Abstract: Codonopsis pilosula polysaccharides can enhance immunity, and have better effects if they were made into the appropriate dosage. In order to make  stable W/O/W Codonopsis pilosula polysaccharide nanoemulsion immunoenhancer by two-step method, the pseudo-ternary phase diagram was used to determine the optimum volume ratio between emulsifier and co-emulsifier, emulsifier-co-emulsifier (Smix) and oil phase in the first step of preparing W/O colostrum. The type and dosage of emulsifier and co-emulsifier for preparing W/O/W Codonopsis pilosula polysaccharide nanoemulsion in the second step were determined by particle size and appearance. To identify the type of nanoemulsion, the pH value, particle size distribution and zeta potential were determined, then the microscopic morphology and stability were observed for studying properties of W/O/W Codonopsis pilosula polysaccharide nanoemulsion. The results showed that: the optimum volume ratio of W/O colostrum emulsifier to co-emulsifierand Smix to oil phase were 7∶3 and 5∶4, respectively. The optimum emulsifier and co-emulsifier of W/O/W Codonopsis pilosula polysaccharide nanoemulsion were HEL-40 and glycerol, and volume ratios were 1∶1 and 2∶1, respectively. The samples made according the two prescriptions (A′ and A″) were W/O/W with spherical appearance and uniform size without adhesion. The average particle sizes were (122.32±2.27) and (153.76±1.32) nm, Zeta potentials were (-1.12±0.21) and (-0.429±0.13) mV, and pH values were 6.3±0.1 and 5.5±0.1, respectively. The above results indicated that W/O/W Codonopsis pilosula polysaccharide nanoemulsion had good stability and simple operating method, which had potential application value.

Key words: Codonopsis pilosula polysaccharide, nanoemulsion, W/O/W, immunopotentiator

摘要： 党参多糖具有增强免疫作用，将其制备成适当的剂型将有助于更好地发挥作用。为了采用两步法制备出稳定的W/O/W型党参多糖纳米乳免疫增强剂，利用伪三元相图确定第一步制备W/O型初乳的乳化剂与助乳化剂最佳体积比、乳化剂-助乳化剂（Smix）与油相最佳体积比；通过考察粒径和外观，确定第二步制备W/O/W型党参多糖纳米乳最佳乳化剂、助乳化剂种类和用量。通过鉴别W/O/W型党参多糖纳米乳类型，测定pH、粒度分布、Zeta电位、观察纳米乳微观形态并考察样品稳定性。结果显示：W/O型初乳乳化剂与助乳化剂最佳体积比为7∶3，Smix与油相最佳体积比为5∶4； W/O/W型党参多糖纳米乳最佳乳化剂为HEL-40、助乳化剂为丙三醇，体积比为1∶1和2∶1；处方A′和A″条件下制备的样品为W/O/W型，外观呈球形，大小均匀无黏连，平均粒径分别为（122.32±2.27）和（153.76±1.32）nm，Zeta电位分别为（-1.12±0.21）和（-0.429±0.13）mV，pH分别为6.3±0.1和5.5±0.1，样品稳定性良好。结果表明，W/O/W型党参多糖纳米乳制备方法简单，稳定性好，具有潜在应用价值。

关键词: 党参多糖, 纳米乳, W/O/W, 免疫增强剂