Abstract: In order to identify the immunogenicity of porcine parvovirus VP2 fused with porcine interferon γ(IFN-γ), VP2 of PPV was fused with IFN-γ via overlapping extension polymerase chain reaction technique, and rBac-VP2, rBac-VP2-IFN-γ were constructed by baculoviruses expression vector system. The immunogenicity of the recombinant baculoviruses expressing VP2 of PPV fused with or without IFN-γ (rBac-VP2-IFN-γ and rBac-VP2) was characterized in BALB/c mice, and set the inactivated PPV vaccine as the control. The level of ELISA antibodies against VP2 and neutralizing antibodies against PPV, and the magnitude of lymphocyte proliferation from the mice immunized with rBac-VP2-IFN-γ vaccine were significantly higher than those of mice shot with rBac-VP2 vaccine or inactivated PPV vaccine. Together, this study showed that IFN-γ could improve the cellular and humoral immune response of subunit vaccine of PPV. This result has provided theoretical basis for the development of PPV subunit vaccine with porcine IFN-γ.

Key words: porcine parvovirus, VP2, IFN-γ, recombinant baculovirus, immunogenicity

摘要： 为了检验细胞因子IFN-γ与猪细小病毒VP2融合蛋白的免疫效力，通过重叠延伸PCR技术将IFN-γ与PPV的VP2基因融合到一起，利用杆状病毒表达系统构建了重组杆状病毒rBac-VP2、rBac-VP2-IFN-γ，并在小鼠上验证了免疫原性。猪细小病毒ELISA抗体和中和抗体检测结果显示，VP2-IFN-γ组明显高于VP2和PPV灭活苗组；淋巴细胞增殖试验结果表明，VP2-IFN-γ组淋巴细胞数明显高于不加IFN-γ组。上述结果表明IFN-γ可提高猪细小病毒亚单位疫苗体液和细胞免疫应答，为重组IFN-γ的猪细小病毒病亚单位疫苗开发提供理论依据。

关键词: 猪细小病毒；VP2；IFN-&gamma, ；重组杆状病毒；免疫原性