Abstract: Chestnut rot is the main disease caused serious loss to the chestnut industry during storage. Penicillium spinulosum is one of the main pathogens causing the disease, so the culture conditions were studied and 6 fungicides were screened through mycelial growth method to effectively control it.The results showed that the best mycelial growth medium was chestnut for Penicillium spinulosum with colony 3.72 cm. The PDA medium was most suitable for sporulation, and the amount of sporulation was 2.40×108 cell·L-1. The optimum nitrogen source was yeast extract for mycelial growth and sporulation, and the mycelial growth and sporulation were 3.42 cm and 2.93×108 cell·L-1, respectively. The optimum carbon source for mycelia growth was soluble starch, the colony diameter was 3.35 cm, and the optimum carbon source for sporulation was mannitol, the spore amount was 1.97×108 cell·L-1. The darkness was most suitable for mycelial growth, the colony diameter was 4.35 cm. Sporulation was not strict with lighting condition. The suitable temperature for mycelial growth was 20 ℃; suitable pH was 7.0. The laboratory toxicity result indicated that all Flusilazole 40% EC, flucarbonitrile 50% WP, prochloraz 45% EW, carbendazim 80% WP, propiconazole 25% EC, and thiofuramide 24% SC showed higher effective on Penicillium spinulosum, with EC50 values of 0.007 8, 0.543 5, 0.058 8, 0.065 0, 0.631 7 and 4.732 3 mg·L-1, respectively.

Key words: chestnut, nut rot, Penicillium spinulosum, cultural conditions, fungicides

摘要： 板栗果仁腐烂病是板栗贮藏期主要病害，严重影响当地板栗产业的收益。小刺青霉菌（Penicillium spinulosum）是引起该病害的主要致病菌之一，为有效控制其对板栗储藏期间造成的危害，研究了其培养条件，并在此基础上对6种杀菌剂进行室内筛选。研究结果表明：小刺青霉菌在板栗培养基最适宜其生长，菌落直径为3.72 cm。PDA 培养基最适宜其产孢，产孢量为2.40×108 cell·L-1。氮源为酵母浸粉时最适合其菌丝生长和产孢，菌落生长和产孢量分别为3.42 cm和2.93×108 cell·L-1。可溶性淀粉为碳源时菌丝生长最快，菌落直径为3.35 cm，甘露醇为碳源时最利于其产孢，产孢量为1.97×108 cell·L-1。全黑暗条件最适宜菌丝生长，菌落直径为4.35 cm，而产孢对光照条件要求不严格，在任何光照条件下都能产孢。菌丝生长适宜温度是20 ℃；适宜pH为7.0。室内毒力测定结果表明：40%氟硅唑EC、50%咯菌腈WP、45%咪鲜胺EW、80%多菌灵WP、25%丙环唑EC、24%噻呋酰胺SC对小刺青霉均有较好的抑制作用，其EC50分别为0.007 8、0.543 5、0.058 8、0.065 0、0.631 7和4.732 3 mg·L-1。

关键词: 板栗, 果仁腐烂病, 小刺青霉菌, 培养条件, 杀菌剂