醚甲环唑 3% phenyloconazole	5000.00
	25.00		2500.00
	5.00		500.00
	2.50		250.00
	0.50		50.00
43%氟菌·肟菌脂 43% fluorobacterium·oxime lipids	50.00	10%叶菌唑 10% phyllobacterazole	25.00
	25.00		5.00
	5.00		2.50
	2.50		0.50
	1.25		0.25
50%多菌灵 50% carbendazim	20.00	24%噻呋酰胺 24% tiafuramide	250.00
	4.00		50.00
	2.00		10.00
	0.40		5.00
	0.20		2.50

药剂 Insecticide	剂量 Dosage/（mg·L^-1）	药剂 Insecticide	剂量 Dosage/（mg·L^-1）
30%丙硫菌唑 30% prothioconazole	0.50	25 g·L^-1咯菌腈 25 g·L^-1 fluoxonitrile	500.00
	0.25		250.00
	0.05		50.00
	0.03		10.00
	0.01		5.00
20%氟唑菌酰胺 20% fluxazole amide	50.00	30%肟菌·戊唑醇 30% oxime bacteria·amylazol	250.00
	25.00		50.00
	5.00		25.00
	2.50		5.00
	0.50		2.50
43%戊唑醇 43% pentazolol	50.00	3%苯醚甲环唑 3% phenyloconazole	5000.00
	25.00		2500.00
	5.00		500.00
	2.50		250.00
	0.50		50.00
43%氟菌·肟菌脂 43% fluorobacterium·oxime lipids	50.00	10%叶菌唑 10% phyllobacterazole	25.00
	25.00		5.00
	5.00		2.50
	2.50		0.50
	1.25		0.25
50%多菌灵 50% carbendazim	20.00	24%噻呋酰胺 24% tiafuramide	250.00
	4.00		50.00
	2.00		10.00
	0.40		5.00
	0.20		2.50

药剂 Insecticide	剂量 Dosage/（mg·L^-1）	抑制率 Inhibition rate/%	毒力回归方程 Virulence regression equation	EC₅₀/（mg·L^-1）	相关系数Correlation coefficient
30%丙硫菌唑 30% prothioconazole	0.50	0.69	y = 0.469 2x + 6.943 7	0.1	0.979 7
	0.25	0.56
	0.05	0.48
	0.03	0.41
	0.01	0.30
20%氟唑菌酰胺 20% fluxazole amide	50.00	0.73	y = 0.629 9x + 6.459 5	4.8	0.961 5
	25.00	0.69
	5.00	0.56
	2.50	0.34
	0.50	0.29
43%戊唑醇 43% pentazolol	50.00	0.75	y = 0.644 7x+ 6.204 0	13.6	0.905 0
	25.00	0.47
	5.00	0.35
	2.50	0.27
	0.50	0.23
43%氟菌·肟菌脂 43% fluorobacterium· oxime lipids	50.00	0.89	y =0.770 9x+ 7.251 0	1.2	0.983 6
	25.00	0.84
	5.00	0.74
	2.50	0.59
	1.25	0.47
50%多菌灵 50 % carbendazim	20.00	0.80	y = 0.855 3x+ 7.150 4	2.7	0.953 4
	4.00	0.59
	2.00	0.32
	0.40	0.27
	0.20	0.20
25 g·L^-1咯菌腈 25 g·L^-1 fluoxonitrile	500.00	0.65	y = 0.551 0x+ 5.502 5	124.4	0.975 8
	250.00	0.53
	50.00	0.41
	10.00	0.32
	5.00	0.19
30%肟菌·戊唑醇 30% oxime bacteria·amylazol	250.00	0.95	y = 0.9611x+ 7.1078	6.4	0.989 9
	50.00	0.81
	25.00	0.65
	5.00	0.46
	2.50	0.38
3%苯醚甲环唑 3% phenyloconazole	5000.00	0.81	y = 0.901 7x+ 5.199 9	600.2	0.968 4
	2500.00	0.76
	500.00	0.38
	250.00	0.32
	50.00	0.22

药剂 Insecticide	剂量 Dosage/（mg·L^-1）	抑制率 Inhibition rate/%	毒力回归方程 Virulence regression equation	EC₅₀/（mg·L^-1）	相关系数Correlation coefficient
30%丙硫菌唑 30% prothioconazole	0.50	0.69	y = 0.469 2x + 6.943 7	0.1	0.979 7
	0.25	0.56
	0.05	0.48
	0.03	0.41
	0.01	0.30
20%氟唑菌酰胺 20% fluxazole amide	50.00	0.73	y = 0.629 9x + 6.459 5	4.8	0.961 5
	25.00	0.69
	5.00	0.56
	2.50	0.34
	0.50	0.29
43%戊唑醇 43% pentazolol	50.00	0.75	y = 0.644 7x+ 6.204 0	13.6	0.905 0
	25.00	0.47
	5.00	0.35
	2.50	0.27
	0.50	0.23
43%氟菌·肟菌脂 43% fluorobacterium· oxime lipids	50.00	0.89	y =0.770 9x+ 7.251 0	1.2	0.983 6
	25.00	0.84
	5.00	0.74
	2.50	0.59
	1.25	0.47
50%多菌灵 50 % carbendazim	20.00	0.80	y = 0.855 3x+ 7.150 4	2.7	0.953 4
	4.00	0.59
	2.00	0.32
	0.40	0.27
	0.20	0.20
25 g·L^-1咯菌腈 25 g·L^-1 fluoxonitrile	500.00	0.65	y = 0.551 0x+ 5.502 5	124.4	0.975 8
	250.00	0.53
	50.00	0.41
	10.00	0.32
	5.00	0.19
30%肟菌·戊唑醇 30% oxime bacteria·amylazol	250.00	0.95	y = 0.9611x+ 7.1078	6.4	0.989 9
	50.00	0.81
	25.00	0.65
	5.00	0.46
	2.50	0.38
3%苯醚甲环唑 3% phenyloconazole	5000.00	0.81	y = 0.901 7x+ 5.199 9	600.2	0.968 4
	2500.00	0.76
	500.00	0.38
	250.00	0.32
	50.00	0.22

药剂 Insecticide	剂量 Dosage/（mg·L^-1）	抑制率 Inhibition rate/%	毒力回归方程 Virulence regression equation	EC₅₀/（mg·L^-1）	相关系数Correlation coefficient
10%叶菌唑 10% phyllobacterazole	25.00	0.68	y = 0.533 5x+6.311 9	3.5	0.971 0
	5.00	0.47
	2.50	0.41
	0.50	0.35
	0.25	0.21
24%噻呋酰胺 24% tiafuramide	250.00	0.76	y = 0.618 8x+6.098 9	16.8	0.993 5
	50.00	0.62
	10.00	0.46
	5.00	0.40
	2.50	0.28