Abstract: Selenium (Se) and sulfur (S) are two important nutrients for flue-cured tobacco. Rational combined of Se and S has an important role in improving quality of flue-cured tobacco. This paper seeked proper combination of Se and S to provide basis for field fertilization. Taking flue-cured tobacco ‘Yuyan 10’ as material, this paper studied  the effect of different combination of Se and S concentration on Se and S uptake accumulation of tobacco plant and related metabolism enzymes by pot experiment. The results showed that ① Se content and accumulation in tobacco plant increased with Se applying level increase. While increase S level could restrain S uptake and accumulation in tobacco plant. ② Se application promoted S accumulation. At low S level, S content of tobacco leaf was increased by low concentration of Se, and high concentration of Se had no considerably effect on the S content of tobacco leaf. At high S level, Se application was helpful for increasing S content of tobacco leaf. ③ Under same Se application level, compared with low S level, APR activity of tobacco leaf at early stage was depressed under high S level, SAT activity increased and enzyme activity decreased, and SMT activity declined slightly at late stage. Under the same S application level, Se application treatment was helpful to increase SAT and SMT activity of tobacco leaf, but had no influence on APR activity. Generally, S1Se2 treatment was optimum, because higher Se content and lower S content was good for tobacco leaf.

Key words: flue-cured tobacco, application of sulfur and selenium combinatio, uptake and accumulation, metabolism enzyme

摘要： 硒、硫元素是烤烟重要的营养物质，合理的硒、硫配施对烤烟的品质改善起着重要的作用。探寻合适的硒、硫配施组合能为大田施肥提供依据。以豫烟10号为材料，采用盆栽试验研究不同浓度的硒硫配施对烟株硒、硫元素吸收积累及相关代谢酶的影响。结果显示：①烟株各器官硒含量和积累量随着施硒水平增加而增加，而提高硫水平则抑制烟株对硒元素的吸收积累。②施硒处理促进烟株硫元素的积累。其中在低硫水平时，低浓度硒能显著提高烟叶硫含量，高浓度硒对烟叶硫含量无显著影响；在高硫水平时，施硒处理均有利于提高烟叶的硫含量。③在相同的施硒水平下，与低硫水平相比较，高硫水平下的叶片APR酶活性在生长前期被抑制，SAT酶活性升高且酶活性下降时间延迟，SMT酶活性在生长后期有降低的趋势。在相同的施硫水平下，施硒处理有利于提高叶片SAT和SMT酶活，而APR酶活则变化不大。总的看来，低硫高硒处理下烟叶硒含量最高且硫含量较低，尤以S1Se2处理最为适宜。

关键词: 烤烟, 硒硫配施, 吸收积累, 代谢酶