Abstract: Clostridium perfringens is a common pathogen in human, livestock and poultry, which can cause acute food poisoning, chronic intestinal infection in human or necrotizing enteritis in animals. It seriously endangers human health and the development of livestock and poultry breeding. In this paper, the effects of the specific carbon sources such as epilactose and its isomers lactose and lactulose were studied by the single-strain culture of Bifidobacterium longum and the co-culture of B. longum and C. perfringens. The results showed that B. longum could grow well in mTPY medium with epilactose as a carbon source and showed a similar growth trend as lactose. In addition, B. longum could significantly inhibit the growth of C. perfringens in a co-culture system, and the viability of B. longum in the epilactose medium was significantly better than that in lactose and lactulose ones. Moreover, an oxford cup test was also applied to measure the antibacterial effect of B. longum on C. perfringens, showing that the culture supernatant of B. longum had a good antibacterial activity against C. perfringens. These results provided theoretical reference for the inhibition of C. perfringens by B. longum, and also provide important guidance for further study of epilactose and its regulation mechanism.

Key words: Clostridium perfringens, Bifidobacterium longum, epilactose, co-culture, bacteriostatics

摘要： 产气荚膜梭菌是一种常见的人畜禽共患的病原菌，可引起人的急性食物中毒和慢性肠道感染，或导致动物的坏死性肠炎等，严重危害人体健康和畜禽养殖业的发展。采用对长双歧杆菌和产气荚膜梭菌单独培养和共培养的方式，研究表乳糖及其同分异构体乳糖、乳果糖等特异性碳源对双歧杆菌的生长特性及其抑制产气荚膜梭菌生长的影响。结果表明，长双歧杆菌能够在以表乳糖为碳源的mTPY培养基中生长良好，并显现出与乳糖相似的生长趋势。在与产气荚膜梭菌共培养的过程中，长双歧杆菌能够显著地抑制产气荚膜梭菌的生长，且表乳糖相较于乳糖和乳果糖，能显著提高长双岐杆菌的存活率。还利用牛津杯法对长双歧杆菌培养上清液对产气荚膜梭菌的抑菌效果进行评价，证明其培养上清液对于产气荚膜梭菌具有较好的抑菌活性。上述结果为应用长双歧杆菌抑制产气荚膜梭菌提供理论依据，对深入研究表乳糖调控机制具有重要的指导意义。

关键词: 产气荚膜梭菌, 长双歧杆菌, 表乳糖, 共培养, 抑菌