Abstract: This paper studied the carbon and nitrogen metabolism, chemical components and ultrastructures of flue-cured tobacco under effects of three treatments, i. e. artificial excepting the bud (T1), pouring flumetralin 1 time by cup(T2), pouring flumetralin 2 times by cup(T3). The results showed：①The activities of amylase, invertase and sucrose synthase of T2 were significantly higher than T1 at 21 d and 28 d after topping. ②At 14 d, 21 d and 28 d after topping, the activity of NR of T2 was significantly higher than T1 and T3; the activity of GS of T2 was significantly higher than T3; T2 and T1 had no significant difference in GDH activity, but both were significantly higher than T3. ③The contents of total sugar and reducing sugar were T2>T1>T3; the content of starch was T2>T3>T1; at 14 d, 21 d and 28 d after topping, the content of total nitrogen of T2 was significantly higher than T1; the contents of nicotine of T2 and T3 were significantly lower than T1. ④The leaf of artificial inhibition bud developed normally, and the ultrastructure of the leaves of pouring prime was destroyed to some extent. The carbon and nitrogen metabolism was enhanced and coordinated, and leaf development was more reasonable for pouring prime 1 time by cup.

Key words: flumetralin, flue-cured tobacco, carbon and nitrogen metabolism, enzyme activity, leaf ultrastructure

摘要： 研究了人工抑芽（T1）、杯淋氟节胺1次（T2）、杯淋氟节胺2次（T3）3种农艺措施对烤烟碳氮代谢关键酶、化学成分以及叶片超微结构的影响。结果表明：①打顶后21 d和28 d，T2的淀粉酶、转化酶和蔗糖合成酶活性均显著高于T1。②打顶后14 d、21 d和28 d，T2的NR活性显著高于T1和T3，T2的GS活性显著高于T3，T2和T1的GDH活性无显著差异但均显著高于T3。③总糖和还原糖含量表现为T2>T1>T3，淀粉含量表现为T2>T3>T1；打顶后14 d、21 d和28 d，T2的总氮含量显著高于T1，T2和T3的烟碱含量均显著低于T1。④人工抑芽叶片发育正常，施用氟节胺会在一定程度上破坏叶片超微结构。氟节胺杯淋1次的烤烟叶片碳氮代谢旺盛，协调性更好，叶片发育较合理。

关键词: 氟节胺, 烤烟, 碳氮代谢, 酶活性, 叶片超微结构