Abstract: In order to analyze the function of gene VdTri15 in Verticillium dahliae Kleb, this study deleted VdTri15 gene from Vd991, a virulent and defoliating strain of V. dahliae, by gene replacement strategy based on gene homologous recombination. Taking the virulent defoliating V. dahliae isolate Vd991 as the contrast, this paper compared the differences between deleted mutant and wild type in growth rate, excrine toxin and pathogenicity etc.; and discussed the function of VdTri15 gene in pathogenic process. The results indicated that VdTri15 mutation did not affect germ growth, but could weaken its virulence and change the type of toxins. This implied VdTri15 gene affect V.dahliae by influencing the synthesis of related toxins.

Key words: cotton verticillium wilt, Verticillium dahliae, gene knockout, VdTri15 gene

摘要： 为分析棉花黄萎病菌VdTri15基因的功能，利用基因同源重组原理将强致病力落叶型大丽轮枝菌Vd991菌株的VdTri15基因进行敲除。以Vd991菌株为对照，比较分析敲除突变体与野生型之间在生长速度、外分泌毒素、致病性等方面的差异，探讨VdTri15基因在致病过程中的功能。结果表明，VdTri15突变并不影响病菌的生长，但可引起其致病力减弱，分泌毒素种类发生改变。由此说明，VdTri15基因通过影响相关毒素的合成来影响大丽轮枝菌的致病力。

关键词: 棉花黄萎病, 大丽轮枝菌, 基因敲除, VdTri15基因