Effects of Bacillus thuringiensi on Intestinal Bacteria in Ectropis obliqua

doi:10.13304/j.nykjdb.2023.0794

Abstract

Abstract:

Ectropis obliqua is one of the most important pests on tea， which has brought serious economic losses to the tea industry. Bacillus thuringiensis （Bt）?， as a key biological insecticide for the control of E. obliqua， plays an important role in the green prevention and control of diseases and pests in tea gardens. To clarify the effect of Bttreatment on intestinal bacteria of E. obliqua larvae， the Illumina platform using 16S rDNA high-throughput sequencing was applied to compare the structure and population diversity of intestinal commensal community in E. obliqua larvae treated with different dosage of Bt suspensions. The results showed that， compared with CK treatment， the intestinal bacterial composition of E. obliqua larvae treated with lower dosage of Bt did not change significantly， but those treated with higher dosage of Bt had significant effect on the composition of intestinal bacteria in E. obliqua larvae. And the treatments with higher dosage of Bt significantly increased the diversity and abundance of intestinal bacteria in E. obliqua larvae， which indicated that intestinal microorganisms were involved in the response of E. obliqua larvae to Bt infection. Above results provided basis for further exploring the insecticidal mechanism of Bt， and provided new ideas for improving the virulence of Bt.

Key words: Ectropis obliqua, Bacillus thuringiensis, biological control, intestinal bacteria, 16S rDNA

摘要：

茶尺蠖（Ectropis obliqua）是茶叶上的重要害虫，给茶叶产业带来严重经济损失。苏云金芽孢杆菌（Bacillus thuringiensis， Bt）作为防治茶尺蠖的关键生物杀虫剂，在茶尺蠖的绿色防控中发挥重要作用。为明确Bt处理对茶尺蠖幼虫肠道菌群的影响，基于Illumina平台，采用16S rDNA高通量测序技术对不同水平Bt悬浮液处理的茶尺蠖幼虫肠道细菌的群落结构和种群多样性进行比较分析。结果表明，使用低剂量Bt处理茶尺蠖幼虫后，其肠道细菌组成与对照处理相比并未发生明显变化；但在较高剂量Bt处理下，茶尺蠖幼虫肠道细菌的群落多样性及丰度显著增加，表明茶尺蠖幼虫肠道细菌群落参与了对Bt侵染的响应。以上研究结果为深入探究Bt杀虫机制提供依据，并为提高Bt杀虫毒力提供新的思路。

关键词: 茶尺蠖, 苏云金芽孢杆菌, 生物防治, 肠道细菌, 16S rDNA

CLC Number:

S476

Ruoheng JIN, Xiaoyu LI, Jingwu YAO, Beibei WANG, Chunxia CAO, Daye HUANG. Effects of Bacillus thuringiensi on Intestinal Bacteria in Ectropis obliqua[J]. Journal of Agricultural Science and Technology, 2025, 27(2): 141-149.

金若珩, 李晓宇, 姚经武, 王蓓蓓, 曹春霞, 黄大野. 苏云金芽孢杆菌对茶尺蠖肠道细菌多样性的影响[J]. 中国农业科技导报, 2025, 27(2): 141-149.

Figures/Tables 7

Fig. 1 Rarefaction curve of intestinal bacteria in E. oblique larvae under different treatment

Table 1 Composition of intestinal bacteria in E. oblique larvae under different Bt treatments

处理 Treatment	OTU数量 Number of OTU	门 Phylum	纲 Class	目 Order	科 Family	属 Genus	种 Species
CK	153	6.33	7.67	18.00	25.33	39.33	8.00
Bt50	200	8.00	10.33	21.33	27.67	41.00	10.67
Bt100	137	6.67	8.33	17.67	24.00	32.33	11.00
Bt200	125	5.67	7.00	16.33	22.00	27.00	11.00

Fig. 2 OTUs distribution of intestinal bacteria in E. oblique larvae under different Bt treatments

Fig. 3 Alpha diversity of intestinal bacteria in of E. oblique larvae under different treatmentsNote： * indicates significant at P<0.05 level.

Fig. 4 NMDS analysis of intestinal bacteria in of E. oblique larvae under different treatments

Fig. 5 Composition and abundance of intestinal bacterial populations in E. oblique under different treatmentsA~B： Phylum level； C~D： Genus level

Fig. 6 LEfSe analysis of intestinal bacterial populations in E. oblique under different treatments

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