Abstract: In order to explore the effects of soil conditioner on soil microbial functional diversity in tobacco planting fields, this study took flue-cured tobacco variety Yunyan 87 as material to explore the differences of microbial carbon metabolism fingerprints in tobacco planting soil by Biolog-Eco detection method under 4 different levels of soil conditioner including CK(0 kg/hm2), T1(1 500 kg/hm2), T2(3 000 kg/hm2), T3（4 500 kg/hm2）. The results showed that: ① When the application amount of soil conditioner was 3 000 kg/hm2, flue-cured tobacco had the strongest ability to use the 4 kinds of carbon sources, including carboxylic acids, amines, polymers and other types during tobacco maturing period. ② Principal component analysis showed that there were 4 kinds of sensitive carbon sources to distinguish soil conditioner, including carbohydrate, carboxylic acids, amino acids and amines; while sensitive carbon sources polymers and other types were to distinguish soil conditioner application amount. ③ Soil microbial community functional diversity index showed that T2 had the highest of mcIntosh index, 82.85% higher than that of the CK. Moreover, there were no significant difference in Richness index and Simpson index among the 4 treatments. Thus, it could be seen that the application of soil conditioner had significantly improved carbon metabolism intensity and community functional diversity of the tobacco planting soil. 3 000 kg/hm2 application rate had the best performance. The above results provided certain theoretical basis for applying soil conditioner in tobacco planting fields.

Key words: Biolog-ECO, soil conditioner, carbon metabolism fingerprint, tobacco planting soil

摘要： 为探究土壤调理剂对植烟土壤微生物功能多样性的影响，以烤烟品种云烟87为材料，采用Biolog-ECO检测法探究了土壤调理剂在CK（0 kg/hm2）、T1（1 500 kg/hm2）、T2（3 000 kg/hm2）、T3（4 500 kg/hm2）四种不同施用水平下植烟土壤微生物碳代谢指纹的差异。结果表明：①当土壤调理剂的施用量为3 000 kg/hm2时，烤烟成熟期羧酸类、胺类、聚合物类、其他类这4类碳源的利用能力最强。②主成分分析结果显示，糖类、羧酸类、氨基酸类、胺类这4类碳源是区分施用土壤调理剂的敏感碳源，而聚合物类和其他类碳源是区分土壤调理剂施用量的敏感碳源。③土壤微生物群落功能多样性指数结果显示，T2处理的均一性指数最高，较对照提高了82.85%，而各处理间的丰富度指数与优势度指数差异不显著。由此可见，施用土壤调理剂可显著提高植烟土壤微生物碳代谢强度、群落功能多样性，3 000 kg/hm2的施用量表现最优。上述结果为植烟土壤中土壤调理剂的施用提供一定的理论依据。

关键词: Biolog-ECO, 土壤调理剂, 碳代谢指纹, 植烟土壤