Abstract:

Lignin is a common refractory material in the nature, which was abundant and complex, and concealed in crop straw and municipal solid waste. Some bacterial has the function of degrading lignin. This paper took deadwood and dead leaves as the sample, screened C\|9 strain by aniline blue plate method, which can degrade lignin and has a higher ability to produce enzymes, so as to attain the high efficient bacteria of lignin\|degrading, and provide reference for the study on lignin\|degrading by bacterial. It was identified as Arthrobacter sp. through microbial morphology and 16SrDNA. The optimization of enzyme\|producing condition for C\|9 strain was confirmed by the optimization of enzyme\|producing conditions: temperature 30～35℃, rotate speed 150 r/min, culture basis of pH 7～8, the optimal carbon source was starch, the optimal nitrogen source was peptone, and the amount of MnSO4 inducer was 0.8 mmol/L. Under the optimal condition, the lignin peroxidase yield of C\|9 strain was 74.62 U/mL, the manganese peroxidase yield of C\|9 strain was 58.61 U/mL, and the laccase activity was 68.92 U/mL.

Key words: lignin, lignin\, degrading bacteria, lignin\, degrading enzymes, condition optimization

摘要：

木质素是自然界中含量丰富、结构复杂，是农作物秸秆以及城市生活垃圾中一种常见的难降解物质，一些细菌具有降解木质素的功能。以朽木和枯叶作为样品，采用苯胺蓝平板法筛选出1株能够降解木质素且产酶能力较高的菌株C\|9，已期获得高效降解木质素的细菌，对细菌降解木质素体系提供参考。经微生物形态学和16SrDNA鉴定为节杆菌属（Arthrobacter sp.）；经产酶条件优化，确定菌株C\|9的产酶最优条件为：温度30～35℃，转速150 r/min，培养液的初始pH 7～8之间，最佳碳源为淀粉，最佳氮源为蛋白胨，诱导剂MnSO4浓度0.8 mmol/L；在最优条件下，菌株C\|9的木质素过氧化物酶活性74.62 U/mL，锰过氧化物酶活性58.61 U/mL,漆酶活性为68.92 U/mL。

关键词: 木质素；木质素降解菌；木质素降解酶；条件优化