Abstract:

Pyrosequencing of 16S rDNA genes was used to analyze and compare the differences of intestinal bacteria communities of Bama miniature pigs (BAMA), Black Xiang pigs (BLACK) and Yorkshire-Landrace-Duroc pigs (CROSS). The results showed that the diversity and abundance of intestinal bacteria varied among BAMA,BLACK and CROSS. Weeksella, Comamonas and Mitsuokella genus containing pathogenic bacteria were only detected in BAMA intestines, but they were not detected in BLACK and CROSS intestines. The diversity and abundance of Fibrobacter and Ruminococcus genus containing cellulose decomposing bacteria were higher in BAMA intestines than those in BLACK and CROSS intestines. The high content of pathogens and cellulose degrading bacteria in the intestines of BAMA suggested the better tolerance of BAMA to harsh conditions and high resistance against disease. BAMA was equipped for high roughage-resistance and liable to feeding management.

Key words: pig breeds, intestinal bacteria community, 454 pyrosequencing, 16S rDNA

摘要：

454 焦磷酸测序用于分析比较巴马香猪(BAMA)、黑香猪(BLACK)与外三元猪(CROSS)的肠道菌群差异。结果表明：三个猪种的肠道细菌多样性与丰度各有不同,差异较大。威克氏菌属、丛毛单胞菌属与光岗菌属等病原菌在BAMA肠道内检测到,而在BLACK与CROSS肠道内未检测到。BAMA肠道中丝状杆菌属与瘤胃球菌属等纤维素分解菌的多样性与丰度远大于在BLACK与CROSS肠道中的分布量。BAMA肠道内高含量的潜在病原菌与纤维分解菌表明BAMA对恶劣环境的适应性与抗病力较强,以及耐粗饲性强,容易饲养管理。

关键词: 猪种, 肠道菌群, 454 焦磷酸测序, 16S rDNA